CTNS_ARATH
ID CTNS_ARATH Reviewed; 270 AA.
AC P57758;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Cystinosin homolog;
GN OrderedLocusNames=At5g40670; ORFNames=MNF13.23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Thought to transport cystine out of lysosomes. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cystinosin (TC 2.A.43.1) family.
CC {ECO:0000305}.
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DR EMBL; AB009052; BAB08539.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94580.1; -; Genomic_DNA.
DR EMBL; AY052257; AAK97727.1; -; mRNA.
DR EMBL; AY060522; AAL31135.1; -; mRNA.
DR RefSeq; NP_198883.1; NM_123432.4.
DR AlphaFoldDB; P57758; -.
DR BioGRID; 19318; 6.
DR IntAct; P57758; 6.
DR STRING; 3702.AT5G40670.1; -.
DR TCDB; 2.A.43.1.4; the lysosomal cystine transporter (lct) family.
DR PaxDb; P57758; -.
DR PRIDE; P57758; -.
DR ProteomicsDB; 220317; -.
DR EnsemblPlants; AT5G40670.1; AT5G40670.1; AT5G40670.
DR GeneID; 834067; -.
DR Gramene; AT5G40670.1; AT5G40670.1; AT5G40670.
DR KEGG; ath:AT5G40670; -.
DR Araport; AT5G40670; -.
DR TAIR; locus:2168843; AT5G40670.
DR eggNOG; KOG3145; Eukaryota.
DR HOGENOM; CLU_046327_0_0_1; -.
DR InParanoid; P57758; -.
DR OMA; WIDVIYT; -.
DR OrthoDB; 1138417at2759; -.
DR PhylomeDB; P57758; -.
DR PRO; PR:P57758; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P57758; baseline and differential.
DR Genevisible; P57758; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR GO; GO:0015184; F:L-cystine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015811; P:L-cystine transport; IBA:GO_Central.
DR InterPro; IPR005282; LC_transporter.
DR InterPro; IPR006603; PQ-loop_rpt.
DR PANTHER; PTHR13131; PTHR13131; 1.
DR Pfam; PF04193; PQ-loop; 2.
DR SMART; SM00679; CTNS; 2.
DR TIGRFAMs; TIGR00951; 2A43; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Lysosome; Membrane; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..270
FT /note="Cystinosin homolog"
FT /id="PRO_0000205518"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 9..75
FT /note="PQ-loop 1"
FT DOMAIN 151..213
FT /note="PQ-loop 2"
FT REGION 250..270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 52
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 174
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 270 AA; 31027 MW; DB54C04B882DA4F0 CRC64;
MASWNSIPLE ISYEIVGWIA FASWSISFYP QLILNFRRRS VVGLNFDFVM LNLTKHSSYM
IYNVCLYFSP VIQKQYFDTY GDKEMIPVAA NDVAFSIHAV VMTAVTLFQI FIYERGPQKV
SRLAIGIVVV VWGFAAICFF IALPTHSWLW LISIFNSIQV FMTCVKYIPQ AKMNFTRKST
VGWSIGNILL DFTGGLANYL QMVIQSIDQN SWKNFYGNMG KTLLSLISIF FDILFMFQHY
VLYPEKKVSK SPETGEESNE PLIDSSHEHV
