CTPH_PSEAE
ID CTPH_PSEAE Reviewed; 568 AA.
AC G3XDA3; Q7DCC4; Q9KWF2;
DT 22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 1.
DT 03-AUG-2022, entry version 50.
DE RecName: Full=Methyl-accepting chemotaxis protein CtpH {ECO:0000305};
GN Name=ctpH {ECO:0000303|PubMed:10852870}; OrderedLocusNames=PA2561;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A CHEMORECEPTOR, AND
RP INDUCTION.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10852870; DOI=10.1128/jb.182.12.3400-3404.2000;
RA Wu H., Kato J., Kuroda A., Ikeda T., Takiguchi N., Ohtake H.;
RT "Identification and characterization of two chemotactic transducers for
RT inorganic phosphate in Pseudomonas aeruginosa.";
RL J. Bacteriol. 182:3400-3404(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND DOMAIN.
RX PubMed=27353565; DOI=10.1038/srep28967;
RA Rico-Jimenez M., Reyes-Darias J.A., Ortega A., Diez Pena A.I., Morel B.,
RA Krell T.;
RT "Two different mechanisms mediate chemotaxis to inorganic phosphate in
RT Pseudomonas aeruginosa.";
RL Sci. Rep. 6:28967-28967(2016).
CC -!- FUNCTION: Chemotactic-signal transducers respond to changes in the
CC concentration of attractants and repellents in the environment,
CC transduce a signal from the outside to the inside of the cell, and
CC facilitate sensory adaptation through the variation of the level of
CC methylation. Chemoreceptor for inorganic phosphate, which is required
CC for taxis at high concentrations of phosphate (PubMed:10852870).
CC Recognizes inorganic phosphate directly. Can also bind to other
CC components that have a pyrophosphate group, including ATP and ADP
CC (PubMed:27353565). {ECO:0000269|PubMed:10852870,
CC ECO:0000269|PubMed:27353565}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:27353565}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Constitutively expressed. {ECO:0000269|PubMed:10852870}.
CC -!- DOMAIN: Binding to inorganic phosphate induces dimerization and
CC stabilization of the ligand binding domain.
CC {ECO:0000269|PubMed:27353565}.
CC -!- SIMILARITY: Belongs to the methyl-accepting chemotaxis (MCP) protein
CC family. {ECO:0000305}.
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DR EMBL; AB039332; BAA96488.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG05949.1; -; Genomic_DNA.
DR PIR; E83325; E83325.
DR RefSeq; NP_251251.1; NC_002516.2.
DR RefSeq; WP_010895626.1; NZ_QZGE01000008.1.
DR AlphaFoldDB; G3XDA3; -.
DR SMR; G3XDA3; -.
DR STRING; 287.DR97_5607; -.
DR PaxDb; G3XDA3; -.
DR EnsemblBacteria; AAG05949; AAG05949; PA2561.
DR GeneID; 882739; -.
DR KEGG; pae:PA2561; -.
DR PATRIC; fig|208964.12.peg.2681; -.
DR PseudoCAP; PA2561; -.
DR HOGENOM; CLU_000445_107_27_6; -.
DR InParanoid; G3XDA3; -.
DR OMA; IALVCFQ; -.
DR PhylomeDB; G3XDA3; -.
DR BioCyc; PAER208964:G1FZ6-2597-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:0006935; P:chemotaxis; IMP:PseudoCAP.
DR GO; GO:0010247; P:detection of phosphate ion; IMP:PseudoCAP.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004090; Chemotax_Me-accpt_rcpt.
DR InterPro; IPR003660; HAMP_dom.
DR InterPro; IPR004089; MCPsignal_dom.
DR Pfam; PF00672; HAMP; 1.
DR Pfam; PF00015; MCPsignal; 1.
DR PRINTS; PR00260; CHEMTRNSDUCR.
DR SMART; SM00304; HAMP; 2.
DR SMART; SM00283; MA; 1.
DR PROSITE; PS50111; CHEMOTAXIS_TRANSDUC_2; 1.
DR PROSITE; PS50885; HAMP; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Chemotaxis; Membrane; Methylation;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..568
FT /note="Methyl-accepting chemotaxis protein CtpH"
FT /id="PRO_0000424878"
FT TOPO_DOM 1..39
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:27353565"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 61..216
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:27353565"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 238..568
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:27353565"
FT DOMAIN 239..291
FT /note="HAMP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT DOMAIN 296..532
FT /note="Methyl-accepting transducer"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00284"
SQ SEQUENCE 568 AA; 61573 MW; E7B941123C6E5401 CRC64;
MPASPGHRDV LGCLVAACVP VQPGNPSRRS MLQQSLRAQI LVLLGGSLAA LLLIALACFG
SLTGDVRAYR ELLGGPVRAA QLIDEANLQF RGQVQEWKNV LLRGRQTEAQ TKYWSQFEAQ
ERAVQDILGR LGSVAEGELK DRVERLREEH RRLGTAYRQG RQRFLEAGAD PIAGDQAVTG
IDRATTAQMQ TLRDELHQAS DLHSSSISAE ARRTMLLGSL VLIGASLAVA LLSLWLVNRN
LVRPVQRLIE HIAQLSHGDF GERIEIRRKD ELGKLALAAN TLRDFLVDIF DRLRRSTRDL
DSASGSLNAI ASLMAAGTRE QFSRTDQVAT AMQEMSATAQ EVARYAGDAA RAADEADDSA
QRGEDVMEET IRSIGEMRKE IDHTVEVIRQ LESDSGRIGK VLDVIRGIAE QTNLLALNAA
IEAARAGDAG RGFAVVADEV RTLAQRTAES IAEIHQIIDT VQNGAVNAAR AIESGQSRSE
AGAEQVANAG AMLRQITASV ESIRDMNRQI ATAAEEQTAV AEEISRNLTE IASIASSNQE
QVEQTEAASR DLHGLSAQLG DALQRLRA
