CSTFT_HUMAN
ID CSTFT_HUMAN Reviewed; 616 AA.
AC Q9H0L4; B2RAR9; O75174; Q53HK6; Q7LGE8; Q8N6T1;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Cleavage stimulation factor subunit 2 tau variant;
DE AltName: Full=CF-1 64 kDa subunit tau variant;
DE AltName: Full=Cleavage stimulation factor 64 kDa subunit tau variant;
DE Short=CSTF 64 kDa subunit tau variant;
DE AltName: Full=TauCstF-64;
GN Name=CSTF2T; Synonyms=KIAA0689;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=12408968; DOI=10.1006/geno.2002.6862;
RA Dass B., McDaniel L., Schultz R.A., Attaya E., MacDonald C.C.;
RT "The gene CSTF2T, encoding the human variant CstF-64 polyadenylation
RT protein CstF-64, lacks introns and may be associated with male sterility.";
RL Genomics 80:509-514(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Coronary artery;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 70-616.
RC TISSUE=Brain;
RX PubMed=9734811; DOI=10.1093/dnares/5.3.169;
RA Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H.,
RA Nomura N., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. X. The
RT complete sequences of 100 new cDNA clones from brain which can code for
RT large proteins in vitro.";
RL DNA Res. 5:169-176(1998).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-320 AND SER-563, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: May play a significant role in AAUAAA-independent mRNA
CC polyadenylation in germ cells. Directly involved in the binding to pre-
CC mRNAs (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q9H0L4; Q9ULX6: AKAP8L; NbExp=3; IntAct=EBI-747012, EBI-357530;
CC Q9H0L4; Q9Y6H3: ATP23; NbExp=3; IntAct=EBI-747012, EBI-12811889;
CC Q9H0L4; Q9UMD9: COL17A1; NbExp=3; IntAct=EBI-747012, EBI-2528742;
CC Q9H0L4; P78358: CTAG1B; NbExp=3; IntAct=EBI-747012, EBI-1188472;
CC Q9H0L4; Q9GZU7: CTDSP1; NbExp=3; IntAct=EBI-747012, EBI-751587;
CC Q9H0L4; A1KXE4-2: FAM168B; NbExp=3; IntAct=EBI-747012, EBI-12193763;
CC Q9H0L4; Q12951-2: FOXI1; NbExp=3; IntAct=EBI-747012, EBI-12018822;
CC Q9H0L4; Q9P0K8: FOXJ2; NbExp=3; IntAct=EBI-747012, EBI-2869608;
CC Q9H0L4; O14964: HGS; NbExp=3; IntAct=EBI-747012, EBI-740220;
CC Q9H0L4; Q14847-2: LASP1; NbExp=3; IntAct=EBI-747012, EBI-9088686;
CC Q9H0L4; Q96KN3: PKNOX2; NbExp=5; IntAct=EBI-747012, EBI-2692890;
CC Q9H0L4; Q9H7B4: SMYD3; NbExp=3; IntAct=EBI-747012, EBI-347919;
CC Q9H0L4; Q99932-2: SPAG8; NbExp=3; IntAct=EBI-747012, EBI-11959123;
CC Q9H0L4; Q8IWL8: STH; NbExp=5; IntAct=EBI-747012, EBI-12843506;
CC Q9H0L4; Q08117-2: TLE5; NbExp=6; IntAct=EBI-747012, EBI-11741437;
CC Q9H0L4; Q96NM4-3: TOX2; NbExp=3; IntAct=EBI-747012, EBI-12815137;
CC Q9H0L4; Q9UMX0: UBQLN1; NbExp=7; IntAct=EBI-747012, EBI-741480;
CC Q9H0L4; Q9UMX0-2: UBQLN1; NbExp=3; IntAct=EBI-747012, EBI-10173939;
CC Q9H0L4; Q9UHD9: UBQLN2; NbExp=5; IntAct=EBI-747012, EBI-947187;
CC Q9H0L4; A0A1U9X8X8; NbExp=3; IntAct=EBI-747012, EBI-17234977;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
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DR EMBL; AY130299; AAN05429.1; -; mRNA.
