ID   CT5A_CONSP              Reviewed;          59 AA.
AC   P84845; C0KYC0; C0KYC1; C0KYC2;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 2.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=Conotoxin sr5a;
DE   AltName: Full=Sr5.1;
DE   AltName: Full=Sr5.2;
DE   AltName: Full=Sr5.3;
DE   Flags: Precursor;
OS   Conus spurius (Alphabet cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Lindaconus.
OX   NCBI_TaxID=192919;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SR5A AND SR5.2).
RC   TISSUE=Venom duct;
RX   PubMed=19447151; DOI=10.1016/j.peptides.2009.05.003;
RA   Zamora-Bustillos R., Aguilar M.B., Falcon A., Heimer de la Cotera E.P.;
RT   "Identification, by RT-PCR, of four novel T-1-superfamily conotoxins from
RT   the vermivorous snail Conus spurius from the Gulf of Mexico.";
RL   Peptides 30:1396-1404(2009).
RN   [2]
RP   PROTEIN SEQUENCE OF 47-59, FUNCTION, SUBCELLULAR LOCATION, MASS
RP   SPECTROMETRY, AND DISULFIDE BONDS.
RC   TISSUE=Venom;
RX   PubMed=16297502; DOI=10.1016/j.peptides.2005.07.020;
RA   Aguilar M.B., Lezama-Monfil L., Maillo M., Pedraza-Lara H., Lopez-Vera E.,
RA   Heimer de la Cotera E.P.;
RT   "A biologically active hydrophobic T-1-conotoxin from the venom of Conus
RT   spurius.";
RL   Peptides 27:500-505(2006).
CC   -!- FUNCTION: When injected intracranially into 9-day-old mice, causes
CC       transient symptoms, including extension of the body and clockwise and
CC       counter-clockwise turns, that last 3 to 4 minutes. When injected
CC       intracranially into 16-day-old mice, causes transient symptoms,
CC       including agitated breathing and occasional turning followed by
CC       scratching and grooming behavior, that last for 15-19 minutes.
CC       {ECO:0000269|PubMed:16297502}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16297502}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=sr5a; Synonyms=Sr5.1;
CC         IsoId=P84845-1; Sequence=Displayed;
CC       Name=Sr5.2;
CC         IsoId=P84845-2; Sequence=VSP_038848;
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:16297502}.
CC   -!- DOMAIN: The cysteine framework is V (CC-CC). {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=1616.6; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:16297502};
CC   -!- MASS SPECTROMETRY: Mass=1616.42; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16297502};
CC   -!- SIMILARITY: Belongs to the conotoxin T superfamily.
CC       {ECO:0000269|PubMed:16297502}.
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DR   EMBL; FJ646602; ACN22840.1; -; mRNA.
DR   EMBL; FJ646603; ACN22841.1; -; mRNA.
DR   EMBL; FJ646604; ACN22842.1; -; mRNA.
DR   AlphaFoldDB; P84845; -.
DR   ConoServer; 1618; SrVA.
DR   ConoServer; 3699; SrVA precursor.
DR   ConoServer; 3700; SrVA precursor.
DR   ConoServer; 3782; SrVA precursor.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0035821; P:modulation of process of another organism; IDA:UniProtKB.
DR   InterPro; IPR031565; T-conotoxin.
DR   Pfam; PF16981; Chi-conotoxin; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Disulfide bond; Neurotoxin; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..44
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000392714"
FT   PEPTIDE         47..59
FT                   /note="Conotoxin sr5a"
FT                   /evidence="ECO:0000269|PubMed:16297502"
FT                   /id="PRO_0000235846"
FT   DISULFID        51..58
FT                   /evidence="ECO:0000269|PubMed:16297502"
FT   DISULFID        52..59
FT                   /evidence="ECO:0000269|PubMed:16297502"
FT   VAR_SEQ         22..36
FT                   /note="Missing (in isoform Sr5.2)"
FT                   /evidence="ECO:0000303|PubMed:19447151"
FT                   /id="VSP_038848"
FT   VARIANT         7
FT                   /note="F -> L (in Sr5.3)"
SQ   SEQUENCE   59 AA;  6663 MW;  F309EAF6F98DB068 CRC64;
     MRCLPVFVIL LLLIASAPSV DAQLKTKDDV PLASFHDNAK GTQHKRIINW CCLIFYQCC