CTSRT_MOUSE
ID CTSRT_MOUSE Reviewed; 2282 AA.
AC A0A5F8MPU3; E9Q152;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 11-DEC-2019, sequence version 1.
DT 03-AUG-2022, entry version 9.
DE RecName: Full=Cation channel sperm-associated targeting subunit tau {ECO:0000305|PubMed:34998468};
DE Short=CatSper-tau {ECO:0000303|PubMed:34998468};
DE AltName: Full=C2 calcium-dependent domain-containing 6;
GN Name=C2cd6 {ECO:0000312|MGI:MGI:1920713};
GN Synonyms=Als2cr11 {ECO:0000312|MGI:MGI:1920713},
GN CATSPERT {ECO:0000303|PubMed:34998468};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|Proteomes:UP000000589};
RN [1] {ECO:0000312|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, DISRUPTION PHENOTYPE, AND TISSUE
RP SPECIFICITY.
RX PubMed=34998468; DOI=10.1016/j.celrep.2021.110226;
RA Hwang J.Y., Wang H., Lu Y., Ikawa M., Chung J.J.;
RT "C2cd6-encoded CatSpertau targets sperm calcium channel to Ca2+ signaling
RT domains in the flagellar membrane.";
RL Cell Rep. 38:110226-110226(2022).
CC -!- FUNCTION: Auxiliary component of the CatSper complex, a complex
CC involved in sperm cell hyperactivation. Sperm cell hyperactivation is
CC needed for sperm motility which is essential late in the preparation of
CC sperm for fertilization. Required for CatSper complex targeting and
CC trafficking into the quadrilinear nanodomains. Targets the preassembled
CC CatSper complexes to elongating flagella, where it links the channel-
CC carrying vesicles and motor proteins. {ECO:0000269|PubMed:34998468}.
CC -!- SUBUNIT: Component of the CatSper complex or CatSpermasome composed of
CC the core pore-forming members CATSPER1, CATSPER2, CATSPER3 and CATSPER4
CC as well as auxiliary members CATSPERB, CATSPERG, CATSPERD, CATSPERE,
CC CATSPERZ, C2CD6/CATSPERT, SLCO6C1, TMEM249, TMEM262 and EFCAB9. HSPA1
CC may be an additional auxiliary complex member. The core complex members
CC CATSPER1, CATSPER2, CATSPER3 and CATSPER4 form a heterotetrameric
CC channel. The auxiliary CATSPERB, CATSPERG, CATSPERD and CATSPERE
CC subunits form a pavilion-like structure over the pore which stabilizes
CC the complex through interactions with CATSPER4, CATSPER3, CATSPER1 and
CC CATSPER2 respectively. SLCO6C1 interacts with CATSPERE and
CC TMEM262/CATSPERH interacts with CATSPERB, further stabilizing the
CC complex. C2CD6/CATSPERT interacts at least with CATSPERD and is
CC required for targeting the CatSper complex in the flagellar membrane.
CC {ECO:0000269|PubMed:34998468}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum membrane
CC {ECO:0000269|PubMed:34998468}. Note=Specifically located in the
CC principal piece of the sperm tail. {ECO:0000269|PubMed:34998468}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=L {ECO:0000303|PubMed:34998468};
CC IsoId=A0A5F8MPU3-1; Sequence=Displayed;
CC Name=2; Synonyms=S {ECO:0000303|PubMed:34998468};
CC IsoId=A0A5F8MPU3-2; Sequence=VSP_061603, VSP_061604;
CC -!- TISSUE SPECIFICITY: [Isoform 1]: Expressed in cauda sperm (at protein
CC level). {ECO:0000269|PubMed:34998468}.
CC -!- TISSUE SPECIFICITY: [Isoform 2]: Expressed in cauda sperm (at protein
CC level). {ECO:0000269|PubMed:34998468}.
CC -!- DISRUPTION PHENOTYPE: Mutant mice have no gross abnormality in
CC survival, appearance or behavior. Males are infertile with defective
CC sperm hyperactivation. Females have normal reproductive ability and
CC give birth with no difference in litter size.
