CTRA_BRUA2
ID CTRA_BRUA2 Reviewed; 232 AA.
AC Q2YQA4;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Cell cycle response regulator CtrA;
DE AltName: Full=Cell cycle transcriptional regulator A;
GN Name=ctrA {ECO:0000303|PubMed:26124143}; OrderedLocusNames=BAB1_1614;
OS Brucella abortus (strain 2308).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=359391;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2308;
RX PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL Infect. Immun. 73:8353-8361(2005).
RN [2]
RP TRANSCRIPTIONAL CONTROL OF CCRM.
RX PubMed=10852881; DOI=10.1128/jb.182.12.3482-3489.2000;
RA Robertson G.T., Reisenauer A., Wright R., Jensen R.B., Jensen A.,
RA Shapiro L., Roop R.M. II;
RT "The Brucella abortus CcrM DNA methyltransferase is essential for
RT viability, and its overexpression attenuates intracellular replication in
RT murine macrophages.";
RL J. Bacteriol. 182:3482-3489(2000).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.74 ANGSTROMS) OF 1-118 IN COMPLEX WITH CHPT,
RP FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION AT ASP-51, DISRUPTION
RP PHENOTYPE, INTERACTION WITH CHPT, AND MUTAGENESIS OF SER-15 AND VAL-148.
RC STRAIN=2308;
RX PubMed=26124143; DOI=10.1073/pnas.1503118112;
RA Willett J.W., Herrou J., Briegel A., Rotskoff G., Crosson S.;
RT "Structural asymmetry in a conserved signaling system that regulates
RT division, replication, and virulence of an intracellular pathogen.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:E3709-E3718(2015).
CC -!- FUNCTION: Component of a regulatory phosphorelay system that controls
CC B.abortus cell growth, division, and intracellular survival inside
CC mammalian host cells. This signaling pathway is composed of CckA, ChpT,
CC CtrA and CpdR. CtrA is a response regulator substrate of ChpT
CC (PubMed:26124143). When phosphorylated, directly regulates the
CC expression of ccrM (PubMed:10852881). Is also probably involved in the
CC transcriptional regulation of rpoD, pleC, minC and ftsE genes (By
CC similarity). {ECO:0000250|UniProtKB:Q9ZHS1,
CC ECO:0000269|PubMed:26124143, ECO:0000305|PubMed:10852881}.
CC -!- SUBUNIT: Forms an asymmetric heterotetramer with ChpT (2:2). There are
CC at least two modes of interaction between ChpT and CtrA, only one of
CC which is competent to catalyze His-Asp phosphoryl transfer.
CC {ECO:0000269|PubMed:26124143}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:26124143}.
CC -!- PTM: Is phosphorylated by ChpT-P, likely on Asp-51.
CC {ECO:0000269|PubMed:26124143}.
CC -!- DISRUPTION PHENOTYPE: This gene cannot be deleted or disrupted,
CC indicating this gene is essential in B.abortus.
CC {ECO:0000269|PubMed:26124143}.
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DR EMBL; AM040264; CAJ11570.1; -; Genomic_DNA.
DR RefSeq; WP_002964699.1; NZ_KN046823.1.
DR PDB; 4QPJ; X-ray; 2.74 A; C/D=1-118.
DR PDBsum; 4QPJ; -.
DR AlphaFoldDB; Q2YQA4; -.
DR SMR; Q2YQA4; -.
DR STRING; 359391.BAB1_1614; -.
DR EnsemblBacteria; CAJ11570; CAJ11570; BAB1_1614.
DR GeneID; 45124914; -.
DR GeneID; 55591229; -.
DR KEGG; bmf:BAB1_1614; -.
DR PATRIC; fig|359391.11.peg.1064; -.
DR HOGENOM; CLU_000445_30_1_5; -.
DR OMA; QLWGYPP; -.
DR PhylomeDB; Q2YQA4; -.
DR Proteomes; UP000002719; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IDA:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation; Two-component regulatory system.
FT CHAIN 1..232
FT /note="Cell cycle response regulator CtrA"
FT /id="PRO_0000363196"
FT DOMAIN 2..116
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 124..223
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 51
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169,
FT ECO:0000305|PubMed:26124143"
FT MUTAGEN 15
FT /note="S->R: 90% reduction in phosphoryl transfer from
FT CckA-P to CtrA via ChpT."
FT /evidence="ECO:0000269|PubMed:26124143"
FT MUTAGEN 148
FT /note="V->F: Temperature sensitive mutant that displays no
FT growth defect at 30 degrees Celsius but fails to replicate
FT when grown at 37 degrees Celsius. These mutant cells
FT cultured at the nonpermissive temperature are abnormally
FT elongated and show defects in cell divison by 24 hours,
FT with apparent budding from the midcell position.
FT Significant reduction in intracellular B.abortus
FT replication and/or survival in human macrophages."
FT /evidence="ECO:0000269|PubMed:26124143"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:4QPJ"
FT HELIX 10..21
FT /evidence="ECO:0007829|PDB:4QPJ"
FT TURN 22..24
FT /evidence="ECO:0007829|PDB:4QPJ"
FT STRAND 26..31
FT /evidence="ECO:0007829|PDB:4QPJ"
FT HELIX 33..42
FT /evidence="ECO:0007829|PDB:4QPJ"
FT STRAND 46..52
FT /evidence="ECO:0007829|PDB:4QPJ"
FT STRAND 55..57
FT /evidence="ECO:0007829|PDB:4QPJ"
FT HELIX 59..68
FT /evidence="ECO:0007829|PDB:4QPJ"
FT STRAND 75..81
FT /evidence="ECO:0007829|PDB:4QPJ"
FT HELIX 84..93
FT /evidence="ECO:0007829|PDB:4QPJ"
FT STRAND 96..102
FT /evidence="ECO:0007829|PDB:4QPJ"
FT HELIX 105..116
FT /evidence="ECO:0007829|PDB:4QPJ"
SQ SEQUENCE 232 AA; 26066 MW; 9177C2281EC76ACE CRC64;
MRVLLIEDDS AIAQSIELML KSESFNVYTT DLGEEGIDLG KLYDYDIILL DLNLPDMSGY
EVLRTLRLSK VKTPILILSG MAGIEDKVRG LGFGADDYMT KPFHKDELIA RIHAIVRRSK
GHAQSVITTG DLVVNLDAKT VEVAGQRVHL TGKEYQMLEL LSLRKGTTLT KEMFLNHLYG
GMDEPELKII DVFICKLRKK LDAVSGNQSY IETVWGRGYV LREPDAEMRE SA
