ID   CTHL1_BOVIN             Reviewed;         155 AA.
AC   P22226; A5PJA3; Q24JY5;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 2.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=Cathelicidin-1;
DE   AltName: Full=Bactenecin-1;
DE            Short=Bac1;
DE   AltName: Full=Cyclic dodecapeptide;
DE   Flags: Precursor;
GN   Name=CATHL1; Synonyms=BAC1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Bone marrow;
RX   PubMed=1459251; DOI=10.1016/0014-5793(92)80971-i;
RA   Storici P., del Sal G., Schneider C., Zanetti M.;
RT   "cDNA sequence analysis of an antibiotic dodecapeptide from neutrophils.";
RL   FEBS Lett. 314:187-190(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Liver;
RA   Scocchi M., Wang S., Zanetti M.;
RL   Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-128.
RC   STRAIN=Hereford; TISSUE=Thymus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 144-155.
RC   TISSUE=Neutrophil;
RX   PubMed=3290210; DOI=10.1016/s0021-9258(19)81553-3;
RA   Romeo D., Skerlavaj B., Bolognesi M., Gennaro R.;
RT   "Structure and bactericidal activity of an antibiotic dodecapeptide
RT   purified from bovine neutrophils.";
RL   J. Biol. Chem. 263:9573-9575(1988).
RN   [5]
RP   CHARACTERIZATION, AND PYROGLUTAMATE FORMATION AT GLN-30.
RX   PubMed=8706679; DOI=10.1111/j.1432-1033.1996.0769w.x;
RA   Storici P., Tossi A., Lenarcic B., Romeo D.;
RT   "Purification and structural characterization of bovine cathelicidins,
RT   precursors of antimicrobial peptides.";
RL   Eur. J. Biochem. 238:769-776(1996).
CC   -!- FUNCTION: Potent microbicidal activity; active against S.aureus and
CC       E.coli.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Large granules of neutrophils.
CC   -!- SIMILARITY: Belongs to the cathelicidin family. {ECO:0000305}.
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DR   EMBL; L08834; AAA50615.1; -; mRNA.
DR   EMBL; Y09472; CAA70617.1; -; Genomic_DNA.
DR   EMBL; BC114189; AAI14190.1; -; mRNA.
DR   EMBL; BC142024; AAI42025.1; -; mRNA.
DR   PIR; S27018; S27018.
DR   RefSeq; NP_777250.1; NM_174825.1.
DR   RefSeq; XP_015315138.1; XM_015459652.1.
DR   RefSeq; XP_015324278.1; XM_015468792.1.
DR   AlphaFoldDB; P22226; -.
DR   SMR; P22226; -.
DR   STRING; 9913.ENSBTAP00000026750; -.
DR   PaxDb; P22226; -.
DR   PeptideAtlas; P22226; -.
DR   PRIDE; P22226; -.
DR   Ensembl; ENSBTAT00000011364; ENSBTAP00000011364; ENSBTAG00000013356.
DR   Ensembl; ENSBTAT00000026750; ENSBTAP00000026750; ENSBTAG00000013356.
DR   Ensembl; ENSBTAT00000079324; ENSBTAP00000062320; ENSBTAG00000013356.
DR   Ensembl; ENSBTAT00000085052; ENSBTAP00000070159; ENSBTAG00000013356.
DR   GeneID; 282164; -.
DR   KEGG; bta:282164; -.
DR   CTD; 282164; -.
DR   VEuPathDB; HostDB:ENSBTAG00000013356; -.
DR   eggNOG; ENOG502SAES; Eukaryota.
DR   GeneTree; ENSGT00390000000410; -.
DR   HOGENOM; CLU_121724_1_1_1; -.
DR   InParanoid; P22226; -.
DR   OrthoDB; 1534863at2759; -.
DR   TreeFam; TF338457; -.
DR   Proteomes; UP000009136; Chromosome 22.
DR   Bgee; ENSBTAG00000013356; Expressed in thymus and 51 other tissues.
DR   ExpressionAtlas; P22226; differential.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0001530; F:lipopolysaccharide binding; IBA:GO_Central.
DR   GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR   GO; GO:0019835; P:cytolysis; IMP:AgBase.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:AgBase.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IMP:AgBase.
DR   GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR   InterPro; IPR001894; Cathelicidin-like.
DR   InterPro; IPR018216; Cathelicidin_CS.
DR   InterPro; IPR046350; Cystatin_sf.
DR   PANTHER; PTHR10206; PTHR10206; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00946; CATHELICIDINS_1; 1.
DR   PROSITE; PS00947; CATHELICIDINS_2; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   PROPEP          30..143
FT                   /evidence="ECO:0000269|PubMed:3290210"
FT                   /id="PRO_0000004696"
FT   PEPTIDE         144..155
FT                   /note="Cathelicidin-1"
FT                   /id="PRO_0000004697"
FT   MOD_RES         30
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:8706679"
FT   DISULFID        85..96
FT                   /evidence="ECO:0000250"
FT   DISULFID        107..124
FT                   /evidence="ECO:0000250"
FT   DISULFID        146..154
FT                   /evidence="ECO:0000269|PubMed:3290210"
FT   VARIANT         128
FT                   /note="Q -> R"
FT                   /evidence="ECO:0000269|Ref.3"
SQ   SEQUENCE   155 AA;  17600 MW;  008CD7DC6CB91BF9 CRC64;
     METPRASLSL GRWSLWLLLL GLALPSASAQ ALSYREAVLR AVDQLNEQSS EPNIYRLLEL
     DQPPQDDEDP DSPKRVSFRV KETVCSRTTQ QPPEQCDFKE NGLLKRCEGT VTLDQVRGNF
     DITCNNHQSI RITKQPWAPP QAARLCRIVV IRVCR