CC {ECO:0000269|PubMed:34998468}.
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DR RefSeq; NP_080876.1; NM_026600.1.
DR RefSeq; NP_780409.2; NM_175200.4.
DR AlphaFoldDB; A0A5F8MPU3; -.
DR SMR; A0A5F8MPU3; -.
DR PaxDb; E9Q152; -.
DR PeptideAtlas; E9Q152; -.
DR PRIDE; A0A5F8MPU3; -.
DR ProteomicsDB; 363953; -.
DR Antibodypedia; 35141; 161 antibodies from 22 providers.
DR Ensembl; ENSMUST00000238949.2; ENSMUSP00000159118.2; ENSMUSG00000072295.8.
DR GeneID; 73463; -.
DR UCSC; uc007bdc.1; mouse.
DR CTD; 151254; -.
DR MGI; MGI:1920713; C2cd6.
DR VEuPathDB; HostDB:ENSMUSG00000072295; -.
DR eggNOG; ENOG502S23V; Eukaryota.
DR GeneTree; ENSGT00390000018209; -.
DR HOGENOM; CLU_030502_1_0_1; -.
DR InParanoid; E9Q152; -.
DR OMA; HVQHYLV; -.
DR OrthoDB; 536178at2759; -.
DR TreeFam; TF328407; -.
DR BioGRID-ORCS; 73463; 1 hit in 72 CRISPR screens.
DR ChiTaRS; C2cd6; mouse.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; E9Q152; protein.
DR Bgee; ENSMUSG00000072295; Expressed in spermatid and 14 other tissues.
DR ExpressionAtlas; A0A5F8MPU3; baseline and differential.
DR InterPro; IPR031462; C2CD6.
DR PANTHER; PTHR21665; PTHR21665; 2.
DR Pfam; PF15729; ALS2CR11; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cell projection; Cilium; Coiled coil;
KW Flagellum; Membrane; Reference proteome.
FT CHAIN 1..2282
FT /note="Cation channel sperm-associated targeting subunit
FT tau"
FT /id="PRO_0000456295"
FT DOMAIN 131..266
FT /note="C2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT VAR_SEQ 534..557
FT /note="NRRKMEKSPHFSDVLIIPDKPFED -> VKLRHLPISPERIRRRNLGFSPAE
FT (in isoform 2)"
FT /id="VSP_061603"
FT VAR_SEQ 558..2282
FT /note="Missing (in isoform 2)"
FT /id="VSP_061604"
SQ SEQUENCE 2282 AA; 257620 MW; 487BC91E37580446 CRC64;
MELPPPGNRR VSINNPQETS GRVPTTSAGF PTQSSKISLK RSTYAYRPSM MSNRSSGGQS
LLPSSILQKT SLNPPGSLQS KPSNLSSVHY ADEEGKPLTD KNKDKDKGRG KGKGTGTRLL
TMLRKTLQGS QSDEMAIANQ TPNLIPFGDV VGCLAIHIKS CRQFSQRFLG QQRFNLFMRV
SINNIVKCTK IRHLKAVNNE KNLVLRFGEM KYFSVQVPRR QDDERNFIYL ELMHDGGDPE
SPPIPLGGAE SHLYEVIQKG CFTEVMHLMH KNSSICRVEV EFMFSYGNFG YGFSHQLKPL
QKAIEPSMFM NIAPPPERTD PVTNVITPQR VEYPAFLSPE FNVSIGVPEA SHATVVQLEK
