CSTL1_MOUSE
ID CSTL1_MOUSE Reviewed; 140 AA.
AC Q80Y72; Q3V2I7; Q64FK4; Q64FK5;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Cystatin-like 1 {ECO:0000312|MGI:MGI:2652834};
DE Flags: Precursor;
GN Name=Cstl1 {ECO:0000312|MGI:MGI:2652834};
GN Synonyms=Rcet1 {ECO:0000303|PubMed:18300157};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|Proteomes:UP000000589};
RN [1] {ECO:0000312|EMBL:AAU20811.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Testis {ECO:0000312|EMBL:AAU20811.1};
RX PubMed=18300157; DOI=10.1080/10425170601101501;
RA Xiang Y., Nie D.S., Zhang Q.J., Zhu W.B., Du J., Li W., Lu G.X.;
RT "Cloning, characterization and identification of Rcet1-v1 and Rcet1-v2, two
RT novel splice variants of mouse Rcet1 related to Cres subgroup of family 2
RT cystatins.";
RL DNA Seq. 19:13-19(2008).
RN [2] {ECO:0000312|EMBL:BAE21368.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAE21368.1};
RC TISSUE=Testis {ECO:0000312|EMBL:BAE21368.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3] {ECO:0000312|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4] {ECO:0000312|EMBL:EDL28541.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000312|EMBL:AAH48646.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis {ECO:0000312|EMBL:AAH48646.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=2 {ECO:0000303|PubMed:18300157};
CC IsoId=Q80Y72-1; Sequence=Displayed;
CC Name=1 {ECO:0000303|PubMed:18300157};
CC IsoId=Q80Y72-2; Sequence=VSP_058947;
CC -!- TISSUE SPECIFICITY: Highly expressed in testis where it localizes to
CC spermatogonium, spermatocyes and round spermatids. Not detected in
CC spermatozoa. Also detected in epididymis, cerebrum and pituitary.
CC {ECO:0000269|PubMed:18300157}.
CC -!- DEVELOPMENTAL STAGE: In testis, expression levels increase from
CC postnatal week 4 onwards with peak levels at postnatal week 8.
CC Expression remains high thereafter. {ECO:0000269|PubMed:18300157}.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000255,
CC ECO:0000255|RuleBase:RU362130, ECO:0000305}.
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DR EMBL; AY706755; AAU20811.1; -; mRNA.
DR EMBL; AY706756; AAU20812.1; -; mRNA.
DR EMBL; AY707915; AAU20814.1; -; mRNA.
DR EMBL; AK131797; BAE20810.1; -; mRNA.
DR EMBL; AK132807; BAE21368.1; -; mRNA.
DR EMBL; AL844519; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466519; EDL28541.1; -; Genomic_DNA.
DR EMBL; BC048646; AAH48646.1; -; mRNA.
DR CCDS; CCDS16844.1; -. [Q80Y72-1]
DR CCDS; CCDS89564.1; -. [Q80Y72-2]
DR RefSeq; NP_808323.1; NM_177655.3. [Q80Y72-1]
DR RefSeq; XP_006499365.1; XM_006499302.1. [Q80Y72-1]
DR RefSeq; XP_006499366.1; XM_006499303.3. [Q80Y72-1]
DR AlphaFoldDB; Q80Y72; -.
DR SMR; Q80Y72; -.
DR STRING; 10090.ENSMUSP00000105581; -.
DR GlyGen; Q80Y72; 1 site.
DR iPTMnet; Q80Y72; -.
DR PhosphoSitePlus; Q80Y72; -.
DR PaxDb; Q80Y72; -.
DR PRIDE; Q80Y72; -.
DR ProteomicsDB; 277905; -. [Q80Y72-1]
DR Antibodypedia; 42866; 96 antibodies from 20 providers.
DR DNASU; 228756; -.
DR Ensembl; ENSMUST00000109952; ENSMUSP00000105578; ENSMUSG00000055177. [Q80Y72-1]
DR Ensembl; ENSMUST00000109955; ENSMUSP00000105581; ENSMUSG00000055177. [Q80Y72-1]
DR GeneID; 228756; -.
DR KEGG; mmu:228756; -.
DR UCSC; uc008mtj.1; mouse. [Q80Y72-1]
DR UCSC; uc008mtk.1; mouse.
DR CTD; 128817; -.
DR MGI; MGI:2652834; Cstl1.
DR VEuPathDB; HostDB:ENSMUSG00000055177; -.
DR eggNOG; ENOG502SAEJ; Eukaryota.
DR GeneTree; ENSGT00900000141212; -.
DR HOGENOM; CLU_118168_2_2_1; -.
DR InParanoid; Q80Y72; -.
DR OMA; RWGGFQE; -.
DR OrthoDB; 1565344at2759; -.
DR PhylomeDB; Q80Y72; -.
DR BioGRID-ORCS; 228756; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Cstl1; mouse.
DR PRO; PR:Q80Y72; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q80Y72; protein.
DR Bgee; ENSMUSG00000055177; Expressed in spermatid and 21 other tissues.
DR ExpressionAtlas; Q80Y72; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR042921; CSTL1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR PANTHER; PTHR47887; PTHR47887; 1.
DR Pfam; PF00031; Cystatin; 1.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Protease inhibitor;
KW Reference proteome; Secreted; Signal; Thiol protease inhibitor.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..140
FT /note="Cystatin-like 1"
FT /id="PRO_5009369047"
FT DOMAIN 32..126
FT /note="Cystatin"
FT /evidence="ECO:0000255"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 94..104
FT /evidence="ECO:0000250|UniProtKB:P19313"
FT DISULFID 117..137
FT /evidence="ECO:0000250|UniProtKB:P19313"
FT VAR_SEQ 26..77
FT /note="Missing (in isoform 1)"
FT /id="VSP_058947"
FT CONFLICT 25
FT /note="V -> L (in Ref. 1; AAU20812)"
FT /evidence="ECO:0000305"
FT CONFLICT 77
FT /note="L -> M (in Ref. 2; BAE20810)"
FT /evidence="ECO:0000305"
FT CONFLICT 85
FT /note="V -> A (in Ref. 2; BAE20810)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 140 AA; 16199 MW; 32633E99C4697DA0 CRC64;
MEMKARGLRI PLLLLLVTVV VMAKVNHIQR WGGFKEKAMS KKNINSTLHF FIRSYNNASN
DTYLYQVQKL IQGQMQLTTG VEYLVTVKIG RTKCKKNETK KASCPLQSSK LKKSLICKSL
IYSVPWMNYY QLWNNSCQES
