CTL4_BOVIN
ID CTL4_BOVIN Reviewed; 707 AA.
AC A3KMY4;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Choline transporter-like protein 4 {ECO:0000250|UniProtKB:Q53GD3};
DE AltName: Full=Solute carrier family 44 member 4 {ECO:0000250|UniProtKB:Q53GD3};
DE AltName: Full=Thiamine pyrophosphate transporter 1 {ECO:0000250|UniProtKB:Q53GD3};
GN Name=SLC44A4 {ECO:0000250|UniProtKB:Q53GD3};
GN Synonyms=CTL4 {ECO:0000250|UniProtKB:Q53GD3},
GN TPPT1 {ECO:0000250|UniProtKB:Q53GD3};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Ascending colon;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Choline transporter that plays a role in the choline-
CC acetylcholine system and is required to the efferent innervation of
CC hair cells in the olivocochlear bundle for the maintenance of
CC physiological function of outer hair cells and the protection of hair
CC cells from acoustic injury (By similarity). Also described as a
CC thiamine pyrophosphate transporter in colon, may mediate the absorption
CC of microbiota-generated thiamine pyrophosphate and contribute to host
CC thiamine (vitamin B1) homeostasis (By similarity).
CC {ECO:0000250|UniProtKB:Q53GD3, ECO:0000250|UniProtKB:Q7T2B0}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q53GD3}; Multi-
CC pass membrane protein {ECO:0000250|UniProtKB:Q53GD3}. Apical cell
CC membrane {ECO:0000250|UniProtKB:Q53GD3}.
CC -!- PTM: N-glycosylated; N-glycosylation of Asn-68 and Asn-391 is required
CC for a proper thiamine pyrophosphate uptake.
CC {ECO:0000250|UniProtKB:Q53GD3}.
CC -!- SIMILARITY: Belongs to the CTL (choline transporter-like) family.
CC {ECO:0000305}.
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DR EMBL; BC133403; AAI33404.1; -; mRNA.
DR RefSeq; NP_001076911.1; NM_001083442.1.
DR AlphaFoldDB; A3KMY4; -.
DR STRING; 9913.ENSBTAP00000042762; -.
DR PaxDb; A3KMY4; -.
DR PRIDE; A3KMY4; -.
DR Ensembl; ENSBTAT00000045367; ENSBTAP00000042762; ENSBTAG00000005675.
DR GeneID; 520625; -.
DR KEGG; bta:520625; -.
DR CTD; 80736; -.
DR VEuPathDB; HostDB:ENSBTAG00000005675; -.
DR VGNC; VGNC:34878; SLC44A4.
DR eggNOG; KOG1362; Eukaryota.
DR GeneTree; ENSGT00940000160576; -.
DR HOGENOM; CLU_017181_3_1_1; -.
DR InParanoid; A3KMY4; -.
DR OMA; GAYCGFG; -.
DR OrthoDB; 329225at2759; -.
DR TreeFam; TF313325; -.
DR Proteomes; UP000009136; Chromosome 23.
DR Bgee; ENSBTAG00000005675; Expressed in olfactory segment of nasal mucosa and 74 other tissues.
DR ExpressionAtlas; A3KMY4; baseline and differential.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015220; F:choline transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0090422; F:thiamine pyrophosphate transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008292; P:acetylcholine biosynthetic process; ISS:UniProtKB.
DR GO; GO:0061526; P:acetylcholine secretion; ISS:UniProtKB.
DR GO; GO:0015871; P:choline transport; ISS:UniProtKB.
DR GO; GO:0035675; P:neuromast hair cell development; ISS:UniProtKB.
DR GO; GO:0032475; P:otolith formation; ISS:UniProtKB.
DR GO; GO:0030307; P:positive regulation of cell growth; ISS:UniProtKB.
DR GO; GO:0030974; P:thiamine pyrophosphate transmembrane transport; ISS:UniProtKB.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR InterPro; IPR007603; Choline_transptr-like.
DR PANTHER; PTHR12385; PTHR12385; 1.
DR Pfam; PF04515; Choline_transpo; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..707
FT /note="Choline transporter-like protein 4"
FT /id="PRO_0000359720"
FT TOPO_DOM 1..33
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..227
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..250
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..307
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 329..356
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 378..452
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..473
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 474..498
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 499..519
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 520..557
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 558..578
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 579..594
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 595..615
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 616..635
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 636..656
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 657..707
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 68
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250|UniProtKB:Q53GD3"
FT CARBOHYD 185
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 196
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250|UniProtKB:Q53GD3"
FT CARBOHYD 296
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 391
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250|UniProtKB:Q53GD3"
FT CARBOHYD 403
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 413
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250|UniProtKB:Q53GD3"
SQ SEQUENCE 707 AA; 78938 MW; FD6FA820133A5A50 CRC64;
MGGKQDQDKE AYGKPAKYDP SFRGPIRNRS CTDIICCVLF FLFILGYIAV GILAWVYGDP
KQVLYPRNST GAYCGIGENK EKPYLLYFNI FSCVLNTNII AIAQNGLKCP TPQVCVSSCP
EASWTVEPRQ FSQTVEQVFY AANRNFCLPG VPGNMQVLQS LQQELCPSFL LPSTPALGRC
FPWTNSTVPE LPGISNTSIS QSISGLLDSL NARDISVKIF EDFAQSWYWI LIALGLALVL
SLLFILLLRL VAGPLVFVLI IGVLGVLAYG IYHCWEEYRV LRDKGASISQ LGFTTNLSAY
RNVQETWLAA LIILAVLEGV LLLMLIFLRQ RICIAIALLK EASRAVGYIM STMFYPLVTF
ALLLVCIAYW AIIALFLATS GQPQYVFWAP NSSLPGCEKV PMNTSCDPME QVNSSCPGLM
CVFQGYQSTG LAQRSLFNLQ IYAVLGLFWT INWVLALGQC VLAGAFASFY WAFHKPRDIP
TFPLGSAFLR TLRYHTGSLA FGALILTLVQ IARVILEYID HKLRGAQNPL TRCILCCFKC
CLWCLEKFIK FLNRNAYIMI AIYGKNFCVS AKNAFMLLMR NIVRVVVLDK VTDLLLFFGK
LLVVGGVGVL SFFFFTGRIP SLGKTFENPQ LNYYWLPIMV SILGAYLIAS GFFSVFGMCV
DTLFLCFLED LERNDGSADR PYYMSKSLLK ILGKKNKGTP GDKKRKK
