CSTP1_MOUSE
ID CSTP1_MOUSE Reviewed; 331 AA.
AC Q8BHR8; Q3TPK0; Q5HZI4;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Centriolar satellite-associated tubulin polyglutamylase complex regulator 1;
GN Name=Cstpp1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Corpora quadrigemina, and Spinal ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=FVB/N-3; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-319, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic brain;
RX PubMed=15345747; DOI=10.1074/mcp.m400085-mcp200;
RA Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
RT "Phosphoproteomic analysis of the developing mouse brain.";
RL Mol. Cell. Proteomics 3:1093-1101(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-319, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Regulator of the tubulin polyglutamylase complex (TPGC) that
CC controls cytoskeletal organization, nuclear shape, and cilium
CC disassembly by balancing microtubule and actin assembly. Regulates the
CC assembly and stability of the TPGC and thereby modulates
CC polyglutamylation of the microtubule, which antagonizes MAP4 binding.
CC {ECO:0000250|UniProtKB:Q9H6J7}.
CC -!- SUBUNIT: Interacts with PCM1. Interacts with TTLL1, TPGS1, TPGS2 and
CC LRRC49; the interactions link CSTPP1 to the complex TPGC. Binds to
CC alpha-tubulin. {ECO:0000250|UniProtKB:Q9H6J7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome, centriolar satellite
CC {ECO:0000250|UniProtKB:Q9H6J7}. Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q9H6J7}. Note=Associated with microtubules.
CC {ECO:0000250|UniProtKB:Q9H6J7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BHR8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BHR8-2; Sequence=VSP_024002, VSP_024003;
CC -!- SIMILARITY: Belongs to the CSTPP1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE37736.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK045977; BAC32558.1; -; mRNA.
DR EMBL; AK164320; BAE37736.1; ALT_FRAME; mRNA.
DR EMBL; AL732478; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC085239; AAH85239.1; -; mRNA.
DR EMBL; BC089006; AAH89006.1; -; mRNA.
DR CCDS; CCDS16431.1; -. [Q8BHR8-1]
DR CCDS; CCDS79823.1; -. [Q8BHR8-2]
DR RefSeq; NP_001298073.1; NM_001311144.1. [Q8BHR8-2]
DR RefSeq; NP_780332.1; NM_175123.4. [Q8BHR8-1]
DR AlphaFoldDB; Q8BHR8; -.
DR STRING; 10090.ENSMUSP00000099654; -.
DR iPTMnet; Q8BHR8; -.
DR PhosphoSitePlus; Q8BHR8; -.
DR MaxQB; Q8BHR8; -.
DR PaxDb; Q8BHR8; -.
DR PRIDE; Q8BHR8; -.
DR Antibodypedia; 42909; 42 antibodies from 17 providers.
DR DNASU; 228356; -.
DR Ensembl; ENSMUST00000064652; ENSMUSP00000067770; ENSMUSG00000040591. [Q8BHR8-2]
DR Ensembl; ENSMUST00000102594; ENSMUSP00000099654; ENSMUSG00000040591. [Q8BHR8-1]
DR GeneID; 228356; -.
DR KEGG; mmu:228356; -.
DR UCSC; uc008kvs.1; mouse. [Q8BHR8-2]
DR UCSC; uc008kvt.1; mouse. [Q8BHR8-1]
DR CTD; 79096; -.
DR MGI; MGI:1915079; 1110051M20Rik.
DR VEuPathDB; HostDB:ENSMUSG00000040591; -.
DR eggNOG; ENOG502QRVN; Eukaryota.
DR GeneTree; ENSGT00390000012935; -.
DR HOGENOM; CLU_064579_0_0_1; -.
DR InParanoid; Q8BHR8; -.
DR OMA; NIHHRRK; -.
DR OrthoDB; 1103030at2759; -.
DR PhylomeDB; Q8BHR8; -.
DR TreeFam; TF329168; -.
DR BioGRID-ORCS; 228356; 0 hits in 72 CRISPR screens.
DR PRO; PR:Q8BHR8; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8BHR8; protein.
DR Bgee; ENSMUSG00000040591; Expressed in retinal neural layer and 258 other tissues.
DR ExpressionAtlas; Q8BHR8; baseline and differential.
DR Genevisible; Q8BHR8; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR InterPro; IPR038968; C11orf49.
DR PANTHER; PTHR34252; PTHR34252; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Microtubule; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..331
FT /note="Centriolar satellite-associated tubulin
FT polyglutamylase complex regulator 1"
FT /id="PRO_0000281427"
FT REGION 1..225
FT /note="Required for interaction with TPGS1, LRRC49, and
FT TTLL1"
FT /evidence="ECO:0000250|UniProtKB:Q9H6J7"
FT REGION 1..111
FT /note="Required for interaction with PCM1"
FT /evidence="ECO:0000250|UniProtKB:Q9H6J7"
FT REGION 112..331
FT /note="Required for interaction with TPGS2"
FT /evidence="ECO:0000250|UniProtKB:Q9H6J7"
FT REGION 292..331
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 319
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:15345747,
FT ECO:0007744|PubMed:21183079"
FT VAR_SEQ 275..319
FT /note="LVQVPGLVNSITATSEASCLPSRTPPRVGSPWKPLHRSRKLDAES -> VVR
FT SRLYRTPQQQRGKEPGAFGFEGRDKLEAPETVPSALAPTPNPYRWEPRE (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_024002"
FT VAR_SEQ 320..331
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_024003"
SQ SEQUENCE 331 AA; 37550 MW; FCAD8FE4AADD1449 CRC64;
MLSPERLALP DYEYLAQRHV LTYMEDAVCQ LLENREDISQ YGIARFFTDY FNSVCQGTHI
LFREFSFIQA TPHNRASFLR AFWRCFRTVG KNGDLLTMRE YHCLLQLLCP DFPLELTQKA
ARIVLMDDAM DCLMSFSDFL FAFQIQFYYS EFLESVAAIY QDLLSGKNPN TVIVPTSSSG
QHRQRPALGD AGMLDGVEAS LFYQRLENLC DRHKYSCPPP ALVKEILSNV QRLTFYGFLV
ALSKHHGINQ ALGALPDKGD LMHDPAMDEE LERLLVQVPG LVNSITATSE ASCLPSRTPP
RVGSPWKPLH RSRKLDAESD GSTEETDESE T
