CTPE_MYCS2
ID CTPE_MYCS2 Reviewed; 791 AA.
AC A0R3Y2; I7GEK0;
DT 25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Calcium-transporting ATPase CtpE {ECO:0000305};
DE EC=7.2.2.10 {ECO:0000269|PubMed:28951477};
DE AltName: Full=Calcium uptake transporter {ECO:0000303|PubMed:28951477};
GN Name=ctpE {ECO:0000312|EMBL:AFP41926.1};
GN OrderedLocusNames=MSMEG_5636 {ECO:0000312|EMBL:ABK72389.1},
GN MSMEI_5486 {ECO:0000312|EMBL:AFP41926.1};
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=28951477; DOI=10.1128/mbio.01388-17;
RA Gupta H.K., Shrivastava S., Sharma R.;
RT "A novel calcium uptake transporter of uncharacterized P-type ATPase family
RT supplies calcium for cell surface integrity in Mycobacterium smegmatis.";
RL MBio 8:E01388-E01388(2017).
CC -!- FUNCTION: P-type ATPase involved in specific uptake of calcium.
CC Essential for growth and maintenance of cell surface integrity under
CC Ca(2+)-deficient conditions. {ECO:0000269|PubMed:28951477}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + Ca(2+)(in) + H2O = ADP + Ca(2+)(out) + H(+) + phosphate;
CC Xref=Rhea:RHEA:18105, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29108, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=7.2.2.10;
CC Evidence={ECO:0000269|PubMed:28951477};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Expression is negatively regulated by Ca(2+).
CC {ECO:0000269|PubMed:28951477}.
CC -!- DISRUPTION PHENOTYPE: Disruption of the gene leads to impaired growth
CC under Ca(2+)-deficient conditions. Mutant also shows hypersensitivity
CC to polymyxin B, increased biofilm formation and higher cell
CC aggregation, indicating cell envelope defects.
CC {ECO:0000269|PubMed:28951477}.
CC -!- SIMILARITY: Belongs to the cation transport ATPase (P-type) (TC 3.A.3)
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000480; ABK72389.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP41926.1; -; Genomic_DNA.
DR RefSeq; WP_011730674.1; NZ_SIJM01000034.1.
DR RefSeq; YP_889870.1; NC_008596.1.
DR AlphaFoldDB; A0R3Y2; -.
DR SMR; A0R3Y2; -.
DR STRING; 246196.MSMEI_5486; -.
DR PRIDE; A0R3Y2; -.
DR EnsemblBacteria; ABK72389; ABK72389; MSMEG_5636.
DR EnsemblBacteria; AFP41926; AFP41926; MSMEI_5486.
DR GeneID; 66736932; -.
DR KEGG; msg:MSMEI_5486; -.
DR KEGG; msm:MSMEG_5636; -.
DR PATRIC; fig|246196.19.peg.5492; -.
DR eggNOG; COG0474; Bacteria.
DR OMA; RLGRKQC; -.
DR OrthoDB; 178322at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005388; F:P-type calcium transporter activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.1110.10; -; 1.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR023299; ATPase_P-typ_cyto_dom_N.
DR InterPro; IPR018303; ATPase_P-typ_P_site.
DR InterPro; IPR023298; ATPase_P-typ_TM_dom_sf.
DR InterPro; IPR008250; ATPase_P-typ_transduc_dom_A_sf.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR001757; P_typ_ATPase.
DR InterPro; IPR044492; P_typ_ATPase_HD_dom.
DR SFLD; SFLDF00027; p-type_atpase; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR SUPFAM; SSF81653; SSF81653; 1.
DR SUPFAM; SSF81665; SSF81665; 1.
DR TIGRFAMs; TIGR01494; ATPase_P-type; 2.
DR PROSITE; PS00154; ATPASE_E1_E2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Calcium; Calcium transport; Cell membrane; Ion transport;
KW Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Translocase; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..791
FT /note="Calcium-transporting ATPase CtpE"
FT /id="PRO_0000443813"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 596..616
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 627..647
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 664..684
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 697..717
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 725..745
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 757..777
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 299
FT /note="4-aspartylphosphate intermediate"
FT /evidence="ECO:0000250|UniProtKB:Q5ZWR1"
FT BINDING 299
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q5ZWR1"
FT BINDING 301
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q5ZWR1"
FT BINDING 530
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q5ZWR1"
SQ SEQUENCE 791 AA; 83867 MW; C0B3F8F89692954E CRC64;
MTTMAAAGLT DAEVAQRIAE GKTNDVPTRA ARTVSEIVRA NVFTRINAIL GVLFVIVLST
GSVINGAFGL LIIANSAIGI IQEIRAKQTL DKLAIVGQAK PTVRRQSGTR AVLPSEVVLD
DIIELGPGDQ IVVDGEVVEE TNLEVDESLL TGEADPIAKD AGDPVMSGSF VVAGSGAYRA
TKVGREAYAA KLAEEASKFT LVKSELRNGI NKILQFITYL LVPAGLLTIY TQLFTTDAGW
REAVLRMVGA LVPMVPEGLV LMTSIAFAVG VVRLGRRQCL VNELPAIEGL ARVDVVCADK
TGTLTENGMR VSDLKSLTEG HVADVLAQLA SDDARPNASM AAIAEAYQTP PGWSATATAP
FKSATKWSGT SYGEHGNWVI GAPDVLLDPA SPVAEEAERI GAQGLRVLLL GSSDRSVDAP
DAPGVVTPAA LVVLEQRIRP DAGDTLDYFA SQHVSVKVIS GDNAVSVGAV AGKLGLHGET
MDARRLPEQP EKLAETLEEC TTFGRVRPDQ KRAMVHALQS RGHTVAMTGD GVNDVLALKD
SDIGVAMGSG SSASRAVAQI VLLDNKFATL PYVVGEGRRV IGNIERVSNL FLTKTVYSVL
LAILVGIGGL SAKIFGTDPL LFPFQPIHVT IAAWFTIGIP AFILSLAPNN ERAKTGFVRR
VMTAALPSGL VVGTATFVSY LVAYQGREAT PVEQTQASTA ALITLLASSL WVLAVVARPY
QWWRVLLVAC SMLAYVLIFS IPLAQELFML DPTNMKVTSV ALGIGLAGAA LIEVLWWVQG
RVLGEERRVW R
