ID   CT5A_CONVR              Reviewed;          59 AA.
AC   P0C2B0; B4XT50;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 2.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Conotoxin ViVA {ECO:0000303|PubMed:16574176};
DE   AltName: Full=Vi1359 {ECO:0000303|PubMed:17544293};
DE   AltName: Full=Vi5.3 {ECO:0000303|PubMed:17933431};
DE   Flags: Precursor;
OS   Conus virgo (Virgin cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Virgiconus.
OX   NCBI_TaxID=89427;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom duct;
RX   PubMed=17933431; DOI=10.1016/j.peptides.2007.08.026;
RA   Peng C., Wu X., Han Y., Yuan D., Chi C., Wang C.;
RT   "Identification of six novel T-1 conotoxins from Conus pulicarius by
RT   molecular cloning.";
RL   Peptides 28:2116-2124(2007).
RN   [2]
RP   SYNTHESIS OF 47-58.
RX   PubMed=23567999; DOI=10.1016/j.bcp.2013.03.019;
RA   Petrel C., Hocking H.G., Reynaud M., Upert G., Favreau P., Biass D.,
RA   Paolini-Bertrand M., Peigneur S., Tytgat J., Gilles N., Hartley O.,
RA   Boelens R., Stocklin R., Servent D.;
RT   "Identification, structural and pharmacological characterization of tau-
RT   CnVA, a conopeptide that selectively interacts with somatostatin sst
RT   receptor.";
RL   Biochem. Pharmacol. 85:1663-1671(2013).
RN   [3]
RP   PROTEIN SEQUENCE OF 47-58, PYROGLUTAMATE FORMATION AT GLN-47, AMIDATION AT
RP   ILE-58, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=16574176; DOI=10.1016/j.toxicon.2006.01.023;
RA   Quinton L., Le Caer J.-P., Vinh J., Gilles N., Chamot-Rooke J.;
RT   "Fourier transform mass spectrometry: a powerful tool for toxin analysis.";
RL   Toxicon 47:715-726(2006).
RN   [4]
RP   PROTEIN SEQUENCE OF 47-58, PYROGLUTAMATE FORMATION AT GLN-47, AMIDATION AT
RP   ILE-58, DISULFIDE BONDS, AND MASS SPECTROMETRY.
RX   PubMed=17544293; DOI=10.1016/j.jasms.2007.04.009;
RA   Mandal A.K., Ramasamy M.R.S., Sabareesh V., Openshaw M.E., Krishnan K.S.,
RA   Balaram P.;
RT   "Sequencing of T-superfamily conotoxins from Conus virgo: pyroglutamic acid
RT   identification and disulfide arrangement by MALDI mass spectrometry.";
RL   J. Am. Soc. Mass Spectrom. 18:1396-1404(2007).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16574176}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:16574176}.
CC   -!- DOMAIN: The cysteine framework is V (CC-CC). {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=1358.6; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16574176};
CC   -!- MASS SPECTROMETRY: Mass=1358.6; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:17544293};
CC   -!- MISCELLANEOUS: Does not have the ability to interact with the G-protein
CC       coupled somatostatin type 3 receptor (SSTR3).
CC       {ECO:0000305|PubMed:23567999}.
CC   -!- SIMILARITY: Belongs to the conotoxin T superfamily. {ECO:0000305}.
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DR   EMBL; EU090178; ABW77586.1; -; mRNA.
DR   AlphaFoldDB; P0C2B0; -.
DR   ConoServer; 2833; ViVA precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR031565; T-conotoxin.
DR   Pfam; PF16981; Chi-conotoxin; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond;
KW   Pyrrolidone carboxylic acid; Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..46
FT                   /evidence="ECO:0000269|PubMed:16574176,
FT                   ECO:0000269|PubMed:17544293"
FT                   /id="PRO_0000316911"
FT   PEPTIDE         47..58
FT                   /note="Conotoxin ViVA"
FT                   /id="PRO_0000271475"
FT   MOD_RES         47
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:16574176,
FT                   ECO:0000269|PubMed:17544293"
FT   MOD_RES         58
FT                   /note="Isoleucine amide"
FT                   /evidence="ECO:0000269|PubMed:16574176,
FT                   ECO:0000269|PubMed:17544293"
FT   DISULFID        48..55
FT                   /evidence="ECO:0000269|PubMed:17544293"
FT   DISULFID        49..56
FT                   /evidence="ECO:0000269|PubMed:17544293"
SQ   SEQUENCE   59 AA;  6393 MW;  01BB16D5BE6F2FD4 CRC64;
     MRCVPVFIIL LLLIPSASSA AVQPKTEKDD VPLASVHDSA LRILSRQCCI TIPECCRIG