ID   CTRA_BRUAB              Reviewed;         232 AA.
AC   Q9ZHS1; Q57BS3;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Cell cycle response regulator CtrA;
DE   AltName: Full=Cell cycle transcriptional regulator A {ECO:0000303|PubMed:11929544};
GN   Name=ctrA; OrderedLocusNames=BruAb1_1586;
OS   Brucella abortus biovar 1 (strain 9-941).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=262698;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Devos D.P., Depiereux E., Letesson J.-J.;
RT   "Two-component cell cycle regulator in Brucella species.";
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS RESPONSE REGULATOR, ROLE IN
RP   CELL DIVISION, PHOSPHORYLATION AT ASP-51, DISRUPTION PHENOTYPE, AND
RP   MUTAGENESIS OF ASP-51.
RC   STRAIN=544 / Biovar 1;
RX   PubMed=11929544; DOI=10.1046/j.1365-2958.2002.02777.x;
RA   Bellefontaine A.F., Pierreux C.E., Mertens P., Vandenhaute J.,
RA   Letesson J.J., De Bolle X.;
RT   "Plasticity of a transcriptional regulation network among alpha-
RT   proteobacteria is supported by the identification of CtrA targets in
RT   Brucella abortus.";
RL   Mol. Microbiol. 43:945-960(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=9-941;
RX   PubMed=15805518; DOI=10.1128/jb.187.8.2715-2726.2005;
RA   Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z.,
RA   Li L.-L., Kapur V., Alt D.P., Olsen S.C.;
RT   "Completion of the genome sequence of Brucella abortus and comparison to
RT   the highly similar genomes of Brucella melitensis and Brucella suis.";
RL   J. Bacteriol. 187:2715-2726(2005).
CC   -!- FUNCTION: Component of a regulatory phosphorelay system that controls
CC       B.abortus cell growth, division, and intracellular survival inside
CC       mammalian host cells. This signaling pathway is composed of CckA, ChpT,
CC       CtrA and CpdR. CtrA is a response regulator substrate of ChpT (By
CC       similarity). When phosphorylated, directly regulates the expression of
CC       ccrM. Is also probably involved in the transcriptional regulation of
CC       rpoD, pleC, minC and ftsE genes (PubMed:11929544).
CC       {ECO:0000250|UniProtKB:Q2YQA4, ECO:0000269|PubMed:11929544}.
CC   -!- SUBUNIT: Forms an asymmetric heterotetramer with ChpT (2:2). There are
CC       at least two modes of interaction between ChpT and CtrA, only one of
CC       which is competent to catalyze His-Asp phosphoryl transfer.
CC       {ECO:0000250|UniProtKB:Q2YQA4}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Is phosphorylated by ChpT-P on Asp-51.
CC       {ECO:0000250|UniProtKB:Q2YQA4, ECO:0000269|PubMed:11929544}.
CC   -!- DISRUPTION PHENOTYPE: Lethal. {ECO:0000269|PubMed:11929544}.
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DR   EMBL; AF051939; AAC69920.1; -; mRNA.
DR   EMBL; AF411570; AAL86376.1; -; Genomic_DNA.
DR   EMBL; AE017223; AAX74911.1; -; Genomic_DNA.
DR   PIR; AI3304; AI3304.
DR   RefSeq; WP_002964699.1; NC_006932.1.
DR   AlphaFoldDB; Q9ZHS1; -.
DR   SMR; Q9ZHS1; -.
DR   EnsemblBacteria; AAX74911; AAX74911; BruAb1_1586.
DR   GeneID; 45124914; -.
DR   GeneID; 55591229; -.
DR   KEGG; bmb:BruAb1_1586; -.
DR   HOGENOM; CLU_000445_30_1_5; -.
DR   OMA; QLWGYPP; -.
DR   Proteomes; UP000000540; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; DNA-binding; Phosphoprotein; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..232
FT                   /note="Cell cycle response regulator CtrA"
FT                   /id="PRO_0000363197"
FT   DOMAIN          2..116
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        124..223
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         51
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169,
FT                   ECO:0000269|PubMed:11929544"
FT   MUTAGEN         51
FT                   /note="D->E: No phosphorylation."
FT                   /evidence="ECO:0000269|PubMed:11929544"
SQ   SEQUENCE   232 AA;  26066 MW;  9177C2281EC76ACE CRC64;
     MRVLLIEDDS AIAQSIELML KSESFNVYTT DLGEEGIDLG KLYDYDIILL DLNLPDMSGY
     EVLRTLRLSK VKTPILILSG MAGIEDKVRG LGFGADDYMT KPFHKDELIA RIHAIVRRSK
     GHAQSVITTG DLVVNLDAKT VEVAGQRVHL TGKEYQMLEL LSLRKGTTLT KEMFLNHLYG
     GMDEPELKII DVFICKLRKK LDAVSGNQSY IETVWGRGYV LREPDAEMRE SA