CT5AS_CONTE
ID CT5AS_CONTE Reviewed; 67 AA.
AC P81755; Q9U6Z7;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2002, sequence version 2.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Epsilon-conotoxin TxVA {ECO:0000305};
DE AltName: Full=Epsilon-TxIX {ECO:0000303|PubMed:10318957, ECO:0000303|PubMed:10679974};
DE AltName: Full=Tx-012 {ECO:0000312|EMBL:AAG60386.1};
DE AltName: Full=Tx5.2 {ECO:0000303|PubMed:10521453};
DE AltName: Full=tx5a {ECO:0000303|PubMed:10521453};
DE Flags: Precursor;
OS Conus textile (Cloth-of-gold cone).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Cylinder.
OX NCBI_TaxID=6494;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-63, MASS SPECTROMETRY,
RP SUBCELLULAR LOCATION, GAMMA-CARBOXYGLUTAMATION AT GLU-51 AND GLU-54,
RP BROMINATION AT TRP-57, HYDROXYLATION AT PRO-63, AND GLYCOSYLATION AT
RP THR-60.
RC TISSUE=Venom, and Venom duct;
RX PubMed=10521453; DOI=10.1074/jbc.274.43.30664;
RA Walker C.S., Steel D., Jacobsen R.B., Lirazan M.B., Cruz L.J., Hooper D.,
RA Shetty R., DelaCruz R.C., Nielsen J.S., Zhou L.M., Bandyopadhyay P.,
RA Craig A.G., Olivera B.M.;
RT "The T-superfamily of conotoxins.";
RL J. Biol. Chem. 274:30664-30671(1999).
RN [2]
RP ERRATUM OF PUBMED:10521453.
RA Walker C.S., Steel D., Jacobsen R.B., Lirazan M.B., Cruz L.J., Hooper D.,
RA Shetty R., DelaCruz R.C., Nielsen J.S., Zhou L.M., Bandyopadhyay P.,
RA Craig A.G., Olivera B.M.;
RL J. Biol. Chem. 274:36030-36030(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom duct;
RX PubMed=11158371; DOI=10.1093/oxfordjournals.molbev.a003786;
RA Conticello S.G., Gilad Y., Avidan N., Ben-Asher E., Levy Z., Fainzilber M.;
RT "Mechanisms for evolving hypervariability: the case of conopeptides.";
RL Mol. Biol. Evol. 18:120-131(2001).
RN [4]
RP PROTEIN SEQUENCE OF 51-63, FUNCTION, GLYCOSYLATION AT THR-60,
RP GAMMA-CARBOXYGLUTAMATION AT GLU-51 AND GLU-54, BROMINATION AT TRP-57,
RP HYDROXYLATION AT PRO-63, STRUCTURE BY NMR OF 51-63, AND DISULFIDE BONDS.
RC TISSUE=Venom;
RX PubMed=10318957; DOI=10.1073/pnas.96.10.5758;
RA Rigby A.C., Lucas-Meunier E., Kalume D.E., Czerwiec E., Hambe B.,
RA Dahlqvist I., Fossier P., Baux G., Roepstorff P., Baleja J.D., Furie B.C.,
RA Furie B., Stenflo J.P.;
RT "A conotoxin from Conus textile with unusual posttranslational
RT modifications reduces presynaptic Ca2+ influx.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:5758-5763(1999).
RN [5]
RP PROTEIN SEQUENCE OF 51-63, AND MASS SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=10679974;
RX DOI=10.1002/(sici)1096-9888(200002)35:2<145::aid-jms922>3.0.co;2-i;
RA Kalume D.E., Stenflo J.P., Czerwiec E., Hambe B., Furie B.C., Furie B.,
RA Roepstorff P.;
RT "Structure determination of two conotoxins from Conus textile by a
RT combination of matrix-assisted laser desorption/ionization time-of-flight
RT and electrospray ionization mass spectrometry and biochemical methods.";
RL J. Mass Spectrom. 35:145-156(2000).
