CTSRD_RAT
ID CTSRD_RAT Reviewed; 803 AA.
AC B5DFM7; A0A0H2UI41;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 23-FEB-2022, sequence version 2.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Cation channel sperm-associated auxiliary subunit delta {ECO:0000312|RGD:1594167};
DE Short=CatSper-delta;
DE Short=CatSperdelta;
DE AltName: Full=Transmembrane protein 146;
DE Flags: Precursor;
GN Name=Catsperd {ECO:0000312|RGD:1594167}; Synonyms=Tmem146;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1] {ECO:0000312|Proteomes:UP000002494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway {ECO:0000312|Proteomes:UP000002494};
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Auxiliary component of the CatSper complex, a complex
CC involved in sperm cell hyperactivation. Sperm cell hyperactivation is
CC needed for sperm motility which is essential late in the preparation of
CC sperm for fertilization. Required for CATSPER1 stability before
CC intraflagellar transport and/or incorporation of the CatSper complex
CC channel into the flagellar membrane. {ECO:0000250|UniProtKB:E9Q9F6}.
CC -!- SUBUNIT: Component of the CatSper complex or CatSpermasome composed of
CC the core pore-forming members CATSPER1, CATSPER2, CATSPER3 and CATSPER4
CC as well as auxiliary members CATSPERB, CATSPERG, CATSPERD, CATSPERE,
CC CATSPERZ, C2CD6/CATSPERT, SLCO6C1, TMEM249, TMEM262 and EFCAB9 (By
CC similarity). HSPA1 may be an additional auxiliary complex member (By
CC similarity). The core complex members CATSPER1, CATSPER2, CATSPER3 and
CC CATSPER4 form a heterotetrameric channel. The auxiliary CATSPERB,
CC CATSPERG, CATSPERD and CATSPERE subunits form a pavilion-like structure
CC over the pore which stabilizes the complex through interactions with
CC CATSPER4, CATSPER3, CATSPER1 and CATSPER2 respectively. SLCO6C1
CC interacts with CATSPERE and TMEM262/CATSPERH interacts with CATSPERB,
CC further stabilizing the complex. C2CD6/CATSPERT interacts at least with
CC CATSPERD and is required for targeting the CatSper complex in the
CC flagellar membrane (By similarity). {ECO:0000250|UniProtKB:E9Q9F6,
CC ECO:0000250|UniProtKB:Q91ZR5}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum membrane
CC {ECO:0000250|UniProtKB:E9Q9F6}; Single-pass type I membrane protein
CC {ECO:0000255}. Note=Specifically located in the principal piece of
CC sperm tail. {ECO:0000250|UniProtKB:E9Q9F6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=B5DFM7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=B5DFM7-2; Sequence=VSP_061421, VSP_061422;
CC -!- SIMILARITY: Belongs to the CATSPERD family. {ECO:0000305}.
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DR EMBL; AABR07066520; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR07066522; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR07066521; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC169121; AAI69121.1; -; mRNA.
DR RefSeq; NP_001128456.1; NM_001134984.1.
DR AlphaFoldDB; B5DFM7; -.
DR STRING; 10116.ENSRNOP00000067404; -.
DR GlyGen; B5DFM7; 2 sites.
DR PaxDb; B5DFM7; -.
DR GeneID; 680264; -.
DR KEGG; rno:680264; -.
DR CTD; 257062; -.
DR RGD; 1594167; Catsperd.
DR VEuPathDB; HostDB:ENSRNOG00000048818; -.
DR eggNOG; ENOG502QSPE; Eukaryota.
DR HOGENOM; CLU_019182_0_0_1; -.
DR InParanoid; B5DFM7; -.
DR OMA; QDNYSFI; -.
DR OrthoDB; 213907at2759; -.
DR PhylomeDB; B5DFM7; -.
DR Reactome; R-RNO-1300642; Sperm Motility And Taxes.
DR PRO; PR:B5DFM7; -.
DR Proteomes; UP000002494; Chromosome 9.
DR Bgee; ENSRNOG00000048818; Expressed in testis and 14 other tissues.
DR GO; GO:0036128; C:CatSper complex; ISS:UniProtKB.
