CTNS_DROME
ID CTNS_DROME Reviewed; 397 AA.
AC Q9VCR7; Q8MS51;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Cystinosin homolog;
GN ORFNames=CG17119;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-86, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=Oregon-R; TISSUE=Head;
RX PubMed=17893096; DOI=10.1093/glycob/cwm097;
RA Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L.,
RA Panin V.;
RT "Identification of N-glycosylated proteins from the central nervous system
RT of Drosophila melanogaster.";
RL Glycobiology 17:1388-1403(2007).
CC -!- FUNCTION: Cystine/H(+) symporter that mediates export of cystine, the
CC oxidized dimer of cysteine, from lysosomes.
CC {ECO:0000250|UniProtKB:O60931}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + L-cystine(out) = H(+)(in) + L-cystine(in);
CC Xref=Rhea:RHEA:66172, ChEBI:CHEBI:15378, ChEBI:CHEBI:35491;
CC Evidence={ECO:0000250|UniProtKB:O60931};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:66173;
CC Evidence={ECO:0000250|UniProtKB:O60931};
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:O60931};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the cystinosin family. {ECO:0000305}.
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DR EMBL; AE014297; AAF56088.2; -; Genomic_DNA.
DR EMBL; AY119096; AAM50956.1; -; mRNA.
DR RefSeq; NP_651116.1; NM_142859.3.
DR RefSeq; NP_788720.2; NM_176543.2.
DR AlphaFoldDB; Q9VCR7; -.
DR STRING; 7227.FBpp0083750; -.
DR TCDB; 2.A.43.1.2; the lysosomal cystine transporter (lct) family.
DR GlyGen; Q9VCR7; 2 sites.
DR iPTMnet; Q9VCR7; -.
DR PaxDb; Q9VCR7; -.
DR PRIDE; Q9VCR7; -.
DR DNASU; 42723; -.
DR EnsemblMetazoa; FBtr0084357; FBpp0083750; FBgn0039045.
DR EnsemblMetazoa; FBtr0344299; FBpp0310702; FBgn0039045.
DR GeneID; 42723; -.
DR KEGG; dme:Dmel_CG17119; -.
DR UCSC; CG17119-RA; d. melanogaster.
DR FlyBase; FBgn0039045; CG17119.
DR VEuPathDB; VectorBase:FBgn0039045; -.
DR eggNOG; KOG3145; Eukaryota.
DR GeneTree; ENSGT00390000005338; -.
DR HOGENOM; CLU_046327_1_1_1; -.
DR InParanoid; Q9VCR7; -.
DR OMA; WIDVIYT; -.
DR OrthoDB; 1138417at2759; -.
DR PhylomeDB; Q9VCR7; -.
DR Reactome; R-DME-425393; Transport of inorganic cations/anions and amino acids/oligopeptides.
DR Reactome; R-DME-5223345; Miscellaneous transport and binding events.
DR BioGRID-ORCS; 42723; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 42723; -.
DR PRO; PR:Q9VCR7; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039045; Expressed in adult Malpighian tubule (Drosophila) and 24 other tissues.
DR ExpressionAtlas; Q9VCR7; differential.
DR Genevisible; Q9VCR7; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005765; C:lysosomal membrane; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; ISS:FlyBase.
DR GO; GO:0015184; F:L-cystine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0003333; P:amino acid transmembrane transport; ISS:FlyBase.
DR GO; GO:0015811; P:L-cystine transport; IBA:GO_Central.
DR InterPro; IPR005282; LC_transporter.
DR InterPro; IPR006603; PQ-loop_rpt.
DR InterPro; IPR010916; TonB_box_CS.
DR PANTHER; PTHR13131; PTHR13131; 1.
DR Pfam; PF04193; PQ-loop; 2.
DR SMART; SM00679; CTNS; 2.
DR TIGRFAMs; TIGR00951; 2A43; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Lysosome; Membrane; Reference proteome; Repeat; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..397
FT /note="Cystinosin homolog"
FT /id="PRO_0000205517"
FT TOPO_DOM 1..126
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..166
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..210
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 232..242
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..266
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..302
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 324..340
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..397
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 128..194
FT /note="PQ-loop 1"
FT DOMAIN 268..333
FT /note="PQ-loop 2"
FT REGION 373..397
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 43
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 86
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17893096"
SQ SEQUENCE 397 AA; 44588 MW; D89CE9B93F4BE645 CRC64;
MDFSTHRLTT LLLLLLATVA LGNAQSSQLT VDSHDITVLL NSNETFLVFA NGLLDSDVEV
ALGTDSEDHL LLDPATFVYP AGSTRNQSVV ITGLKAGNVK VVADSDDANK EIVKDVFVRV
TVAKSRALIY TSIIFGWVYF VAWSVSFYPQ IWSNYRRKSV EGLNFDFLAL NIVGFTLYSM
FNCGLYFIED LQNEYEVRYP LGVNPVMLND VVFSLHAMFA TCITILQCFF YQRAQQRVSF
IAYGILAIFA VVVVVSAGLA GGSVIHWLDF LYYCSYVKLT ITIIKYVPQA LMNYRRKSTS
GWSIGNILLD FTGGTLSMLQ MILNAHNYDD WVSIFGDPTK FGLGLFSVLF DVFFMLQHYV
FYRHSRESSS SDLTTVTDVQ NRTNESPPPS EVTTEKY
