CTH1_YEAST
ID CTH1_YEAST Reviewed; 325 AA.
AC P47976; D6VSD3;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=mRNA decay factor CTH1;
DE AltName: Full=Cysteine-three-histidine protein 1;
GN Name=CTH1; OrderedLocusNames=YDR151C; ORFNames=YD8358.07C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8890739; DOI=10.1016/0378-1119(96)00084-4;
RA Thompson M.J., Lai W.S., Taylor G.A., Blackshear P.J.;
RT "Cloning and characterization of two yeast genes encoding members of the
RT CCCH class of zinc finger proteins: zinc finger-mediated impairment of cell
RT growth.";
RL Gene 174:225-233(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION, INDUCTION, MUTAGENESIS OF CYS-225, AND MRNA-BINDING.
RX PubMed=18522836; DOI=10.1016/j.cmet.2008.04.010;
RA Puig S., Vergara S.V., Thiele D.J.;
RT "Cooperation of two mRNA-binding proteins drives metabolic adaptation to
RT iron deficiency.";
RL Cell Metab. 7:555-564(2008).
CC -!- FUNCTION: Binds to specific AU-rich elements (ARE) in the 3'-
CC untranslated region of target mRNAs and promotes their degradation. In
CC response to iron deficiency, promotes the decay of many mRNAs encoding
CC proteins involved in iron-dependent pathways. Negatively regulates
CC primarily iron-dependent mitochondrial processes including respiration
CC and amino acid biosynthesis. {ECO:0000269|PubMed:18522836}.
CC -!- INDUCTION: By transcription factors AFT1 and AFT2 in response to iron
CC deficiency. {ECO:0000269|PubMed:18522836}.
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DR EMBL; L42133; AAB39897.1; -; Genomic_DNA.
DR EMBL; Z50046; CAA90373.1; -; Genomic_DNA.
DR EMBL; AY557690; AAS56016.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11993.1; -; Genomic_DNA.
DR PIR; S57977; S57977.
DR RefSeq; NP_010435.1; NM_001180458.1.
DR AlphaFoldDB; P47976; -.
DR SMR; P47976; -.
DR BioGRID; 32204; 60.
DR DIP; DIP-1784N; -.
DR IntAct; P47976; 5.
DR MINT; P47976; -.
DR STRING; 4932.YDR151C; -.
DR iPTMnet; P47976; -.
DR PaxDb; P47976; -.
DR PRIDE; P47976; -.
DR EnsemblFungi; YDR151C_mRNA; YDR151C; YDR151C.
DR GeneID; 851729; -.
DR KEGG; sce:YDR151C; -.
DR SGD; S000002558; CTH1.
DR VEuPathDB; FungiDB:YDR151C; -.
DR eggNOG; KOG1677; Eukaryota.
DR GeneTree; ENSGT00940000170800; -.
DR HOGENOM; CLU_060370_0_0_1; -.
DR InParanoid; P47976; -.
DR OMA; KPCINWS; -.
DR BioCyc; YEAST:G3O-29745-MON; -.
DR Reactome; R-SCE-450385; Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.
DR Reactome; R-SCE-450513; Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA.
DR ChiTaRS; CTL1; yeast.
DR PRO; PR:P47976; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P47976; protein.
DR GO; GO:0005634; C:nucleus; IC:SGD.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; ISS:SGD.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IGI:SGD.
DR GO; GO:0000956; P:nuclear-transcribed mRNA catabolic process; IGI:SGD.
DR InterPro; IPR045877; ZFP36-like.
DR InterPro; IPR000571; Znf_CCCH.
DR InterPro; IPR036855; Znf_CCCH_sf.
DR PANTHER; PTHR12547; PTHR12547; 1.
DR Pfam; PF00642; zf-CCCH; 2.
DR SMART; SM00356; ZnF_C3H1; 2.
DR SUPFAM; SSF90229; SSF90229; 2.
DR PROSITE; PS50103; ZF_C3H1; 2.
PE 1: Evidence at protein level;
KW Metal-binding; Reference proteome; Repeat; RNA-binding; Zinc; Zinc-finger.
FT CHAIN 1..325
FT /note="mRNA decay factor CTH1"
FT /id="PRO_0000089173"
FT ZN_FING 204..232
FT /note="C3H1-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 242..270
FT /note="C3H1-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 284..306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 225
FT /note="C->R: Abolishes mRNA binding."
FT /evidence="ECO:0000269|PubMed:18522836"
FT CONFLICT 142..143
FT /note="EI -> RV (in Ref. 1; AAB39897)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 325 AA; 36772 MW; 8B5966F88CE096DE CRC64;
MMPNVAPNSY YLNIPNANST STTTSSIFSD LNKEYESKIK EIEEYYIKTL LNENTDNDDS
SSSEGHNINE TDILSEYSPR PSPWLPSKPN CYHPLGDFKD LIISDSRPTN TLPINNPFAG
NNNISTLATT EKKRKKRSLE VEINPTYTTS AFSLPLTAEN LQKLSQVDSQ STGLPYTLPI
QKTTKLEPCR RAPLQLPQLV NKTLYKTELC ESFTIKGYCK YGNKCQFAHG LNELKFKKKS
NNYRTKPCIN WSKLGYCPYG KRCCFKHGDD KDVEIYQNAN DGRSKDTALT PLPTSLAPSN
NDNITNLSKP RNLHTSVKAL QRMTW
