CTR1_YEAST
ID CTR1_YEAST Reviewed; 406 AA.
AC P49573; D6W4C2;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Copper transport protein CTR1;
DE Short=Copper transporter 1;
GN Name=CTR1; OrderedLocusNames=YPR124W; ORFNames=P9642.3;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=8293472; DOI=10.1016/0092-8674(94)90345-x;
RA Dancis A., Yuan D.S., Haile D., Askwith C., Eide D., Moehle C., Kaplan J.,
RA Klausner R.D.;
RT "Molecular characterization of a copper transport protein in S. cerevisiae:
RT an unexpected role for copper in iron transport.";
RL Cell 76:393-402(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP CHARACTERIZATION.
RX PubMed=7929270; DOI=10.1016/s0021-9258(18)47300-0;
RA Dancis A., Haile D., Yuan D.S., Klausner R.D.;
RT "The Saccharomyces cerevisiae copper transport protein (Ctr1p). Biochemical
RT characterization, regulation by copper, and physiologic role in copper
RT uptake.";
RL J. Biol. Chem. 269:25660-25667(1994).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-344; THR-356 AND SER-369, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-344 AND SER-349, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-345, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- FUNCTION: Required for high affinity copper (probably reduced Cu I)
CC transport into the cell.
CC -!- SUBUNIT: Oligomer.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- INDUCTION: By copper deprivation, and repressed by copper sufficiency.
CC -!- PTM: Extensively O-glycosylated.
CC -!- MISCELLANEOUS: Present with 1180 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U02511; AAA17369.1; -; Unassigned_DNA.
DR EMBL; U40828; AAB68064.1; -; Genomic_DNA.
DR EMBL; AY693062; AAT93081.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11538.1; -; Genomic_DNA.
DR PIR; A53010; A53010.
DR RefSeq; NP_015449.1; NM_001184221.1.
DR AlphaFoldDB; P49573; -.
DR BioGRID; 36292; 99.
DR DIP; DIP-6447N; -.
DR IntAct; P49573; 6.
DR MINT; P49573; -.
DR STRING; 4932.YPR124W; -.
DR TCDB; 1.A.56.2.1; the copper transporter (ctr) family.
DR iPTMnet; P49573; -.
DR MaxQB; P49573; -.
DR PaxDb; P49573; -.
DR PRIDE; P49573; -.
DR EnsemblFungi; YPR124W_mRNA; YPR124W; YPR124W.
DR GeneID; 856241; -.
DR KEGG; sce:YPR124W; -.
DR SGD; S000006328; CTR1.
DR VEuPathDB; FungiDB:YPR124W; -.
DR eggNOG; ENOG502QVCV; Eukaryota.
DR HOGENOM; CLU_819370_0_0_1; -.
DR InParanoid; P49573; -.
DR OMA; WCLEVHW; -.
DR BioCyc; YEAST:G3O-34262-MON; -.
DR PRO; PR:P49573; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; P49573; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IDA:CACAO.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0005375; F:copper ion transmembrane transporter activity; IMP:SGD.
DR GO; GO:0006878; P:cellular copper ion homeostasis; IBA:GO_Central.
DR GO; GO:0015677; P:copper ion import; IMP:SGD.
DR InterPro; IPR007274; Cop_transporter.
DR PANTHER; PTHR12483; PTHR12483; 2.
DR Pfam; PF04145; Ctr; 1.
PE 1: Evidence at protein level;
KW Copper; Copper transport; Glycoprotein; Ion transport; Isopeptide bond;
KW Membrane; Phosphoprotein; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport; Ubl conjugation.
FT CHAIN 1..406
FT /note="Copper transport protein CTR1"
FT /id="PRO_0000079496"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..406
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 9..27
FT /note="1"
FT REPEAT 28..46
FT /note="2"
FT REPEAT 47..65
FT /note="3"
FT REGION 9..65
FT /note="3 X 19 AA tandem repeats of S-M-X-M-X-A-M-S-S-A-S-K-
FT T-X-X-S-X-M-X"
FT REGION 71..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 318..406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 345..406
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 344
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956"
FT MOD_RES 349
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 356
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 369
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT CARBOHYD 113
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 148
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CROSSLNK 345
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
SQ SEQUENCE 406 AA; 44442 MW; 569C9CB19C1117E8 CRC64;
MEGMNMGSSM NMDAMSSASK TVASSMASMS MDAMSSASKT ILSSMSSMSM EAMSSASKTL
ASTMSSMASM SMGSSSMSGM SMSMSSTPTS SASAQTTSDS SMSGMSGMSS SDNSSSSGMD
MDMSMGMNYY LTPTYKNYPV LFHHLHANNS GKAFGIFLLF VVAAFVYKLL LFVSWCLEVH
WFKKWDKQNK YSTLPSANSK DEGKHYDTEN NFEIQGLPKL PNLLSDIFVP SLMDLFHDII
RAFLVFTSTM IIYMLMLATM SFVLTYVFAV ITGLALSEVF FNRCKIAMLK RWDIQREIQK
AKSCPGFGNC QCGRHPEPSP DPIAVADTTS GSDQSTRLEK NNESKVAISE NNQKKTPTQE
EGCNCATDSG KNQANIERDI LENSKLQEQS GNMDQNLLPA EKFTHN