DR EMBL; AL136747; CAB66681.1; -; mRNA.
DR EMBL; AK222574; BAD96294.1; -; mRNA.
DR EMBL; AK314318; BAG36966.1; -; mRNA.
DR EMBL; CH471083; EAW54142.1; -; Genomic_DNA.
DR EMBL; BC028239; AAH28239.1; -; mRNA.
DR EMBL; AB014589; BAA31664.1; -; mRNA.
DR CCDS; CCDS7245.1; -.
DR RefSeq; NP_056050.1; NM_015235.2.
DR AlphaFoldDB; Q9H0L4; -.
DR SMR; Q9H0L4; -.
DR BioGRID; 116881; 159.
DR IntAct; Q9H0L4; 43.
DR MINT; Q9H0L4; -.
DR STRING; 9606.ENSP00000332444; -.
DR GlyGen; Q9H0L4; 2 sites, 1 O-linked glycan (2 sites).
DR iPTMnet; Q9H0L4; -.
DR MetOSite; Q9H0L4; -.
DR PhosphoSitePlus; Q9H0L4; -.
DR BioMuta; CSTF2T; -.
DR DMDM; 71153234; -.
DR REPRODUCTION-2DPAGE; IPI00550906; -.
DR EPD; Q9H0L4; -.
DR jPOST; Q9H0L4; -.
DR MassIVE; Q9H0L4; -.
DR MaxQB; Q9H0L4; -.
DR PaxDb; Q9H0L4; -.
DR PeptideAtlas; Q9H0L4; -.
DR PRIDE; Q9H0L4; -.
DR ProteomicsDB; 80293; -.
DR Antibodypedia; 27941; 203 antibodies from 28 providers.
DR DNASU; 23283; -.
DR Ensembl; ENST00000331173.6; ENSP00000332444.4; ENSG00000177613.9.
DR GeneID; 23283; -.
DR KEGG; hsa:23283; -.
DR MANE-Select; ENST00000331173.6; ENSP00000332444.4; NM_015235.3; NP_056050.1.
DR UCSC; uc001jjp.4; human.
DR CTD; 23283; -.
DR DisGeNET; 23283; -.
DR GeneCards; CSTF2T; -.
DR HGNC; HGNC:17086; CSTF2T.
DR HPA; ENSG00000177613; Low tissue specificity.
DR MIM; 611968; gene.
DR neXtProt; NX_Q9H0L4; -.
DR OpenTargets; ENSG00000177613; -.
DR PharmGKB; PA134933809; -.
DR VEuPathDB; HostDB:ENSG00000177613; -.
DR eggNOG; KOG0108; Eukaryota.
DR GeneTree; ENSGT00940000161661; -.
DR HOGENOM; CLU_028601_3_1_1; -.
DR InParanoid; Q9H0L4; -.
DR OMA; GMQGTGI; -.
DR OrthoDB; 1455080at2759; -.
DR PhylomeDB; Q9H0L4; -.
DR TreeFam; TF314948; -.
DR PathwayCommons; Q9H0L4; -.
DR Reactome; R-HSA-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-HSA-72187; mRNA 3'-end processing.
DR Reactome; R-HSA-73856; RNA Polymerase II Transcription Termination.
DR Reactome; R-HSA-77595; Processing of Intronless Pre-mRNAs.
DR SignaLink; Q9H0L4; -.
DR BioGRID-ORCS; 23283; 11 hits in 1082 CRISPR screens.
DR GeneWiki; CSTF2T; -.
DR GenomeRNAi; 23283; -.
DR Pharos; Q9H0L4; Tbio.
DR PRO; PR:Q9H0L4; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q9H0L4; protein.
