ID   CSY3_PECAS              Reviewed;         337 AA.
AC   Q6D0W6;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=CRISPR-associated protein Csy3;
GN   Name=csy3; OrderedLocusNames=ECA3683;
OS   Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS   carotovora subsp. atroseptica).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=218491;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA   Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA   Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA   Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA   Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA   Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA   Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT   "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT   subsp. atroseptica and characterization of virulence factors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
RN   [2]
RP   INDUCTION, AND OPERON STRUCTURE.
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=21519197; DOI=10.4161/rna.8.3.15190;
RA   Przybilski R., Richter C., Gristwood T., Clulow J.S., Vercoe R.B.,
RA   Fineran P.C.;
RT   "Csy4 is responsible for CRISPR RNA processing in Pectobacterium
RT   atrosepticum.";
RL   RNA Biol. 8:517-528(2011).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBUNIT.
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=23226499; DOI=10.1371/journal.pone.0049549;
RA   Richter C., Gristwood T., Clulow J.S., Fineran P.C.;
RT   "In vivo protein interactions and complex formation in the Pectobacterium
RT   atrosepticum subtype I-F CRISPR/Cas System.";
RL   PLoS ONE 7:E49549-E49549(2012).
RN   [4]
RP   FUNCTION, AND BIOTECHNOLOGY.
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=23637624; DOI=10.1371/journal.pgen.1003454;
RA   Vercoe R.B., Chang J.T., Dy R.L., Taylor C., Gristwood T., Clulow J.S.,
RA   Richter C., Przybilski R., Pitman A.R., Fineran P.C.;
RT   "Cytotoxic chromosomal targeting by CRISPR/Cas systems can reshape
RT   bacterial genomes and expel or remodel pathogenicity islands.";
RL   PLoS Genet. 9:E1003454-E1003454(2013).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       {ECO:0000269|PubMed:23637624}.
CC   -!- SUBUNIT: Probable multimer. Part of the probable Csy ribonucleoprotein
CC       complex with cys1, cys2, csy3, cas6f and crRNA.
CC       {ECO:0000269|PubMed:23226499}.
CC   -!- INDUCTION: Expressed in late exponential phase (other phases not
CC       tested); part of a large cas-CRISPR3 polycistronic operon.
CC       {ECO:0000269|PubMed:21519197}.
CC   -!- BIOTECHNOLOGY: If the spacer DNA has a perfect match in the chromosome
CC       then toxicity results. Suppression of the toxic effects occurs via
CC       mutations in the CRISPR/Cas machinery, or via target deletion, which
CC       might contribute to genome plasticity. This CRISPR/Cas system can be
CC       used to remove genomic islands, and possibly other genomic regions.
CC       {ECO:0000269|PubMed:23637624}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated Csy3 family.
CC       {ECO:0000305}.
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DR   EMBL; BX950851; CAG76581.1; -; Genomic_DNA.
DR   RefSeq; WP_011095183.1; NC_004547.2.
DR   AlphaFoldDB; Q6D0W6; -.
DR   SMR; Q6D0W6; -.
DR   STRING; 218491.ECA3683; -.
DR   EnsemblBacteria; CAG76581; CAG76581; ECA3683.
DR   GeneID; 57210353; -.
DR   KEGG; eca:ECA3683; -.
DR   PATRIC; fig|218491.5.peg.3735; -.
DR   eggNOG; ENOG502Z7PG; Bacteria.
DR   HOGENOM; CLU_063672_0_0_6; -.
DR   OMA; CNDQDYQ; -.
DR   OrthoDB; 1210545at2; -.
DR   Proteomes; UP000007966; Chromosome.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR013399; CRISPR-assoc_prot_Csy3.
DR   Pfam; PF09615; Cas_Csy3; 1.
DR   TIGRFAMs; TIGR02566; cas_Csy3; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Reference proteome.
FT   CHAIN           1..337
FT                   /note="CRISPR-associated protein Csy3"
FT                   /id="PRO_0000430245"
SQ   SEQUENCE   337 AA;  36910 MW;  0BB6C992840FE58B CRC64;
     MAKAATTLKT ASVLAFERKL ANSDALMYAG NWAQQDNWTA IAIQEKSVRG TISNRLKNAL
     TSDPAKLDAE IQKANLQKVD VAALPFGADT LKIVFTLRVL GNLAQPSVCN DQDYQTALGD
     IITGYAQEQG FSTLAARYAE NIANGRFLWR NRVGAEAIRV VVTKKGERSW EFNGEDYSLR
     QFSQPAGDLA ALTQAIEKGL AGDASALFTV EAYVQLGNGQ EVFPSQELVL DEKARNGKSK
     ILYQVNDVAA IHSQKIGNAL RTIDDWYPAA DEAGPIAVEP YGSVTSRGKA YRQPREKMDF
     YTLLDNWVIK GDVPMPEQQH YVIATLIRGG VFGEKGE