CTC_BACSU
ID CTC_BACSU Reviewed; 204 AA.
AC P14194;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=General stress protein CTC;
DE AltName: Full=50S ribosomal protein L25;
GN Name=ctc; OrderedLocusNames=BSU00520;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA Ogasawara N., Nakai S., Yoshikawa H.;
RT "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT chromosome containing the replication origin.";
RL DNA Res. 1:1-14(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-186.
RX PubMed=2555671; DOI=10.1007/bf00332425;
RA Nilsson D., Hove-Jensen B., Arnvig K.;
RT "Primary structure of the tms and prs genes of Bacillus subtilis.";
RL Mol. Gen. Genet. 218:565-571(1989).
RN [4]
RP PROTEIN SEQUENCE OF 2-14.
RC STRAIN=168 / IS58;
RX PubMed=8012595; DOI=10.1099/00221287-140-4-741;
RA Voelker U., Engelmann S., Maul B., Riethdorf S., Voelker A., Schmid R.,
RA Mach H., Hecker M.;
RT "Analysis of the induction of general stress proteins of Bacillus
RT subtilis.";
RL Microbiology 140:741-752(1994).
RN [5]
RP DISRUPTION PHENOTYPE.
RC STRAIN=168 / 1A3;
RX PubMed=2836704; DOI=10.1007/bf00322460;
RA Truitt C.L., Weaver E.A., Haldenwang W.G.;
RT "Effects on growth and sporulation of inactivation of a Bacillus subtilis
RT gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases
RT (E-sigma 37, E-sigma 32).";
RL Mol. Gen. Genet. 212:166-171(1988).
RN [6]
RP DETECTION IN RIBOSOMES, AND BINDING TO 5S RRNA.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=12432960;
RA Schmalisch M., Langbein I., Stuelke J.;
RT "The general stress protein Ctc of Bacillus subtilis is a ribosomal
RT protein.";
RL J. Mol. Microbiol. Biotechnol. 4:495-501(2002).
RN [7]
RP BINDING TO 5S RRNA.
RX PubMed=15236599; DOI=10.1023/b:biry.0000033733.60180.e3;
RA Korepanov A.P., Gongadze G.M., Garber M.B.;
RT "General stress protein CTC from Bacillus subtilis specifically binds to
RT ribosomal 5S RNA.";
RL Biochemistry (Mosc.) 69:607-611(2004).
CC -!- FUNCTION: Not required for exponential growth; probably functions in
CC vegetatively growing cells, maybe required for accurate translation
CC under stress conditions.
CC -!- SUBUNIT: Part of the ribosome (presumably the 50S subunit) under heat-
CC stress but not control growth conditions. Binds 5S rRNA.
CC -!- DEVELOPMENTAL STAGE: Expressed at the end of exponential growth under
CC conditions in which the enzymes of the TCA cycle are repressed.
CC -!- INDUCTION: By heat shock, salt stress, oxidative stress, glucose
CC limitation and oxygen limitation.
CC -!- DISRUPTION PHENOTYPE: Cells sporulate poorly at 48 degrees Celsius.
CC {ECO:0000269|PubMed:2836704}.
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL25 family. CTC
CC subfamily. {ECO:0000305}.
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DR EMBL; D26185; BAA05287.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11828.1; -; Genomic_DNA.
DR EMBL; X16518; CAA34524.1; -; Genomic_DNA.
DR PIR; S66082; S66082.
DR RefSeq; NP_387933.1; NC_000964.3.
DR RefSeq; WP_003243443.1; NZ_JNCM01000028.1.
DR AlphaFoldDB; P14194; -.
DR SMR; P14194; -.
DR STRING; 224308.BSU00520; -.
DR jPOST; P14194; -.
DR PaxDb; P14194; -.
DR PRIDE; P14194; -.
DR EnsemblBacteria; CAB11828; CAB11828; BSU_00520.
DR GeneID; 936984; -.
DR KEGG; bsu:BSU00520; -.
DR PATRIC; fig|224308.179.peg.52; -.
DR eggNOG; COG1825; Bacteria.
DR InParanoid; P14194; -.
DR OMA; HVDFYEV; -.
DR PhylomeDB; P14194; -.
DR BioCyc; BSUB:BSU00520-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0008097; F:5S rRNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd00495; Ribosomal_L25_TL5_CTC; 1.
DR Gene3D; 2.170.120.20; -; 1.
DR Gene3D; 2.40.240.10; -; 1.
DR HAMAP; MF_01334; Ribosomal_L25_CTC; 1.
DR InterPro; IPR020056; Rbsml_L25/Gln-tRNA_synth_N.
DR InterPro; IPR029751; Ribosomal_L25.
DR InterPro; IPR011035; Ribosomal_L25/Gln-tRNA_synth.
DR InterPro; IPR020057; Ribosomal_L25_b-dom.
DR InterPro; IPR037121; Ribosomal_L25_C.
DR InterPro; IPR001021; Ribosomal_L25_long.
DR Pfam; PF01386; Ribosomal_L25p; 1.
DR Pfam; PF14693; Ribosomal_TL5_C; 1.
DR SUPFAM; SSF50715; SSF50715; 1.
DR TIGRFAMs; TIGR00731; bL25_bact_ctc; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; RNA-binding; rRNA-binding; Stress response.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:8012595"
FT CHAIN 2..204
FT /note="General stress protein CTC"
FT /id="PRO_0000181512"
FT REGION 177..204
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..204
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 2
FT /note="A -> R (in strain: IS58)"
FT VARIANT 10
FT /note="T -> Q (in strain: IS58)"
FT VARIANT 13
FT /note="T -> I (in strain: IS58)"
SQ SEQUENCE 204 AA; 22056 MW; D72CEA9437C094E7 CRC64;
MATLTAKERT DFTRSSLRNI RTSGHVPGII YGKDTGNKPV SLDSVELIKT LRDEGKNAVI
TLEVSGEKHS VMVTDLQTDP LKNEITHADF QVVNMSEDIE VEVPIHLTGE AIGVKNGGVL
QQPLYALTVK AKPKAIPQTI EADISSLDVN EVLTIADLPA GGDYSFNHES DEVVASILPP
QQQEAAEVDE EESADAQPEG ENEQ
