ID   CTCN2_ECHES             Reviewed;         121 AA.
AC   A0A144LVL3;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   08-JUN-2016, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Centrocin 2 {ECO:0000312|EMBL:AMT92375.1};
DE   AltName: Full=EeCentrocin 2 {ECO:0000303|PubMed:27007817};
DE   Contains:
DE     RecName: Full=Centrocin 2, heavy chain {ECO:0000303|PubMed:27007817};
DE   Contains:
DE     RecName: Full=Centrocin 2, light chain {ECO:0000303|PubMed:27007817};
DE   Flags: Precursor;
OS   Echinus esculentus (Sea urchin).
OC   Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC   Euechinoidea; Echinacea; Camarodonta; Echinidea; Echinidae; Echinus.
OX   NCBI_TaxID=7648 {ECO:0000312|EMBL:AMT92375.1};
RN   [1] {ECO:0000312|EMBL:AMT92375.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-82 AND 105-117,
RP   FUNCTION, MASS SPECTROMETRY, BROMINATION AT TRP-51 AND TRP-59, AMIDATION AT
RP   HIS-119, AND PYROGLUTAMATE FORMATION AT GLN-107.
RC   TISSUE=Coelomocyte {ECO:0000303|PubMed:27007817};
RX   PubMed=27007817; DOI=10.1371/journal.pone.0151820;
RA   Solstad R.G., Li C., Isaksson J., Johansen J., Svenson J., Stensvag K.,
RA   Haug T.;
RT   "Novel Antimicrobial Peptides EeCentrocins 1, 2 and EeStrongylocin 2 from
RT   the Edible Sea Urchin Echinus esculentus Have 6-Br-Trp Post-Translational
RT   Modifications.";
RL   PLoS ONE 11:E0151820-E0151820(2016).
CC   -!- FUNCTION: [Centrocin 2, heavy chain]: Has antimicrobial activity
CC       against Gram-negative bacteria, Gram-positive bacteria and against
CC       fungi with minimum inhibitory concentration (MIC) between 0.78 uM and
CC       50 uM. Shows little hemolytic activity at concentrations up to 12.5 uM
CC       but >50% lysis at 100 uM. {ECO:0000269|PubMed:27007817}.
CC   -!- SUBUNIT: Heterodimer of a light and a heavy chain, probably disulfide-
CC       linked. {ECO:0000269|PubMed:27007817}.
CC   -!- MASS SPECTROMETRY: Mass=5019.37; Method=Electrospray; Note=The measured
CC       ranges are 51-82, 107-119.; Evidence={ECO:0000269|PubMed:27007817};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; KR494263; AMT92375.1; -; mRNA.
DR   AlphaFoldDB; A0A144LVL3; -.
DR   GO; GO:0046982; F:protein heterodimerization activity; IPI:UniProtKB.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0044179; P:hemolysis in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Bromination; Cytolysis;
KW   Direct protein sequencing; Disulfide bond; Fungicide; Hemolysis;
KW   Pyrrolidone carboxylic acid; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..50
FT                   /evidence="ECO:0000305|PubMed:27007817"
FT                   /id="PRO_0000438842"
FT   PEPTIDE         51..82
FT                   /note="Centrocin 2, heavy chain"
FT                   /evidence="ECO:0000269|PubMed:27007817"
FT                   /id="PRO_0000438843"
FT   PROPEP          83..106
FT                   /evidence="ECO:0000305|PubMed:27007817"
FT                   /id="PRO_0000438844"
FT   PEPTIDE         107..119
FT                   /note="Centrocin 2, light chain"
FT                   /evidence="ECO:0000269|PubMed:27007817"
FT                   /id="PRO_0000438845"
FT   MOD_RES         51
FT                   /note="6'-bromotryptophan"
FT                   /evidence="ECO:0000269|PubMed:27007817"
FT   MOD_RES         59
FT                   /note="6'-bromotryptophan"
FT                   /evidence="ECO:0000269|PubMed:27007817"
FT   MOD_RES         107
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:27007817"
FT   MOD_RES         119
FT                   /note="Histidine amide"
FT                   /evidence="ECO:0000269|PubMed:27007817"
FT   DISULFID        77..112
FT                   /evidence="ECO:0000303|PubMed:27007817"
SQ   SEQUENCE   121 AA;  13151 MW;  E386FC153EFE6838 CRC64;
     MMIKIAVVLC AVMATSMVFA NDVKEQELAD LLDLLISEEV SSPDDAVAES WGHKLRSSWN
     KVKHAVKKGA GYASGACRVL GHSPQEARAK VLEAFPEMKE SDLDEEQVGK YCAVAHAIHG
     R