ID   CTNS_DICDI              Reviewed;         284 AA.
AC   Q54WT7;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Cystinosin homolog;
GN   Name=ctns; ORFNames=DDB_G0279445;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Cystine/H(+) symporter that mediates export of cystine, the
CC       oxidized dimer of cysteine, from lysosomes.
CC       {ECO:0000250|UniProtKB:O60931}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+)(out) + L-cystine(out) = H(+)(in) + L-cystine(in);
CC         Xref=Rhea:RHEA:66172, ChEBI:CHEBI:15378, ChEBI:CHEBI:35491;
CC         Evidence={ECO:0000250|UniProtKB:O60931};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:66173;
CC         Evidence={ECO:0000250|UniProtKB:O60931};
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:O60931};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the cystinosin family. {ECO:0000305}.
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DR   EMBL; AAFI02000031; EAL67661.1; -; Genomic_DNA.
DR   RefSeq; XP_641630.1; XM_636538.1.
DR   AlphaFoldDB; Q54WT7; -.
DR   STRING; 44689.DDB0237502; -.
DR   PaxDb; Q54WT7; -.
DR   EnsemblProtists; EAL67661; EAL67661; DDB_G0279445.
DR   GeneID; 8622036; -.
DR   KEGG; ddi:DDB_G0279445; -.
DR   dictyBase; DDB_G0279445; ctns.
DR   eggNOG; KOG3145; Eukaryota.
DR   HOGENOM; CLU_046327_0_0_1; -.
DR   InParanoid; Q54WT7; -.
DR   OMA; WIDVIYT; -.
DR   PhylomeDB; Q54WT7; -.
DR   Reactome; R-DDI-425393; Transport of inorganic cations/anions and amino acids/oligopeptides.
DR   Reactome; R-DDI-5223345; Miscellaneous transport and binding events.
DR   PRO; PR:Q54WT7; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR   GO; GO:0015184; F:L-cystine transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0015811; P:L-cystine transport; IBA:GO_Central.
DR   InterPro; IPR005282; LC_transporter.
DR   InterPro; IPR006603; PQ-loop_rpt.
DR   PANTHER; PTHR13131; PTHR13131; 1.
DR   Pfam; PF04193; PQ-loop; 2.
DR   SMART; SM00679; CTNS; 2.
DR   TIGRFAMs; TIGR00951; 2A43; 1.
PE   3: Inferred from homology;
KW   Lysosome; Membrane; Reference proteome; Repeat; Symport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..284
FT                   /note="Cystinosin homolog"
FT                   /id="PRO_0000328325"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        216..236
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          4..70
FT                   /note="PQ-loop 1"
FT   DOMAIN          154..208
FT                   /note="PQ-loop 2"
FT   REGION          247..269
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   284 AA;  32514 MW;  2B25E572849086E0 CRC64;
     MSALSIISII IGWIYFACWS LSFYPQVILN FRKKNVIGLS FDFLLFNITG YACYSVFNSV
     LYFDKLVKNE YYDKYGPPIP VQQSDIAFAI HGFVLTAITI IQCFIYDRGN QKNSKLGIGI
     ATLIWVSLIV MTILGFSNVF TWLWVINYYS YVKLFITFIK YIPQAYLNFK NKSTSGWSVH
     NVLLDFSGGV LSLLQMFLDV ADSGNWNIFT GDPVKLGLSL FSIAFDILFI IQHYILYRNP
     KSKGYQNLND NNIPNNNNNN NNNINNNTPH QIIINNSLIG EEDQ