CTR2_RAT
ID CTR2_RAT Reviewed; 657 AA.
AC B5D5N9; B5D5P0;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Cationic amino acid transporter 2;
DE Short=CAT-2;
DE Short=CAT2;
DE AltName: Full=Low affinity cationic amino acid transporter 2;
DE AltName: Full=Solute carrier family 7 member 2;
GN Name=Slc7a2; Synonyms=Atrc2, Cat2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=Wistar; TISSUE=Aortic smooth muscle;
RA Cui Z., Kelly J., Brzeski H., Marber M., Pearson J.D., Baydoun A.R.;
RT "Rattus cationic amino acid transporter-2 (rCAT-2) mRNA.";
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-645, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Functions as permease involved in the transport of the
CC cationic amino acids (arginine, lysine and ornithine). The affinity for
CC its substrates differs between isoforms created by alternative
CC splicing. May play a role in classical or alternative activation of
CC macrophages via its role in arginine transport.
CC {ECO:0000250|UniProtKB:P18581}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P18581};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:P18581}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=CAT2A;
CC IsoId=B5D5N9-1; Sequence=Displayed;
CC Name=2; Synonyms=CAT2B;
CC IsoId=B5D5N9-2; Sequence=VSP_037355;
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.
CC {ECO:0000305}.
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DR EMBL; AF245001; AAQ14243.1; -; mRNA.
DR EMBL; AF245002; AAQ14244.1; -; mRNA.
DR EMBL; CH473995; EDL78824.1; -; Genomic_DNA.
DR EMBL; CH473995; EDL78825.1; -; Genomic_DNA.
DR RefSeq; NP_001128158.1; NM_001134686.2. [B5D5N9-2]
DR RefSeq; NP_072141.2; NM_022619.3. [B5D5N9-1]
DR AlphaFoldDB; B5D5N9; -.
DR SMR; B5D5N9; -.
DR BioGRID; 249135; 1.
DR IntAct; B5D5N9; 1.
DR MINT; B5D5N9; -.
DR STRING; 10116.ENSRNOP00000055811; -.
DR GlyGen; B5D5N9; 3 sites.
DR iPTMnet; B5D5N9; -.
DR PhosphoSitePlus; B5D5N9; -.
DR jPOST; B5D5N9; -.
DR PeptideAtlas; B5D5N9; -.
DR Ensembl; ENSRNOT00000014809; ENSRNOP00000014809; ENSRNOG00000011016. [B5D5N9-2]
DR Ensembl; ENSRNOT00000015127; ENSRNOP00000015127; ENSRNOG00000011016. [B5D5N9-1]
DR GeneID; 64554; -.
DR KEGG; rno:64554; -.
DR CTD; 6542; -.
DR RGD; 68387; Slc7a2.
DR eggNOG; KOG1286; Eukaryota.
DR GeneTree; ENSGT00940000160440; -.
DR HOGENOM; CLU_007946_15_7_1; -.
DR InParanoid; B5D5N9; -.
DR OMA; WFAKTHP; -.
DR OrthoDB; 439017at2759; -.
DR PhylomeDB; B5D5N9; -.
DR Reactome; R-RNO-352230; Amino acid transport across the plasma membrane.
DR PRO; PR:B5D5N9; -.
DR Proteomes; UP000002494; Chromosome 16.
DR Proteomes; UP000234681; Chromosome 16.
DR Bgee; ENSRNOG00000011016; Expressed in liver and 17 other tissues.
DR GO; GO:0030054; C:cell junction; IEA:Ensembl.
DR GO; GO:0016021; C:integral component of membrane; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; ISO:RGD.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; ISO:RGD.
DR GO; GO:0061459; F:L-arginine transmembrane transporter activity; IDA:RGD.
DR GO; GO:0015189; F:L-lysine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0000064; F:L-ornithine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0089718; P:amino acid import across plasma membrane; ISO:RGD.
