CTA1_NIACI
ID CTA1_NIACI Reviewed; 972 AA.
AC P94286;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Cycloisomaltooligosaccharide glucanotransferase;
DE Short=CITase;
DE EC=2.4.1.248;
DE Flags: Precursor;
OS Niallia circulans (Bacillus circulans).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Niallia.
OX NCBI_TaxID=1397;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=T-3040;
RA Oguma T., Kurokawa T., Tobe K., Kobayashi M.;
RT "Cloning and sequence analysis of the cycloisomaltooligosaccharide
RT glucanotransferase gene from Bacillus circulans T-3040 and expression in
RT Escherichia coli cells.";
RL J. Appl. Glycosci. 42:415-419(1995).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, AND PH DEPENDENCE.
RC STRAIN=T-3040;
RX PubMed=7515357; DOI=10.1016/0014-5793(94)00418-8;
RA Oguma T., Tobe K., Kobayashi M.;
RT "Purification and properties of a novel enzyme from Bacillus spp. T-3040,
RT which catalyzes the conversion of dextran to cyclic
RT isomaltooligosaccharides.";
RL FEBS Lett. 345:135-138(1994).
RN [3]
RP ERRATUM OF PUBMED:7515357.
RA Oguma T., Tobe K., Kobayashi M.;
RL FEBS Lett. 349:442-442(1994).
RN [4]
RP MUTAGENESIS OF ASP-183 AND ASP-308, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=T-3040;
RX PubMed=16926507; DOI=10.1271/bbb.60105;
RA Yamamoto T., Terasawa K., Kim Y.M., Kimura A., Kitamura Y., Kobayashi M.,
RA Funane K.;
RT "Identification of catalytic amino acids of cyclodextran glucanotransferase
RT from Bacillus circulans T-3040.";
RL Biosci. Biotechnol. Biochem. 70:1947-1953(2006).
CC -!- FUNCTION: Produces cycloisomaltooligosaccharide from dextran containing
CC 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-
CC glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans,
CC such as amylose. It also has no activity on oligosaccharides, such as
CC amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages
CC at branch points. {ECO:0000269|PubMed:7515357}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyclizes part of a (1->6)-alpha-D-glucan chain by formation of
CC a (1->6)-alpha-D-glucosidic bond.; EC=2.4.1.248;
CC Evidence={ECO:0000269|PubMed:7515357};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.063 mM for dextran T-40 {ECO:0000269|PubMed:16926507};
CC Vmax=0.872 pmol/min/ug enzyme with dextran T-40 as substrate
CC {ECO:0000269|PubMed:16926507};
CC pH dependence:
CC Optimum pH is 5.5. {ECO:0000269|PubMed:16926507,
CC ECO:0000269|PubMed:7515357};
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:7515357}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 66 family. {ECO:0000305}.
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DR EMBL; D61382; BAA09604.1; -; Genomic_DNA.
DR PDB; 3WNK; X-ray; 2.30 A; A=39-738.
DR PDB; 3WNL; X-ray; 2.60 A; A=39-738.
DR PDB; 3WNM; X-ray; 2.25 A; A=39-738.
DR PDB; 3WNN; X-ray; 2.25 A; A/B=39-738.
DR PDB; 3WNO; X-ray; 1.90 A; A/B=39-738.
DR PDB; 3WNP; X-ray; 2.80 A; A/B=39-738.
DR PDBsum; 3WNK; -.
DR PDBsum; 3WNL; -.
DR PDBsum; 3WNM; -.
DR PDBsum; 3WNN; -.
DR PDBsum; 3WNO; -.
DR PDBsum; 3WNP; -.
DR AlphaFoldDB; P94286; -.
DR SMR; P94286; -.
DR CAZy; CBM35; Carbohydrate-Binding Module Family 35.
DR CAZy; GH66; Glycoside Hydrolase Family 66.
DR KEGG; ag:BAA09604; -.
DR BRENDA; 2.4.1.248; 649.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR CDD; cd14745; GH66; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 2.60.40.1180; -; 1.
DR InterPro; IPR005084; CMB_fam6.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR025092; Glyco_hydro_66.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR013783; Ig-like_fold.
DR Pfam; PF16990; CBM_35; 1.
DR Pfam; PF03422; CBM_6; 1.
DR Pfam; PF13199; Glyco_hydro_66; 1.
DR SUPFAM; SSF49785; SSF49785; 2.
DR PROSITE; PS51175; CBM6; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Glycosyltransferase; Repeat; Signal; Transferase.
