ID   CT5D_CONTE              Reviewed;          15 AA.
AC   P86264;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   29-SEP-2021, entry version 21.
DE   RecName: Full=Conotoxin tx5d {ECO:0000303|PubMed:23031820};
DE   AltName: Full=Trixin;
OS   Conus textile (Cloth-of-gold cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Cylinder.
OX   NCBI_TaxID=6494;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND AMIDATION AT
RP   THR-15.
RC   TISSUE=Venom;
RX   PubMed=19380747; DOI=10.1073/pnas.0900745106;
RA   Ueberheide B.M., Fenyo D., Alewood P.F., Chait B.T.;
RT   "Rapid sensitive analysis of cysteine rich peptide venom components.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:6910-6915(2009).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND AMIDATION AT
RP   THR-15.
RC   TISSUE=Venom;
RX   PubMed=22709442; DOI=10.1021/pr300312h;
RA   Bhatia S., Kil Y.J., Ueberheide B., Chait B.T., Tayo L., Cruz L., Lu B.,
RA   Yates J.R. III, Bern M.;
RT   "Constrained de novo sequencing of conotoxins.";
RL   J. Proteome Res. 11:4191-4200(2012).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, POSITION IN VENOM
RP   DUCT, AND AMIDATION AT THR-15.
RC   TISSUE=Venom;
RX   PubMed=23031820; DOI=10.1016/j.toxicon.2012.09.013;
RA   Dobson R., Collodoro M., Gilles N., Turtoi A., De Pauw E., Quinton L.;
RT   "Secretion and maturation of conotoxins in the venom ducts of Conus
RT   textile.";
RL   Toxicon 60:1370-1379(2012).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19380747}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct (PubMed:19380747,
CC       PubMed:23031820). Is mostly present in part 5 of the venom duct (distal
CC       part near the pharynx), and less abundantly present in part 4 of the
CC       venom duct (PubMed:23031820). {ECO:0000305|PubMed:19380747,
CC       ECO:0000305|PubMed:23031820}.
CC   -!- DOMAIN: The cysteine framework is V (CC-CC). {ECO:0000305}.
CC   -!- PTM: Contains 2 disulfide bonds. {ECO:0000269|PubMed:19380747}.
CC   -!- PTM: Contains 2 disulfide bonds that can be either 'C1-C3, C2-C4' or
CC       'C1-C4, C2-C3', since these disulfide connectivities have been observed
CC       for conotoxins with cysteine framework V (for examples, see AC P0DQQ7
CC       and AC P81755)... {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=1641.721; Mass_error=0.02; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:19380747};
CC   -!- SIMILARITY: Belongs to the conotoxin T superfamily. {ECO:0000305}.
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DR   ConoServer; 3758; Tx5d.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond; Neurotoxin; Secreted;
KW   Toxin.
FT   PEPTIDE         1..15
FT                   /note="Conotoxin tx5d"
FT                   /evidence="ECO:0000269|PubMed:19380747"
FT                   /id="PRO_0000371269"
FT   MOD_RES         15
FT                   /note="Threonine amide"
FT                   /evidence="ECO:0000269|PubMed:19380747,
FT                   ECO:0000269|PubMed:22709442, ECO:0000269|PubMed:23031820"
FT   UNSURE          2
FT                   /note="I or L"
FT                   /evidence="ECO:0000305|PubMed:19380747"
FT   UNSURE          4
FT                   /note="I or L"
FT                   /evidence="ECO:0000305|PubMed:19380747"
FT   UNSURE          5
FT                   /note="I or L"
FT                   /evidence="ECO:0000305|PubMed:19380747"
SQ   SEQUENCE   15 AA;  1648 MW;  54736BCAAE7ED015 CRC64;
     NIQIICCKHT PACCT