CTPL_PSEAE
ID CTPL_PSEAE Reviewed; 657 AA.
AC Q9HUW6; Q9KWF1;
DT 22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Methyl-accepting chemotaxis protein CtpL {ECO:0000305};
GN Name=ctpL {ECO:0000303|PubMed:10852870}; OrderedLocusNames=PA4844;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A CHEMORECEPTOR, AND
RP INDUCTION.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10852870; DOI=10.1128/jb.182.12.3400-3404.2000;
RA Wu H., Kato J., Kuroda A., Ikeda T., Takiguchi N., Ohtake H.;
RT "Identification and characterization of two chemotactic transducers for
RT inorganic phosphate in Pseudomonas aeruginosa.";
RL J. Bacteriol. 182:3400-3404(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP FUNCTION.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=24038698; DOI=10.1128/aem.02428-13;
RA Vangnai A.S., Takeuchi K., Oku S., Kataoka N., Nitisakulkan T., Tajima T.,
RA Kato J.;
RT "Identification of CtpL as a chromosomally encoded chemoreceptor for 4-
RT chloroaniline and catechol in Pseudomonas aeruginosa PAO1.";
RL Appl. Environ. Microbiol. 79:7241-7248(2013).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=27353565; DOI=10.1038/srep28967;
RA Rico-Jimenez M., Reyes-Darias J.A., Ortega A., Diez Pena A.I., Morel B.,
RA Krell T.;
RT "Two different mechanisms mediate chemotaxis to inorganic phosphate in
RT Pseudomonas aeruginosa.";
RL Sci. Rep. 6:28967-28967(2016).
CC -!- FUNCTION: Chemotactic-signal transducers respond to changes in the
CC concentration of attractants and repellents in the environment,
CC transduce a signal from the outside to the inside of the cell, and
CC facilitate sensory adaptation through the variation of the level of
CC methylation. Chemoreceptor for inorganic phosphate, which is required
CC for taxis at low concentrations of phosphate. Is also responsible for
CC the positive chemotaxis toward 4-chloroaniline (4CA) and catechol
CC (PubMed:10852870, PubMed:24038698). Does not recognize inorganic
CC phosphate directly, but via a complex between the periplasmic protein
CC PstS and inorganic phosphate (PubMed:27353565).
CC {ECO:0000269|PubMed:10852870, ECO:0000269|PubMed:24038698,
CC ECO:0000269|PubMed:27353565}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:27353565}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Induced by phosphate limitation, via the PhoR/PhoB two-
CC component regulatory system. Repressed by PhoU under conditions of
CC phosphate excess. {ECO:0000269|PubMed:10852870}.
CC -!- SIMILARITY: Belongs to the methyl-accepting chemotaxis (MCP) protein
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA96489.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB039333; BAA96489.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AE004091; AAG08229.1; -; Genomic_DNA.
DR PIR; E83041; E83041.
DR RefSeq; NP_253531.1; NC_002516.2.
DR RefSeq; WP_003112328.1; NZ_QZGE01000002.1.
DR AlphaFoldDB; Q9HUW6; -.
DR SMR; Q9HUW6; -.
DR STRING; 287.DR97_2195; -.
DR PaxDb; Q9HUW6; -.
DR PRIDE; Q9HUW6; -.
DR EnsemblBacteria; AAG08229; AAG08229; PA4844.
DR GeneID; 879569; -.
DR KEGG; pae:PA4844; -.
DR PATRIC; fig|208964.12.peg.5076; -.
DR PseudoCAP; PA4844; -.
DR HOGENOM; CLU_000445_107_27_6; -.
DR InParanoid; Q9HUW6; -.
DR OMA; LWWSQRA; -.
DR PhylomeDB; Q9HUW6; -.
DR BioCyc; PAER208964:G1FZ6-4958-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:0006935; P:chemotaxis; IMP:PseudoCAP.
DR GO; GO:0010247; P:detection of phosphate ion; IMP:PseudoCAP.
DR GO; GO:0050918; P:positive chemotaxis; IMP:PseudoCAP.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004090; Chemotax_Me-accpt_rcpt.
DR InterPro; IPR003660; HAMP_dom.
DR InterPro; IPR004089; MCPsignal_dom.
DR Pfam; PF00015; MCPsignal; 1.
DR PRINTS; PR00260; CHEMTRNSDUCR.
DR SMART; SM00283; MA; 1.
DR PROSITE; PS50111; CHEMOTAXIS_TRANSDUC_2; 1.
DR PROSITE; PS50885; HAMP; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Chemotaxis; Membrane; Methylation;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..657
FT /note="Methyl-accepting chemotaxis protein CtpL"
FT /id="PRO_0000424879"
FT TOPO_DOM 1..5
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:27353565"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 27..305
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:27353565"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..657
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:27353565"
FT DOMAIN 327..380
FT /note="HAMP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT DOMAIN 385..621
FT /note="Methyl-accepting transducer"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00284"
SQ SEQUENCE 657 AA; 70783 MW; 8CA115973D07CC69 CRC64;
MRLKQLTNLN TLLLLTVCLA LGITLWWSQR AMERPFQLLD QYLELSQRFD EQVARNIRQY
LGSGDAVRQQ AALQALESLA EALPELPPDL ARTLAPSLAE LREFSAGDLL AAGKLAGDPQ
GLLLQAERDL TGNLEQWSAY LDAAAGQPQA GAYRTPLLLA SLHLTRLSLA RAKLVESANP
ALAGDVEREL ANLREQAGRI EALPLLGVLD EQRSASDDFA AMMGLAGDAE AGAGNAEDRG
VALRRELASL LQRYPDELRR TRDLIERRQQ LSADTGARLD AVRQALATLE PQVRGERQRL
QGQVRLIQGG MIALILLIAL AIDSLQRRLA RVLGQLVPAL SAWADGDFSR PISLRTRTED
LRNLEDSLNR LRSFLAELVG AIHRRAEQVA GSSQTLAEVS SGLHAGVERQ AGDTGQIRDA
LGDMEAAIQQ VAGDASQTAD ASRSAGQAVE HGQRVIGESL GGLRELVDEV QGNAQSIERL
AEESATIGSV LTVIRSIAEQ TNLLALNAAI EAARAGDQGR GFAVVAEEVR SLAQRTAGAT
EEIQQLIGRL QQAARQSVEA MRSQVEHAER TAEQAGAAEG ALDEVVAAIH TIGVMAERIA
EGSTQQSQAV GEIRSHSERI HALGGENLRL IGHSREQGEQ LRQLGGDLRT TVQAFRL
