CTSRZ_HUMAN
ID CTSRZ_HUMAN Reviewed; 200 AA.
AC Q9NTU4;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Cation channel sperm-associated auxiliary subunit zeta {ECO:0000312|HGNC:HGNC:19231};
DE Short=CatSper-zeta {ECO:0000250|UniProtKB:Q9CQP8};
DE Short=CatSperzeta {ECO:0000250|UniProtKB:Q9CQP8};
DE AltName: Full=Testis-expressed protein 40 {ECO:0000312|HGNC:HGNC:19231};
GN Name=CATSPERZ {ECO:0000312|HGNC:HGNC:19231};
GN Synonyms=C11orf20 {ECO:0000312|HGNC:HGNC:19231},
GN TEX40 {ECO:0000312|HGNC:HGNC:19231};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [2]
RP IDENTIFICATION.
RX PubMed=19139490; DOI=10.1074/mcp.m800504-mcp200;
RA Jia W., Lu Z., Fu Y., Wang H.P., Wang L.H., Chi H., Yuan Z.F., Zheng Z.B.,
RA Song L.N., Han H.H., Liang Y.M., Wang J.L., Cai Y., Zhang Y.K., Deng Y.L.,
RA Ying W.T., He S.M., Qian X.H.;
RT "A strategy for precise and large scale identification of core fucosylated
RT glycoproteins.";
RL Mol. Cell. Proteomics 8:913-923(2009).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=28226241; DOI=10.7554/elife.23082;
RA Chung J.J., Miki K., Kim D., Shim S.H., Shi H.F., Hwang J.Y., Cai X.,
RA Iseri Y., Zhuang X., Clapham D.E.;
RT "CatSperzeta regulates the structural continuity of sperm Ca(2+) signaling
RT domains and is required for normal fertility.";
RL Elife 6:0-0(2017).
CC -!- FUNCTION: Auxiliary component of the CatSper complex, a complex
CC involved in sperm cell hyperactivation. Sperm cell hyperactivation is
CC needed for sperm motility which is essential late in the preparation of
CC sperm for fertilization. Required for a distribution of the CatSper
CC complex in linear quadrilateral nanodomains along the flagellum,
CC maximizing fertilization inside the mammalian female reproductive
CC tract. Together with EFCAB9, associates with the CatSper channel pore
CC and is required for the two-row structure of each single CatSper
CC channel. {ECO:0000250|UniProtKB:Q9CQP8}.
CC -!- SUBUNIT: Component of the CatSper complex or CatSpermasome composed of
CC the core pore-forming members CATSPER1, CATSPER2, CATSPER3 and CATSPER4
CC as well as auxiliary members CATSPERB, CATSPERG, CATSPERD, CATSPERE,
CC CATSPERZ, C2CD6/CATSPERT, TMEM249, TMEM262 and EFCAB9 (By similarity).
CC HSPA1 may be an additional auxiliary complex member (By similarity).
CC The core complex members CATSPER1, CATSPER2, CATSPER3 and CATSPER4 form
CC a heterotetrameric channel (By similarity). The auxiliary CATSPERB,
CC CATSPERG, CATSPERD and CATSPERE subunits form a pavilion-like structure
CC over the pore which stabilizes the complex through interactions with
CC CATSPER4, CATSPER3, CATSPER1 and CATSPER2 respectively (By similarity).
CC TMEM262/CATSPERH interacts with CATSPERB, further stabilizing the
CC complex. C2CD6/CATSPERT interacts at least with CATSPERD and is
CC required for targeting the CatSper complex in the flagellar membrane
CC (By similarity). Interacts with EFCAB9; the interaction is direct,
CC Ca(2+)-dependent and connects EFCAB9 with the CatSper complex (By
CC similarity). Dissociates from EFCAB9 at elevated pH (By similarity).
CC {ECO:0000250|UniProtKB:Q91ZR5, ECO:0000250|UniProtKB:Q9CQP8}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum membrane
CC {ECO:0000269|PubMed:28226241}; Peripheral membrane protein
CC {ECO:0000305}. Note=Specifically located in the principal piece of
CC sperm tail (PubMed:28226241). Although it does not contain a
CC transmembrane domain, localizes with the CatSper complex at the
CC flagellum membrane (PubMed:28226241). {ECO:0000269|PubMed:28226241}.
