CTL2_CAVPO
ID CTL2_CAVPO Reviewed; 705 AA.
AC Q810F1;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Choline transporter-like protein 2;
DE AltName: Full=Inner ear supporting cell antigen;
DE Short=IESCA;
DE AltName: Full=Solute carrier family 44 member 2;
GN Name=SLC44A2; Synonyms=CTL2;
OS Cavia porcellus (Guinea pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae;
OC Cavia.
OX NCBI_TaxID=10141;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, TISSUE
RP SPECIFICITY, GLYCOSYLATION, SUBCELLULAR LOCATION, AND FUNCTION.
RC TISSUE=Inner ear;
RX PubMed=14973250; DOI=10.1523/jneurosci.5063-03.2004;
RA Nair T.S., Kozma K.E., Hoefling N.L., Kommareddi P.K., Ueda Y., Gong T.-W.,
RA Lomax M.I., Lansford C.D., Telian S.A., Satar B., Arts H.A.,
RA El-Kashlan H.K., Berryhill W.E., Raphael Y., Carey T.E.;
RT "Identification and characterization of choline transporter-like protein 2,
RT an inner ear glycoprotein of 68 and 72 kDa that is the target of antibody-
RT induced hearing loss.";
RL J. Neurosci. 24:1772-1779(2004).
RN [2]
RP TISSUE SPECIFICITY, AND GLYCOSYLATION.
RX PubMed=20665236; DOI=10.1007/s10930-010-9268-y;
RA Kommareddi P.K., Nair T.S., Thang L.V., Galano M.M., Babu E., Ganapathy V.,
RA Kanazawa T., McHugh J.B., Carey T.E.;
RT "Isoforms, expression, glycosylation, and tissue distribution of
RT CTL2/SLC44A2.";
RL Protein J. 29:417-426(2010).
CC -!- FUNCTION: Choline transporter (By similarity). Essential for hair cell
CC survival. {ECO:0000250, ECO:0000269|PubMed:14973250}.
CC -!- SUBUNIT: Interacts with COCH. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:14973250}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:14973250}.
CC -!- TISSUE SPECIFICITY: Expressed at high levels in lung, colon and in
CC supporting cells of the inner ear (at protein level). Progressively
CC lower levels in brain, tongue, liver and kidney (at protein level). In
CC the tongue, strongly expressed in epithelial cells and in nerves within
CC the musculature. Within the nerves, expression observed in the
CC perineurial cells of the nerve sheath, in the Schwann cells and
CC myelinated nerve fibers (at protein level). In the kidney, prominent
CC expression in glomeruli in the lining of Bowman's capsule and on the
CC mesangial cells adjacent to the vessels within the glomerulus (at
CC protein level). Strongly expressed on the membranes of splenocytes (at
CC protein level). {ECO:0000269|PubMed:14973250,
CC ECO:0000269|PubMed:20665236}.
CC -!- PTM: N-glycosylated; contains sialic acid. Not O-glycosylated.
CC {ECO:0000269|PubMed:14973250, ECO:0000269|PubMed:20665236}.
CC -!- MISCELLANEOUS: Antibody KHRI-3, which is directed against a N-linked
CC carbohydrate of CTL2, causes hearing loss when infused in the organ of
CC Corti.
CC -!- SIMILARITY: Belongs to the CTL (choline transporter-like) family.
CC {ECO:0000305}.
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DR EMBL; AY233002; AAO74601.1; -; mRNA.
DR RefSeq; NP_001166487.1; NM_001173016.1.
DR AlphaFoldDB; Q810F1; -.
DR SMR; Q810F1; -.
DR STRING; 10141.ENSCPOP00000012358; -.
DR GeneID; 100135617; -.
DR KEGG; cpoc:100135617; -.
DR CTD; 57153; -.
DR eggNOG; KOG1362; Eukaryota.
DR InParanoid; Q810F1; -.
DR Proteomes; UP000005447; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015101; F:organic cation transmembrane transporter activity; IEA:UniProt.
DR GO; GO:0071705; P:nitrogen compound transport; IEA:UniProt.
DR InterPro; IPR007603; Choline_transptr-like.
DR PANTHER; PTHR12385; PTHR12385; 1.
DR Pfam; PF04515; Choline_transpo; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Phosphoprotein; Reference proteome; Sialic acid;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..705
FT /note="Choline transporter-like protein 2"
FT /id="PRO_0000191716"
FT TOPO_DOM 1..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..231
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..255
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..314
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 336..363
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..384
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 385..455
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 456..478
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 479..503
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 504..524
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 525..562
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 563..583
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 584..598
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 599..619
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 620..637
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 638..658
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 659..705
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 12
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8IWA5"
FT CARBOHYD 186
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 199
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 414
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 705 AA; 79971 MW; 948D253F7DFC0065 CRC64;
MEDQRKYGAY GTPQKYDPTF KGPIYNRGCT DVLCCVLLFL AIVGYVAVGL IAWTHGDPRK
VIYPTDSRGE FCGQKGTKNA NKPFLFYFNI VKCASPLVLL EFQCPTPQIC VEKCPNRYLT
LLNAWNTPEF EYYKQFCVPD FQKNKKGVAQ VLRDGQCPAV LIPSKPLAQR CFPDIHAHKG
VIMVGNATTY EDGHGSRKNI TELVEGAKQA NGILEARQLA MRIFEDYTVS WYWIVIGLVI
AMVLSLLFII LLRFLAGIMV WVMIVLVILV LGYGIFHCYM EYARLRGEAG SDISVLDLGF
QTDFRVYLHL RQTWLAFMII LSILEVIIIL LLIFLRKRIL IAIALIKEAS RAVGYVMCSL
LYPLVTFFLL CLCIAYWAST AVFLSTSNEA VYKILGDSSC PHQGQTCHPE TFFNSTEAHA
CPNARCQFAF YGGESGYHRA LLGLQIFNAF MFFWLANFVL ALGQVTLAGA FASYYWALRK
PDDMPAFPLF AAFGRALRYH TGSLAFGSLI LAIVQIIRVI LEYLDQRLKA AENKFAKFLM
TCLKCCFWCL EKFIKFLNRN AYIMIAIYGT NFCTSARNAF FLLMRNIIRV AVLDKVTDFL
FLLGKLLIVG SVGILAFFFF THRIRIVQDT APPLNYYWVP ILTVIVGSYL IAHGFFSVYG
MCVDTLFLCF LEDLERNNGS SERPYFMSST LKKLLNKTNK KPVES
