CTNB_DICDI
ID CTNB_DICDI Reviewed; 254 AA.
AC Q8WSR7; Q54W80;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Countin-2;
DE Flags: Precursor;
GN Name=ctnB; ORFNames=DDB_G0279797;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=11737154; DOI=10.1046/j.1440-169x.2001.00615.x;
RA Okuwa T., Katayama T., Takano A., Kodaira K., Yasukawa H.;
RT "Two cell-counting factors regulate the aggregate size of the cellular
RT slime mold Dictyostelium discoideum.";
RL Dev. Growth Differ. 43:735-744(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Cell-counting factor that limits the minimum size of the
CC multicellular structure. May up-regulate the expression of both gp24
CC and gp80, which mediate cell adhesion. {ECO:0000269|PubMed:11737154}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11737154}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the vegetative cells. Increases
CC during aggregation to mound stages (6-12 hours) and decreases at late
CC developmental stages (18-24 hours). {ECO:0000269|PubMed:11737154}.
CC -!- DISRUPTION PHENOTYPE: Cells form a higher number of smaller aggregates.
CC {ECO:0000269|PubMed:11737154}.
CC -!- SIMILARITY: Belongs to the countin family. {ECO:0000305}.
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DR EMBL; AB067573; BAB84187.1; -; mRNA.
DR EMBL; AAFI02000033; EAL67512.1; -; Genomic_DNA.
DR RefSeq; XP_641512.1; XM_636420.1.
DR AlphaFoldDB; Q8WSR7; -.
DR STRING; 44689.DDB0214993; -.
DR PaxDb; Q8WSR7; -.
DR EnsemblProtists; EAL67512; EAL67512; DDB_G0279797.
DR GeneID; 8622253; -.
DR KEGG; ddi:DDB_G0279797; -.
DR dictyBase; DDB_G0279797; ctnB.
DR eggNOG; ENOG502S5J5; Eukaryota.
DR HOGENOM; CLU_095805_0_0_1; -.
DR InParanoid; Q8WSR7; -.
DR OMA; NGQCMSH; -.
DR PhylomeDB; Q8WSR7; -.
DR PRO; PR:Q8WSR7; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0098609; P:cell-cell adhesion; IMP:dictyBase.
DR InterPro; IPR008139; SaposinB_dom.
DR SMART; SM00741; SapB; 1.
DR PROSITE; PS50015; SAP_B; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..254
FT /note="Countin-2"
FT /id="PRO_0000327909"
FT DOMAIN 22..107
FT /note="Saposin B-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT REGION 231..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..248
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 110
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT CARBOHYD 219
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 26..103
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 29..97
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 56..68
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
SQ SEQUENCE 254 AA; 26347 MW; 359482BE25C6D3AF CRC64;
MMIKYITIAI LFIASLVKAD LQFSLCPTCV DFINNDMGDL EKIISGGIAT SCGAVCSLLP
NNIEQGACNL LCDIVGIDEF LKVFNNIGED ADPVWICEEL TVCPKNQNSN ATVLTSDVSP
ASGPHGTTFT IGVAYKVEST LGTGEVAVMV TDPTGSNGFG DAQLIVNTQP GQYSTSFSFA
ATPSEDEQFP AGVYQVQLMI CEGSCGAKHS VTFNSVYANF TVTSGPSVTG QMTGTGSGSG
SGSGSSSGAA YLRY