CC -!- FUNCTION: Epsilon-conotoxins act at presynaptic membranes, blocking the
CC calcium channels or G protein-coupled receptors. Causes hyperactivity
CC upon intracranial injection into mice. Causes dorsal fins drooping in
CC fish. {ECO:0000269|PubMed:10318957}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10521453}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC {ECO:0000305|PubMed:10521453}.
CC -!- DOMAIN: The cysteine framework is V (CC-CC). {ECO:0000305}.
CC -!- PTM: O-glycan consists of the disaccharide Gal-GalNAc.
CC {ECO:0000269|PubMed:10318957}.
CC -!- MASS SPECTROMETRY: Mass=1929.4; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10521453, ECO:0000269|PubMed:10679974};
CC -!- MASS SPECTROMETRY: Mass=1929.43; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10521453, ECO:0000269|PubMed:10679974};
CC -!- SIMILARITY: Belongs to the conotoxin T superfamily. {ECO:0000305}.
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DR EMBL; AF167167; AAF03687.1; -; mRNA.
DR EMBL; AF214958; AAG60386.1; -; mRNA.
DR PIR; E59147; E59147.
DR PDB; 1WCT; NMR; -; A=51-63.
DR PDBsum; 1WCT; -.
DR AlphaFoldDB; P81755; -.
DR SMR; P81755; -.
DR TCDB; 8.B.4.1.8; the conotoxin t (conotoxin t) family.
DR iPTMnet; P81755; -.
DR ConoServer; 645; TxVA precursor.
DR EvolutionaryTrace; P81755; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR031565; T-conotoxin.
DR Pfam; PF16981; Chi-conotoxin; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bromination; Calcium channel impairing toxin;
KW Direct protein sequencing; Disulfide bond; Gamma-carboxyglutamic acid;
KW Glycoprotein; Hydroxylation; Ion channel impairing toxin; Neurotoxin;
KW Presynaptic neurotoxin; Secreted; Signal; Toxin.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..50
FT /evidence="ECO:0000269|PubMed:10318957,
FT ECO:0000269|PubMed:10521453, ECO:0000269|PubMed:10679974"
FT /id="PRO_0000035016"
FT PEPTIDE 51..63
FT /note="Epsilon-conotoxin TxVA"
FT /evidence="ECO:0000269|PubMed:10318957,
FT ECO:0000269|PubMed:10521453, ECO:0000269|PubMed:10679974"
FT /id="PRO_0000035017"
FT PROPEP 64..67
FT /evidence="ECO:0000269|PubMed:10318957,
FT ECO:0000269|PubMed:10521453, ECO:0000269|PubMed:10679974"
FT /id="PRO_0000035018"
FT MOD_RES 51
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000269|PubMed:10318957,
FT ECO:0000269|PubMed:10521453"
FT MOD_RES 54
FT /note="4-carboxyglutamate"
FT /evidence="ECO:0000269|PubMed:10318957,
FT ECO:0000269|PubMed:10521453"
FT MOD_RES 57
FT /note="6'-bromotryptophan"
FT /evidence="ECO:0000269|PubMed:10318957,
FT ECO:0000269|PubMed:10521453"
FT MOD_RES 63
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:10318957,
FT ECO:0000269|PubMed:10521453"
FT CARBOHYD 60
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000269|PubMed:10318957,
FT ECO:0000269|PubMed:10521453"
FT DISULFID 52..58
FT /evidence="ECO:0000269|PubMed:10318957"
FT DISULFID 53..59
FT /evidence="ECO:0000269|PubMed:10318957"
FT TURN 53..56
FT /evidence="ECO:0007829|PDB:1WCT"
SQ SEQUENCE 67 AA; 7587 MW; 7270505504D6BB3D CRC64;
MRCFPVFIIL LLLIASAPCF DARTKTDDDV PLSSLRDNLK RTIRTRLNIR ECCEDGWCCT
AAPLTGR
