CTL1_SCHPO
ID CTL1_SCHPO Reviewed; 574 AA.
AC O74441;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Choline transporter-like protein ctl1;
GN Name=ctl1; ORFNames=SPCC1682.11c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, FUNCTION, AND INTERACTION WITH
RP ATG9.
RX PubMed=23950735; DOI=10.1371/journal.pgen.1003715;
RA Sun L.L., Li M., Suo F., Liu X.M., Shen E.Z., Yang B., Dong M.Q., He W.Z.,
RA Du L.L.;
RT "Global analysis of fission yeast mating genes reveals new autophagy
RT factors.";
RL PLoS Genet. 9:E1003715-E1003715(2013).
CC -!- FUNCTION: Required for the normal organization of the preautophagosomal
CC structure (PAS) and for the correct subcellular location of atg9.
CC {ECO:0000269|PubMed:23950735}.
CC -!- SUBUNIT: Interacts with atg9. {ECO:0000269|PubMed:23950735}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein. Preautophagosomal structure membrane; Multi-pass
CC membrane protein.
CC -!- DISRUPTION PHENOTYPE: Impairs atg8-processing.
CC {ECO:0000269|PubMed:23950735}.
CC -!- SIMILARITY: Belongs to the CTL (choline transporter-like) family.
CC {ECO:0000305}.
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DR EMBL; CU329672; CAA20677.1; -; Genomic_DNA.
DR PIR; T41068; T41068.
DR RefSeq; NP_587804.1; NM_001022797.2.
DR AlphaFoldDB; O74441; -.
DR SMR; O74441; -.
DR BioGRID; 275511; 23.
DR STRING; 4896.SPCC1682.11c.1; -.
DR PaxDb; O74441; -.
DR EnsemblFungi; SPCC1682.11c.1; SPCC1682.11c.1:pep; SPCC1682.11c.
DR GeneID; 2538935; -.
DR KEGG; spo:SPCC1682.11c; -.
DR PomBase; SPCC1682.11c; ctl1.
DR VEuPathDB; FungiDB:SPCC1682.11c; -.
DR eggNOG; KOG1362; Eukaryota.
DR HOGENOM; CLU_473404_0_0_1; -.
DR InParanoid; O74441; -.
DR OMA; WILMFTR; -.
DR PhylomeDB; O74441; -.
DR PRO; PR:O74441; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IDA:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0000407; C:phagophore assembly site; IDA:PomBase.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015220; F:choline transmembrane transporter activity; ISS:PomBase.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0016236; P:macroautophagy; IMP:PomBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR InterPro; IPR007603; Choline_transptr-like.
DR PANTHER; PTHR12385; PTHR12385; 1.
DR Pfam; PF04515; Choline_transpo; 1.
PE 1: Evidence at protein level;
KW Autophagy; Endoplasmic reticulum; Glycoprotein; Membrane;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..574
FT /note="Choline transporter-like protein ctl1"
FT /id="PRO_0000350953"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..456
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 485..505
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 511..531
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 40
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 101
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 457
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 558
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 574 AA; 65523 MW; C7889326CFE9E0E3 CRC64;
MFSDYASRFL AQSRFSSVDQ RNFKYPTSRS NLQSVSIDRN SSIDSHETDL SAGSSSLHGL
NSLIDSGSIH WQLREQEQSN SHISRNENEL FSKENSIYNG NFSENLGVQP NPQTISHESV
NEEYTELPYD AHLPSEQKVP DRKWGLTFGF LTLALFTYSF LMVWRTNPNI PPATSPYAAI
QKAFPLFHKD AIICMMLSVI WLFCLVAIPR FLYFLLASVP LTMFAFAVYL LKASRIHLET
SIQPKLMLLT GIILLVAPIL LSYYVWRRRI HFETSFNIIR LACRVIADIP QITLIFISFL
FSFYVLIFIW VRLFARLFLR GSTLVGSVWV LPRSSWVLAS FYSLHFLWLC TFFHALQCAI
ISSIVSQWFF YRDTKSSATK TNLVSHFFYH VVSNQYGLCA FSSFLVVITK VPLHFLPTWL
RHVSRIVYYM FSKTSASYVT SPLTLAYASI YSVPYMNASK ALYQIEQLNR VGLRRRSYYF
SKYTLLAARS LLAIGVGVTS WNYSIHENGV FYGYIVGLLG GFLAWLIIGA IEGGLSMIVD
ALLICSIIDI SSCQGDPNGS HCFEAWQLFE SNGY
