CTNA_DICDI
ID CTNA_DICDI Reviewed; 258 AA.
AC Q86IV5; Q556C3; Q9U7C4;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Countin-1;
DE Flags: Precursor;
GN Name=ctnA; ORFNames=DDB_G0274597;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-41, IDENTIFICATION IN
RP THE CF COMPLEX, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, FUNCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=10444594; DOI=10.1101/gad.13.15.1960;
RA Brock D.A., Gomer R.H.;
RT "A cell-counting factor regulating structure size in Dictyostelium.";
RL Genes Dev. 13:1960-1969(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP FUNCTION.
RX PubMed=11090633; DOI=10.1016/s1097-2765(05)00082-1;
RA Roisin-Bouffay C., Jang W., Caprette D.R., Gomer R.H.;
RT "A precise group size in Dictyostelium is generated by a cell-counting
RT factor modulating cell-cell adhesion.";
RL Mol. Cell 6:953-959(2000).
RN [5]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=11737154; DOI=10.1046/j.1440-169x.2001.00615.x;
RA Okuwa T., Katayama T., Takano A., Kodaira K., Yasukawa H.;
RT "Two cell-counting factors regulate the aggregate size of the cellular
RT slime mold Dictyostelium discoideum.";
RL Dev. Growth Differ. 43:735-744(2001).
RN [6]
RP IDENTIFICATION IN THE CF COMPLEX.
RX PubMed=12117815; DOI=10.1242/dev.129.15.3657;
RA Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Ammann R., Gomer R.H.;
RT "The different components of a multisubunit cell number-counting factor
RT have both unique and overlapping functions.";
RL Development 129:3657-3668(2002).
RN [7]
RP IDENTIFICATION IN THE CF COMPLEX.
RX PubMed=12912898; DOI=10.1128/ec.2.4.788-797.2003;
RA Brock D.A., Hatton R.D., Giurgiutiu D.-V., Scott B., Jang W., Ammann R.,
RA Gomer R.H.;
RT "CF45-1, a secreted protein which participates in Dictyostelium group size
RT regulation.";
RL Eukaryot. Cell 2:788-797(2003).
RN [8]
RP IDENTIFICATION IN THE CF COMPLEX.
RX PubMed=16963635; DOI=10.1128/ec.00169-06;
RA Brock D.A., van Egmond W.N., Shamoo Y., Hatton R.D., Gomer R.H.;
RT "A 60-kilodalton protein component of the counting factor complex regulates
RT group size in Dictyostelium discoideum.";
RL Eukaryot. Cell 5:1532-1538(2006).
CC -!- FUNCTION: Cell-counting factor that limits the maximum size of the
CC multicellular structure. May down-regulate the expression of gp24,
CC which mediates cell adhesion. {ECO:0000269|PubMed:10444594,
CC ECO:0000269|PubMed:11090633, ECO:0000269|PubMed:11737154}.
CC -!- SUBUNIT: Component of the counting factor (CF) complex, which includes
CC cf60, cf50, cf45-1 and ctnA. {ECO:0000269|PubMed:10444594,
CC ECO:0000269|PubMed:12117815, ECO:0000269|PubMed:12912898,
CC ECO:0000269|PubMed:16963635}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10444594}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in the vegetative cells and
CC decreases during subsequent developmental stages (6-24 hours).
CC {ECO:0000269|PubMed:10444594, ECO:0000269|PubMed:11737154}.
CC -!- DISRUPTION PHENOTYPE: Cells form a fewer number of larger aggregates.
CC {ECO:0000269|PubMed:10444594}.
CC -!- SIMILARITY: Belongs to the countin family. {ECO:0000305}.
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DR EMBL; AF140780; AAD53482.1; -; Genomic_DNA.
DR EMBL; AAFI02000012; EAL70191.1; -; Genomic_DNA.
DR RefSeq; XP_643887.1; XM_638795.1.
DR AlphaFoldDB; Q86IV5; -.
DR STRING; 44689.DDB0185089; -.
DR TCDB; 1.C.35.4.1; the amoebapore (amoebapore) family.
DR PaxDb; Q86IV5; -.
DR EnsemblProtists; EAL70191; EAL70191; DDB_G0274597.
DR GeneID; 8619313; -.
DR KEGG; ddi:DDB_G0274597; -.
DR dictyBase; DDB_G0274597; ctnA.
DR eggNOG; ENOG502S5J5; Eukaryota.
DR HOGENOM; CLU_095805_0_0_1; -.
DR InParanoid; Q86IV5; -.
DR OMA; CEQWLPG; -.
DR PhylomeDB; Q86IV5; -.
DR PRO; PR:Q86IV5; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005576; C:extracellular region; IDA:dictyBase.
DR GO; GO:0030041; P:actin filament polymerization; IDA:dictyBase.
DR GO; GO:0048870; P:cell motility; IDA:dictyBase.
DR GO; GO:0042593; P:glucose homeostasis; IMP:dictyBase.
DR GO; GO:0098727; P:maintenance of cell number; IDA:dictyBase.
DR GO; GO:0007162; P:negative regulation of cell adhesion; IDA:dictyBase.
DR GO; GO:0010754; P:negative regulation of cGMP-mediated signaling; IMP:dictyBase.
DR GO; GO:0040015; P:negative regulation of multicellular organism growth; IMP:dictyBase.
DR GO; GO:0106071; P:positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway; IMP:dictyBase.
DR GO; GO:0031157; P:regulation of aggregate size involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0032114; P:regulation of glucose-6-phosphatase activity; IMP:dictyBase.
DR GO; GO:0006110; P:regulation of glycolytic process; IMP:dictyBase.
DR GO; GO:0010225; P:response to UV-C; IDA:dictyBase.
DR InterPro; IPR008139; SaposinB_dom.
DR SMART; SM00741; SapB; 1.
DR PROSITE; PS50015; SAP_B; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:10444594"
FT CHAIN 22..258
FT /note="Countin-1"
FT /id="PRO_0000327908"
FT DOMAIN 22..106
FT /note="Saposin B-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT REGION 233..258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 233..251
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 121
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT CARBOHYD 215
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 25..102
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 28..96
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 56..69
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT CONFLICT 45
FT /note="Missing (in Ref. 1; AAD53482)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 258 AA; 26659 MW; 725F37870E78356A CRC64;
MNKLFSLILA LFLVNSAVVS SLDSCSICVD FVGNSLNDLL NIILNSGVIG TCGDLCSAVP
GGQIVDTVCD LLCDYVGVDE FIKLISDVDP DPIYICEKIS VCKTNDNAAA SLDLVQINPQ
NGTVGGTFTL SIAYTVTNTI ATGQLAFNII DPTGNAFGDA VLLVQQSPEQ YTQQFQFQAT
PSEQESFPNG LYTVQALVCE GSCGSPHPNT YTLANGTTTF TISGADSSSM SGAGSFSGSS
QSTQTGAASG SGSGFALF
