ID   CT111_LACTA             Reviewed;         129 AA.
AC   P85247; B3W6I6;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 2.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=M-zodatoxin-Lt8h;
DE            Short=M-ZDTX-Lt8h;
DE   AltName: Full=Cytoinsectotoxin-1h;
DE            Short=CIT-1h;
DE   Flags: Precursor;
GN   Name=cit 1-11;
OS   Lachesana tarabaevi (Spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Entelegynae incertae sedis; Zodariidae;
OC   Lachesana.
OX   NCBI_TaxID=379576;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 61-129, FUNCTION,
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Venom {ECO:0000269|PubMed:18215128}, and Venom gland;
RX   PubMed=18215128; DOI=10.1042/bj20071123;
RA   Vassilevski A.A., Kozlov S.A., Samsonova O.V., Egorova N.S., Karpunin D.V.,
RA   Pluzhnikov K.A., Feofanov A.V., Grishin E.V.;
RT   "Cyto-insectotoxins, a novel class of cytolytic and insecticidal peptides
RT   from spider venom.";
RL   Biochem. J. 411:687-696(2008).
CC   -!- FUNCTION: Insecticidal, cytolytic and antimicrobial peptide. Has
CC       insecticidal activity against the flesh fly S.carnaria. Has
CC       antibacterial activity against the Gram-negative bacteria E.coli. Forms
CC       voltage-dependent, ion-permeable channels in membranes. At high
CC       concentration causes cell membrane lysis.
CC       {ECO:0000269|PubMed:18215128}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18215128}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:18215128}.
CC   -!- SIMILARITY: Belongs to the cationic peptide 06 (cytoinsectotoxin)
CC       family. {ECO:0000305}.
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DR   EMBL; FM165481; CAQ63557.1; -; mRNA.
DR   AlphaFoldDB; P85247; -.
DR   ArachnoServer; AS000565; M-zodatoxin-Lt8h.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0051838; P:cytolysis by host of symbiont cells; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; ISS:UniProtKB.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing; Hemolysis;
KW   Secreted; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..60
FT                   /evidence="ECO:0000269|PubMed:18215128"
FT                   /id="PRO_0000366081"
FT   CHAIN           61..129
FT                   /note="M-zodatoxin-Lt8h"
FT                   /id="PRO_0000337165"
SQ   SEQUENCE   129 AA;  14550 MW;  5541E4A5A34244DF CRC64;
     MKYFVVALAL VAAFACIAES KPAESEHELA EVEEENELAD LEDAVWLEDL ADLSDLEETR
     GFFGNAWKKI KGKAEKFFRK KAAKIIAKKE GITKEEAEAK VDTMSKKQIK VYLLKHYGKK
     ALQKASEKL