DR Bgee; ENSG00000177613; Expressed in endothelial cell and 210 other tissues.
DR Genevisible; Q9H0L4; HS.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0098789; P:pre-mRNA cleavage required for polyadenylation; IBA:GO_Central.
DR Gene3D; 1.10.20.70; -; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR025742; CSTF2_hinge.
DR InterPro; IPR026896; CSTF_C.
DR InterPro; IPR038192; CSTF_C_sf.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF14327; CSTF2_hinge; 1.
DR Pfam; PF14304; CSTF_C; 1.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW mRNA processing; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW RNA-binding.
FT CHAIN 1..616
FT /note="Cleavage stimulation factor subunit 2 tau variant"
FT /id="PRO_0000081534"
FT DOMAIN 16..94
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REPEAT 418..422
FT /note="1-1"
FT REPEAT 423..427
FT /note="1-2; approximate"
FT REPEAT 428..432
FT /note="1-3; approximate"
FT REPEAT 433..437
FT /note="1-4; approximate"
FT REPEAT 438..442
FT /note="1-5; approximate"
FT REPEAT 443..447
FT /note="1-6"
FT REPEAT 448..452
FT /note="1-7; approximate"
FT REPEAT 453..457
FT /note="1-8; approximate"
FT REPEAT 458..462
FT /note="1-9; approximate"
FT REPEAT 505..509
FT /note="2-1"
FT REPEAT 510..514
FT /note="2-2"
FT REPEAT 515..519
FT /note="2-3"
FT REPEAT 520..524
FT /note="2-4"
FT REPEAT 525..529
FT /note="2-5; approximate"
FT REPEAT 530..534
FT /note="2-6"
FT REPEAT 535..539
FT /note="2-7; approximate"
FT REPEAT 540..544
FT /note="2-8; approximate"
FT REPEAT 545..549
FT /note="2-9; approximate"
FT REGION 203..241
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 262..418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 418..462
FT /note="9 X 5 AA tandem repeats of M-E-T-R-[AG]"
FT REGION 505..549
FT /note="9 X 5 AA tandem repeats of G-[AT]-G-[MI]-Q"
FT REGION 542..573
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 550..573
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 320
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 563
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT CONFLICT 22
FT /note="N -> S (in Ref. 4; BAD96294)"
FT /evidence="ECO:0000305"
FT CONFLICT 553
FT /note="K -> R (in Ref. 6; AAH28239)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 616 AA; 64437 MW; 916BB9B0802AC847 CRC64;
MSSLAVRDPA MDRSLRSVFV GNIPYEATEE QLKDIFSEVG SVVSFRLVYD RETGKPKGYG
FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE LKSLGPAAPI IDSPYGDPID
PEDAPESITR AVASLPPEQM FELMKQMKLC VQNSHQEARN MLLQNPQLAY ALLQAQVVMR
IMDPEIALKI LHRKIHVTPL IPGKSQSVSV SGPGPGPGPG LCPGPNVLLN QQNPPAPQPQ
HLARRPVKDI PPLMQTPIQG GIPAPGPIPA AVPGAGPGSL TPGGAMQPQL GMPGVGPVPL
ERGQVQMSDP RAPIPRGPVT PGGLPPRGLL GDAPNDPRGG TLLSVTGEVE PRGYLGPPHQ
GPPMHHASGH DTRGPSSHEM RGGPLGDPRL LIGEPRGPMI DQRGLPMDGR GGRDSRAMET
RAMETEVLET RVMERRGMET CAMETRGMEA RGMDARGLEM RGPVPSSRGP MTGGIQGPGP
INIGAGGPPQ GPRQVPGISG VGNPGAGMQG TGIQGTGMQG AGIQGGGMQG AGIQGVSIQG
GGIQGGGIQG ASKQGGSQPS SFSPGQSQVT PQDQEKAALI MQVLQLTADQ IAMLPPEQRQ
SILILKEQIQ KSTGAS