FT SIGNAL 1..38
FT /evidence="ECO:0000255"
FT CHAIN 39..972
FT /note="Cycloisomaltooligosaccharide glucanotransferase"
FT /id="PRO_0000012241"
FT DOMAIN 421..546
FT /note="CBM6 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00523"
FT DOMAIN 748..871
FT /note="CBM6 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00523"
FT MUTAGEN 183
FT /note="D->N: Has 1% of wild-type activity."
FT /evidence="ECO:0000269|PubMed:16926507"
FT MUTAGEN 308
FT /note="D->N: No activity."
FT /evidence="ECO:0000269|PubMed:16926507"
FT STRAND 44..50
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:3WNN"
FT STRAND 59..67
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 70..72
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 74..84
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 87..98
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 103..111
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 114..125
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 127..129
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 131..138
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 142..144
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 148..151
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 160..174
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 178..181
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 185..187
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 201..204
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 210..212
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 213..225
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 229..242
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 244..246
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 250..252
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 253..257
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 265..267
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 269..272
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 274..278
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 283..300
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 303..308
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 315..318
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 326..328
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 330..344
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 346..348
FT /evidence="ECO:0007829|PDB:3WNP"
FT STRAND 350..357
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 365..371
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 375..380
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 383..385
FT /evidence="ECO:0007829|PDB:3WNM"
FT HELIX 388..401
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 407..410
FT /evidence="ECO:0007829|PDB:3WNO"
FT TURN 413..416
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 420..424
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 425..427
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 428..432
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 434..436
FT /evidence="ECO:0007829|PDB:3WNN"
FT STRAND 439..441
FT /evidence="ECO:0007829|PDB:3WNP"
FT STRAND 447..451
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 457..477
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 480..482
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 484..490
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 493..500
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 504..507
FT /evidence="ECO:0007829|PDB:3WNP"
FT STRAND 509..519
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 521..529
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 538..547
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 550..562
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 566..568
FT /evidence="ECO:0007829|PDB:3WNO"
FT TURN 572..574
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 579..581
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 586..588
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 591..606
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 608..612
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 621..623
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 626..628
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 633..636
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 641..648
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 650..659
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 665..669
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 677..686
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 692..698
FT /evidence="ECO:0007829|PDB:3WNO"
FT HELIX 703..705
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 708..710
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 713..717
FT /evidence="ECO:0007829|PDB:3WNO"
FT STRAND 720..738
FT /evidence="ECO:0007829|PDB:3WNO"
SQ SEQUENCE 972 AA; 107431 MW; 2827BEA61782CC22 CRC64;
MVRFMYALRK RRLSLLLAMS LLVMCVASVV SPPPQALASG SGGIERVFTD KARYNPGDAV
SIRVQAKNGT GSSWSGAARL EIFHLENSVY TSSQSLSLTN GQSTTLTFTW TAPSTDFRGY
FVRIDAGTLG QGATAIDVSS DFTKYPRYGY ISEFESGETA LESKAKVDQL AQDYHINAWQ
FYDWMWRHDK MIKRTGGSID STWLDLFNRE ISWSTLQNQI DAVHDVNGKA MAYAMIYASR
ENYSPLGISP TWGIYEDSSH TNQFDVDFGD GSTYLYMSDP QNPNWQNYIH AEYIDSINTA
GFDGIHVDQM GQRSNVYDYN GNSIDLSTRF SPFLDQAKSV LSANNPARDN LTYNIVDGTV
NGWAVNDVSK NADLDFLYSE IWYLSDSYNQ LKNYIEQLRA NGGNKAVVLA AYMNYADNAG
TRYEAESASM TNVSTNTNHA GYTGSGFVDQ FASTGDKVSF AINAPEAGDY SLVFRYGNNT
GANSTLNLYV DGNFVQKLYF FNQSSWGTWK HDAWYQVPLT QGAHTVELRY ESGNVGAVNL
DSLTLGTFDE HSVRLADAMM SASGATHIEL GDDNQMLPHE YYPNRSKTMR SSLKNAMKDH
YNFITAYENL LFDSDVVPND TGSQFVNLTG VSASGDGSAN TVWYINKRTS DYNIVHLINL
LGNDNQWRNT ASQPSFQTNL PAKIYIGADE TISDVYLASP DLSGGETQEL AFTSGTDAGG
KYVSFTVPEL KYWNMIYMKR TFSVPANDIY EAETAIKSNV STNTNHAGYT GSGFVDGFSS
TNDGVSFVVK STASDDYALR FRYANGGSDA TRDVYVDGKL AGTVSFKSTG SWSTWSYGEI
TARLEPGHHT IVLWQTSGNT GAINLDHLDL DKTYIWQFDR QIVSVPAGYR ITFRTGLPGW
VHWGVNGWTG VTDTPLRSNG SLDGNLDHET SIGPFATGTA VDVTFLWDDN NNGILEPSTD
RWEGTDFGIN VS
