ID   CT53_CONMR              Reviewed;          64 AA.
AC   Q6PN83;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Conotoxin mr5.3 {ECO:0000303|PubMed:15733570};
DE   AltName: Full=Gla-MrIV {ECO:0000303|PubMed:15194478};
DE   Flags: Precursor;
OS   Conus marmoreus (Marble cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Conus.
OX   NCBI_TaxID=42752;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom duct;
RX   PubMed=15733570; DOI=10.1016/j.toxicon.2004.12.008;
RA   Han Y.-H., Wang Q., Jiang H., Miao X.-W., Chen J.-S., Chi C.-W.;
RT   "Sequence diversity of T-superfamily conotoxins from Conus marmoreus.";
RL   Toxicon 45:481-487(2005).
RN   [2]
RP   PROTEIN SEQUENCE, GAMMA-CARBOXYGLUTAMATION AT GLU-56 AND GLU-60, MASS
RP   SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=15194478; DOI=10.1016/j.bbrc.2004.05.088;
RA   Hansson K., Furie B., Furie B.C., Stenflo J.;
RT   "Isolation and characterization of three novel Gla-containing Conus
RT   marmoreus venom peptides, one with a novel cysteine pattern.";
RL   Biochem. Biophys. Res. Commun. 319:1081-1087(2004).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15194478}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:15194478}.
CC   -!- DOMAIN: The cysteine framework is V (CC-CC). {ECO:0000305}.
CC   -!- PTM: Contains 2 disulfide bonds that can be either 'C1-C3, C2-C4' or
CC       'C1-C4, C2-C3', since these disulfide connectivities have been observed
CC       for conotoxins with cysteine framework V (for examples, see AC P0DQQ7
CC       and AC P81755). {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=1595.51; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:15194478};
CC   -!- SIMILARITY: Belongs to the conotoxin T superfamily. {ECO:0000305}.
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DR   EMBL; AY591767; AAT01631.1; -; mRNA.
DR   AlphaFoldDB; Q6PN83; -.
DR   ConoServer; 858; Gla-MrIV precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR031565; T-conotoxin.
DR   Pfam; PF16981; Chi-conotoxin; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Gamma-carboxyglutamic acid; Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..48
FT                   /evidence="ECO:0000305|PubMed:15194478"
FT                   /id="PRO_0000035035"
FT   PEPTIDE         51..63
FT                   /note="Conotoxin mr5.3"
FT                   /evidence="ECO:0000269|PubMed:15194478"
FT                   /id="PRO_0000035036"
FT   MOD_RES         56
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15194478"
FT   MOD_RES         60
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000269|PubMed:15194478"
SQ   SEQUENCE   64 AA;  7224 MW;  34CA3203D24A76DB CRC64;
     MRCVPVFVIL LLLIASVPSV DAQLKTKDDM PLASSHANVK RTLQILRNKR CCITFESCCE
     FDLK