DR GO; GO:0097228; C:sperm principal piece; ISS:UniProtKB.
DR GO; GO:0030317; P:flagellated sperm motility; ISS:UniProtKB.
DR GO; GO:0048240; P:sperm capacitation; ISS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR InterPro; IPR028751; CATSPERD/E.
DR PANTHER; PTHR33722; PTHR33722; 1.
DR Pfam; PF15020; CATSPERD; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Cell projection; Cilium;
KW Developmental protein; Differentiation; Disulfide bond; Flagellum;
KW Glycoprotein; Membrane; Reference proteome; Signal; Spermatogenesis;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..15
FT /evidence="ECO:0000255"
FT CHAIN 16..803
FT /note="Cation channel sperm-associated auxiliary subunit
FT delta"
FT /id="PRO_0000416885"
FT TOPO_DOM 16..720
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:E9Q9F6"
FT TRANSMEM 721..742
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:E9Q9F6"
FT TOPO_DOM 743..803
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:E9Q9F6"
FT REGION 782..803
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 783..803
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 226
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 418
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 436
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 468
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 534
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 545
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 626
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 19..365
FT /evidence="ECO:0000250|UniProtKB:E9Q9F6"
FT DISULFID 55..142
FT /evidence="ECO:0000250|UniProtKB:E9Q9F6"
FT DISULFID 141..148
FT /evidence="ECO:0000250|UniProtKB:E9Q9F6"
FT DISULFID 383..492
FT /evidence="ECO:0000250|UniProtKB:E9Q9F6"
FT DISULFID 506..698
FT /evidence="ECO:0000250|UniProtKB:E9Q9F6"
FT DISULFID 521..568
FT /evidence="ECO:0000250|UniProtKB:E9Q9F6"
FT DISULFID 620..648
FT /evidence="ECO:0000250|UniProtKB:E9Q9F6"
FT VAR_SEQ 696..713
FT /note="SYCDLNTIFSVYVYGALP -> RYSSTAQGSAPVLGFGAK (in isoform
FT 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_061421"
FT VAR_SEQ 714..803
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_061422"
SQ SEQUENCE 803 AA; 91436 MW; 184B06F1236145C5 CRC64;
MLMLMLAAVA TVVRAQTVCR FRTVRTGKVF ANPVTLEGDL LFYAFSNTVV VKNVCKTDIA
VYLGQRVFIT KNRFEASILP LTIPKSMEVK MPSITSAHFV SDAMILFVID GKVYSYNFIE
DIWRTVNGIT EPVSHISGDP CCFEGYFCLE LSNNLFAYFR GGQMPGTNIY FSNNGGFSFE
LLNSDRMSHL KGLLGGIFHF HSLSQVGILL VENNLGTFHY LEYPLNHSTG VPFLYESPLE
VIIKPQQRGF LILWNQKTLL VSSNSGQIVE AMQLMEEGNI NDLNVEHAKL TIHSIASNTY
ELAFLVEQDQ LYYGSQSYMG NYIIKLSNQQ FWSEEASVHF WDVGMLEVLT PVSDPYFPAF
DFKKCLVNVQ LALMDQSLQL EPCNVEFLES TMEDRMFIID MNSKLKLSAL MVPRKGMNPT
PLVMVSNPHA LGFKANLTQF GNMYDGNSKF KLDIELQQQQ HWGNSELNFT ASIKHEAISS
ITVDIADKTL SCVDLKPLST LISVGCDLTK KVIVQNKISA CAMGILDPVL LQKNYSYTIE
KEAYNPTSYS GEAQDDLIVF YQYKELGCPR LVYYDKPWKP VVELWKDGTL EEIMNAEYVI
LELNGIVTYS YSLTAATAHC RSQPQNWSIF KEDAEKPSLW NRETYVSCHE DNQDNPLLWP
NVEYQILGGR TDNKIIFGQR NGIYTFYLTV VDPYYSYCDL NTIFSVYVYG ALPVAEFRPM
TSILLMVTVT LFTMWLAYAI PKQLRTERGR RLTGFCFQIF KYCPGICTCA WLRGKMRRGL
RSRRVKDQPE KIPQIGKKPD IKK