LREKPRERLE RMKEEYKNMS TWIEKADYLR NLINPKMTKR DNKGSSIPSE SNSSALEELE
RTTYDIHHRK YEAISNEYGD KEGRVIPVLK VLDQNYSEVF LPKSSDSTPL EDVLLPPIHS
LQIVEENEMP HLPKTSEPED RPHEERKSIV FSSDEELMPK HPSILKISSS QQENRRKMEK
SPHFSDVLII PDKPFEDLNT NKKGRPPIEL RKSWERHPTD VACSLRKVAF AQKDYTIPVC
KAETTEFKPK HQFQKLSKSG LDPFLRNINS KMSFRKKKDH DDGYRNLSTS SAEILEHEDQ
DPPYPGHSGS AGSDATWAEN PSPVTVQMVN RDSLPPDLIT ATIVISDRKN KLSLDSVFNS
ANSLNTKSIF ASDNPVVSLT KLSDSDNKLI TDSSFNTTKP SNRRLSKDSN FNTTKPSDRK
LSSDPSSNTT KPSDTKLFSD PSSNKLSQGP SSNASQLSGS NRLSHNPSIN GNKSSYTSDL
NKVSRDPSIV STISSDPNKL SRDPSFVLAK SSDPNKLSHY PSIISAKSSD PNKLSHDPSF
VSARSSDPNK LSHDPSIISA RSSDPIKLSR DPSFVSARSS DPNKLSHDPS IISARSSDPN
KLSRDPSINS TKLSDPSKLS RDPSIFSTKS SDPNKLYRVP SIISGMSSNP KLSRDPSIIS
GMSSDPKLSR DPSIISAKSS DPNKLSHDPS IISGMSSNPN KLSHDPSIIS MKLSDMSKLS
RESSINASKS SDTNQLSYDP NIISAKSLDS NNSSASSSPT VNSDTTTNAA EPSGTKNMLD
PVVSTIDSSD KQSKLEWLPN VQGASSVTEN INTHRSSNSV NFTSDIDILK QSIVLKSILS
KNLQDLSDEL FSKSELYTND EGYSPPPSVH SRPSDSTDDR VLGKVQDLNS WRSSKDLLNS
QVLLSPVVKN SPQDLLPEGE PGKSSDIEDY VSEKLLEAAG RNFPMNRKSS FKKKHLVSEE
SSSEHVLSGS IYEYVIKQIF TAPIFSQLGI GIKSSSEARM DSQNQLLTPW ERSVTSHIIN
YEEKDSDVNL SQSKSIISQI IQSFPVNTLL ESGVIKVIEL DKEHQNSLLD SQTTSSTEQY
SDSRSQIKLL SRQNTSSINP LDSSVSGAEY TEDCQSISTQ ESKYPVRDTK SDSPNDTEEM
ELDSNLESSS SSLDKVKDTD TAKLKNILKN IFSIFFKYNQ SERRQQPEKD SESLIKHSSS
SGSEHLEKTQ ENFNKADKKV DRKPILNPKL RMFLEKLSET EVKNLKSELS KHIQHYLVER
LTESGHITKE DLPTIYHKLY LMNEKVELKE QTPFQEKYSE TVKEIMSFVN NFNHHFIDKH
LETKLRGFLS EILQNYFLKN LSVSNLFNET DAMALHASMS PVRSKSELGQ DIADGNFGSS
LKINMQYPVT KSLQHYLQDL SENELLSLKT DLSKYLQVLF IEKLYKSGLV SERQLKGISQ
EIISLHSPSI PLKHIKTNLP FRNESYFMRE DSEEQMKYLK NGQNAALHVL LKDKCGETEL
SRKKERESSF SQILKENLPA IWEQKNIYTR EEETLNLIQM QSFLNKNNQA NPLTRSPERP
SDISLKKQKK DHGFMQFTQV EGSVYKTEIQ DPYSWDSRSK TIQSKPCLEK TLKMKLLDKR
ENNNFYKLTA QEKLDTEFSS YLKLPNCKIP KEKEPISRLS FPTWKTNTFI HVKPEIGEQS
KLDHYYQRLK GNNNNNKKHL VTFAQFKNEM ETLYRNPYEA CNEKRAKISE SQSFKYKEKE
KSSRPFFFPE VLKRENTKSK RKERDHATKP KKSFHKIVRL LPATLPTTRP HLRKSAPRNL
LHWTARRTIH DCLDRFDDLH APTVKCPKKS KSGARLLGKS PEDSHNQAKH CARPYTAPEP
NKRRESAAWK FASPRMVSAG LLHLYVTPAY GIRKVRSKRK LKEDIEKRPL ISEIIQMLDN
AE
