CSTR2_ARATH
ID CSTR2_ARATH Reviewed; 406 AA.
AC Q8GY97; C0Z289; F4IQ88; Q0WNS7; Q9ZW71;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=CMP-sialic acid transporter 2;
DE Short=CMP-SA-Tr 2;
DE Short=CMP-Sia-Tr 2;
GN OrderedLocusNames=At2g43240; ORFNames=F14B2.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=15456736; DOI=10.1093/glycob/cwh159;
RA Bakker H., Routier F., Oelmann S., Jordi W., Lommen A., Gerardy-Schahn R.,
RA Bosch D.;
RT "Molecular cloning of two Arabidopsis UDP-galactose transporters by
RT complementation of a deficient Chinese hamster ovary cell line.";
RL Glycobiology 15:193-201(2005).
RN [7]
RP GENE FAMILY.
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
CC -!- FUNCTION: Sugar transporter involved in the transport of CMP-sialic
CC acid from the cytoplasm into the Golgi. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8GY97-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8GY97-2; Sequence=VSP_042457, VSP_042458;
CC Name=3;
CC IsoId=Q8GY97-3; Sequence=VSP_042455;
CC Name=4;
CC IsoId=Q8GY97-4; Sequence=VSP_042456;
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. CMP-
CC Sialate:CMP antiporter (TC 2.A.7.12) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC64313.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004450; AAC64313.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10235.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10236.1; -; Genomic_DNA.
DR EMBL; AK117778; BAC42425.1; -; mRNA.
DR EMBL; AK318703; BAH56818.1; -; mRNA.
DR EMBL; AK176729; BAD44492.1; -; mRNA.
DR EMBL; AK229359; BAF01222.1; -; mRNA.
DR PIR; G84863; G84863.
DR RefSeq; NP_001118506.1; NM_001125034.1. [Q8GY97-4]
DR RefSeq; NP_181853.3; NM_129886.4. [Q8GY97-1]
DR AlphaFoldDB; Q8GY97; -.
DR SMR; Q8GY97; -.
DR STRING; 3702.AT2G43240.1; -.
DR PaxDb; Q8GY97; -.
DR PRIDE; Q8GY97; -.
DR ProteomicsDB; 222718; -. [Q8GY97-1]
DR EnsemblPlants; AT2G43240.1; AT2G43240.1; AT2G43240. [Q8GY97-1]
DR EnsemblPlants; AT2G43240.2; AT2G43240.2; AT2G43240. [Q8GY97-4]
DR GeneID; 818925; -.
DR Gramene; AT2G43240.1; AT2G43240.1; AT2G43240. [Q8GY97-1]
DR Gramene; AT2G43240.2; AT2G43240.2; AT2G43240. [Q8GY97-4]
DR KEGG; ath:AT2G43240; -.
DR Araport; AT2G43240; -.
DR TAIR; locus:2040976; AT2G43240.
DR eggNOG; KOG2234; Eukaryota.
DR HOGENOM; CLU_035460_0_0_1; -.
DR InParanoid; Q8GY97; -.
DR OMA; SSCVVMI; -.
DR OrthoDB; 754910at2759; -.
DR PhylomeDB; Q8GY97; -.
DR PRO; PR:Q8GY97; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8GY97; baseline and differential.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0015165; F:pyrimidine nucleotide-sugar transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015136; F:sialic acid transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0015739; P:sialic acid transport; ISS:UniProtKB.
DR InterPro; IPR007271; Nuc_sug_transpt.
DR PANTHER; PTHR10231; PTHR10231; 1.
DR Pfam; PF04142; Nuc_sug_transp; 1.
DR PIRSF; PIRSF005799; UDP-gal_transpt; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Golgi apparatus; Membrane; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..406
FT /note="CMP-sialic acid transporter 2"
FT /id="PRO_0000416025"
FT TOPO_DOM 1..41
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..75
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 97..148
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..172
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 194..201
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..245
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..282
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304..323
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..344
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 345..406
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..121
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_042455"
FT VAR_SEQ 97..115
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_042456"
FT VAR_SEQ 349..359
FT /note="FFSPLSKAKDE -> VMLYKPFLKPL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_042457"
FT VAR_SEQ 360..406
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_042458"
SQ SEQUENCE 406 AA; 44963 MW; 8D704D0BE8097C93 CRC64;
MKNGMAECSV CRSRLVSPSS KAISRAYDNY NYKIRVSSKQ RALNVFLVVG DCMLVGLQPV
LVYMSKVDGK FNFSPISVNF LTEIAKVIFA MVMLLFQARH QKVGEKPLLS LSTFVQAARN
NMLLAVPAGL YAINNYLKFT MQLYFNPATV KMLSNLKVLV IAVLLKMIMK RRFSIIQWEA
LALLLIGISI NQLRSLPEGA TTVAVPIATG AYICTFIFVT VPSLASVYNE YALKSQYDTS
IYLQNLFLYG YGAIFNFLGI LGTVIYKGPG SFDILQGHSR ATMFLILNNA AQGILSSFFF
KYADTILKKY SSTVATIFTG IASAALFGHI LTMNFLLGIS IVFISMHQFF SPLSKAKDEQ
QNGNIELVDA KDGHRAKDSF INMAAGATEE ASHRIESDDR VPLLPR
