ID   CSY2_PECAS              Reviewed;         310 AA.
AC   Q6D0W7;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=CRISPR-associated protein Csy2;
GN   Name=csy2; OrderedLocusNames=ECA3682;
OS   Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS   carotovora subsp. atroseptica).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=218491;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA   Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA   Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA   Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA   Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA   Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA   Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT   "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT   subsp. atroseptica and characterization of virulence factors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
RN   [2]
RP   INDUCTION, AND OPERON STRUCTURE.
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=21519197; DOI=10.4161/rna.8.3.15190;
RA   Przybilski R., Richter C., Gristwood T., Clulow J.S., Vercoe R.B.,
RA   Fineran P.C.;
RT   "Csy4 is responsible for CRISPR RNA processing in Pectobacterium
RT   atrosepticum.";
RL   RNA Biol. 8:517-528(2011).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CSY1, AND SUBUNIT.
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=23226499; DOI=10.1371/journal.pone.0049549;
RA   Richter C., Gristwood T., Clulow J.S., Fineran P.C.;
RT   "In vivo protein interactions and complex formation in the Pectobacterium
RT   atrosepticum subtype I-F CRISPR/Cas System.";
RL   PLoS ONE 7:E49549-E49549(2012).
RN   [4]
RP   FUNCTION, AND BIOTECHNOLOGY.
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=23637624; DOI=10.1371/journal.pgen.1003454;
RA   Vercoe R.B., Chang J.T., Dy R.L., Taylor C., Gristwood T., Clulow J.S.,
RA   Richter C., Przybilski R., Pitman A.R., Fineran P.C.;
RT   "Cytotoxic chromosomal targeting by CRISPR/Cas systems can reshape
RT   bacterial genomes and expel or remodel pathogenicity islands.";
RL   PLoS Genet. 9:E1003454-E1003454(2013).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       {ECO:0000269|PubMed:23637624}.
CC   -!- SUBUNIT: Interacts directly with Cys1; part of the probable Csy
CC       ribonucleoprotein complex with cys1, cys2, csy3, cas6f and crRNA.
CC       {ECO:0000269|PubMed:23226499}.
CC   -!- INDUCTION: Expressed in late exponential phase (other phases not
CC       tested); part of a large cas-CRISPR3 polycistronic operon.
CC       {ECO:0000269|PubMed:21519197}.
CC   -!- BIOTECHNOLOGY: If the spacer DNA has a perfect match in the chromosome
CC       then toxicity results. Suppression of the toxic effects occurs via
CC       mutations in the CRISPR/Cas machinery, or via target deletion, which
CC       might contribute to genome plasticity. This CRISPR/Cas system can be
CC       used to remove genomic islands, and possibly other genomic regions.
CC       {ECO:0000269|PubMed:23637624}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated Csy2 family.
CC       {ECO:0000305}.
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DR   EMBL; BX950851; CAG76580.1; -; Genomic_DNA.
DR   RefSeq; WP_011095182.1; NC_004547.2.
DR   AlphaFoldDB; Q6D0W7; -.
DR   SMR; Q6D0W7; -.
DR   STRING; 218491.ECA3682; -.
DR   EnsemblBacteria; CAG76580; CAG76580; ECA3682.
DR   GeneID; 57210352; -.
DR   KEGG; eca:ECA3682; -.
DR   PATRIC; fig|218491.5.peg.3734; -.
DR   eggNOG; ENOG502Z9HE; Bacteria.
DR   HOGENOM; CLU_073578_0_0_6; -.
DR   OMA; MWALERK; -.
DR   OrthoDB; 798820at2; -.
DR   Proteomes; UP000007966; Chromosome.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR013398; CRISPR-assoc_prot_Csy2.
DR   Pfam; PF09614; Cas_Csy2; 1.
DR   TIGRFAMs; TIGR02565; cas_Csy2; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Reference proteome.
FT   CHAIN           1..310
FT                   /note="CRISPR-associated protein Csy2"
FT                   /id="PRO_0000430244"
SQ   SEQUENCE   310 AA;  34860 MW;  513E62263022ACBC CRC64;
     MSTLIILRRI QVENANAIAG LTYGFPAITH FLGFTHALSR KLQASHGLTL EGCGVVSHQH
     QLHAYGSSWE RSFALTRNPL TKEAKTAAFN EEGRMHMTVS LLIRCDGQIP ADTTALCEHL
     KQQAQCQRLA GGTVIDIERV TVQSLPVDEA ETRGVMRRLL PGFVLRDRTS LLHRHFQTLQ
     QAKPQAEMID AWLDFAALKM QAERDPSDET VQWKYLPKPG DGGFLTPLMI GYRAISPLYA
     PGEVDKTRDP HTPFCFAEAA YGIGEWQGAH RISDISQILW EYDYQNGDYH CRQVADTHSV
     AEDTSYEFDY