DR GO; GO:0006865; P:amino acid transport; IBA:GO_Central.
DR GO; GO:1902475; P:L-alpha-amino acid transmembrane transport; ISO:RGD.
DR GO; GO:0015807; P:L-amino acid transport; ISO:RGD.
DR GO; GO:0097638; P:L-arginine import across plasma membrane; ISO:RGD.
DR GO; GO:1903826; P:L-arginine transmembrane transport; IDA:RGD.
DR GO; GO:1903352; P:L-ornithine transmembrane transport; IBA:GO_Central.
DR GO; GO:0042116; P:macrophage activation; ISO:RGD.
DR GO; GO:0006809; P:nitric oxide biosynthetic process; ISO:RGD.
DR GO; GO:0002537; P:nitric oxide production involved in inflammatory response; ISO:RGD.
DR GO; GO:0050727; P:regulation of inflammatory response; ISO:RGD.
DR GO; GO:0043030; P:regulation of macrophage activation; ISO:RGD.
DR InterPro; IPR002293; AA/rel_permease1.
DR InterPro; IPR004755; Cat_AA_permease.
DR InterPro; IPR029485; CAT_C.
DR Pfam; PF13520; AA_permease_2; 1.
DR Pfam; PF13906; AA_permease_C; 1.
DR TIGRFAMs; TIGR00906; 2A0303; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Amino-acid transport; Cell membrane; Glycoprotein;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..657
FT /note="Cationic amino acid transporter 2"
FT /id="PRO_0000375227"
FT TOPO_DOM 1..38
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 60..66
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 88..104
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..163
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..192
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 214..248
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 270..289
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 311..339
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 361..385
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 407..409
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 410..430
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 431..489
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 490..510
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 511..523
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 524..544
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 545..554
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 555..575
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 576..581
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 582..602
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 603..657
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 24
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P52569"
FT MOD_RES 463
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P52569"
FT MOD_RES 645
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CARBOHYD 157
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 227
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 239
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 357..397
FT /note="MFPLPRILFAMARDGLLFRFLARVSKRQSPVAATMTAGVIS -> IFPMPRV
FT IYAMAEDGLLFKCLAQINSKTKTPIIATLSSGAVA (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_037355"
SQ SEQUENCE 657 AA; 71694 MW; 313E0A05771931B7 CRC64;
MIPCRAVLTF TRCLIRRKIV TLDSLEDSKL CRCLTTMDLI ALGVGSTLGA GVYVLAGEVA
KADSGPSIVV SFLIAALASV MAGLCYAEFG ARVPKTGSAY LYTYVTVGEL WAFITGWNLI
LSYVIGTSSV ARAWSGTFDE LLNKQIGQFF KTYFKMNYTG LAEYPDFFAV CLVLLLAGLL
SFGVKESAWV NKFFTAINIL VLLFVMVAGF VKGNVANWKI SEEFLKNISA SAREPPSENG
TSIYGAGGFM PYGFTGTLAG AATCFYAFVG FDCIATTGEE VRNPQKAIPI GIVTSLLVCF
MAYFGVSAAL TLMMPYYLLD EKSPLPVAFE YVGWGPAKYV VAAGSLCALS TSLLGSMFPL
PRILFAMARD GLLFRFLARV SKRQSPVAAT MTAGVISAVM AFLFDLKALV DMMSIGTLMA
YSLVAACVLI LRYQPGLCYE QPKYTPEKDI LESCTNATSK SESQVTMLQG QGFSLRTLFN
PSALPTRQSA SLVSFLVGFL AFLIAGLSIL TTYGVQAIAR LEAWSLALLA LFLVLCAAVI
LTIWRQPQNQ QKVAFMVPFL PFLPAFSILV NIYLMVQLSA DTWVRFSIWM VLGFLIYFAY
GIRHSLEGNP RDEEEDEDVC PDNVNAAAEE KSAMQANDHH QRNLSLPFIL HEKTSEC