CC -!- CAUTION: A report observed N-glycosylation at Asn-58 (PubMed:19139490).
CC However, as the protein is not predicted to localize in an
CC extracellular compartment of the cell, additional evidence is required
CC to confirm this result. {ECO:0000305|PubMed:19139490}.
CC -!- CAUTION: In mouse, Slco6c1 is an additional auxiliary subunit of the
CC CatSper complex. It is unclear if the related SLCO6A1 protein performs
CC the same role in non-rodent species. {ECO:0000305}.
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DR EMBL; AL117564; CAB55995.2; -; mRNA.
DR PIR; T17305; T17305.
DR RefSeq; NP_001034585.1; NM_001039496.1.
DR AlphaFoldDB; Q9NTU4; -.
DR SMR; Q9NTU4; -.
DR BioGRID; 307941; 3.
DR STRING; 9606.ENSP00000443917; -.
DR TCDB; 1.A.1.19.1; the voltage-gated ion channel (vic) superfamily.
DR iPTMnet; Q9NTU4; -.
DR PhosphoSitePlus; Q9NTU4; -.
DR BioMuta; CATSPERZ; -.
DR DMDM; 74719331; -.
DR MassIVE; Q9NTU4; -.
DR PaxDb; Q9NTU4; -.
DR PeptideAtlas; Q9NTU4; -.
DR PRIDE; Q9NTU4; -.
DR ProteomicsDB; 82631; -.
DR Antibodypedia; 66419; 35 antibodies from 12 providers.
DR DNASU; 25858; -.
DR Ensembl; ENST00000328404.8; ENSP00000491717.1; ENSG00000219435.6.
DR GeneID; 25858; -.
DR KEGG; hsa:25858; -.
DR MANE-Select; ENST00000328404.8; ENSP00000491717.1; NM_001039496.2; NP_001034585.1.
DR CTD; 25858; -.
DR DisGeNET; 25858; -.
DR GeneCards; CATSPERZ; -.
DR HGNC; HGNC:19231; CATSPERZ.
DR HPA; ENSG00000219435; Tissue enriched (testis).
DR MIM; 617511; gene.
DR neXtProt; NX_Q9NTU4; -.
DR OpenTargets; ENSG00000219435; -.
DR PharmGKB; PA164716726; -.
DR VEuPathDB; HostDB:ENSG00000219435; -.
DR eggNOG; ENOG502RU31; Eukaryota.
DR GeneTree; ENSGT00400000022853; -.
DR InParanoid; Q9NTU4; -.
DR OMA; EMDEHAL; -.
DR OrthoDB; 1360720at2759; -.
DR PhylomeDB; Q9NTU4; -.
DR PathwayCommons; Q9NTU4; -.
DR SignaLink; Q9NTU4; -.
DR BioGRID-ORCS; 25858; 10 hits in 219 CRISPR screens.
DR Pharos; Q9NTU4; Tdark.
DR PRO; PR:Q9NTU4; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q9NTU4; protein.
DR Bgee; ENSG00000219435; Expressed in left testis and 92 other tissues.
DR ExpressionAtlas; Q9NTU4; baseline.
DR GO; GO:0036128; C:CatSper complex; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:LIFEdb.
DR GO; GO:0097228; C:sperm principal piece; IDA:UniProtKB.
DR GO; GO:0030317; P:flagellated sperm motility; ISS:UniProtKB.
DR GO; GO:0007140; P:male meiotic nuclear division; ISS:UniProtKB.
DR GO; GO:0048240; P:sperm capacitation; ISS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR InterPro; IPR039019; CATSPERZ.
DR PANTHER; PTHR42155; PTHR42155; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cell projection; Cilium; Differentiation; Flagellum;
KW Membrane; Reference proteome; Spermatogenesis.
FT CHAIN 1..200
FT /note="Cation channel sperm-associated auxiliary subunit
FT zeta"
FT /id="PRO_0000349322"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 58..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..27
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 68
FT /note="P -> L (in dbSNP:rs2286614)"
FT /id="VAR_046370"
SQ SEQUENCE 200 AA; 22839 MW; 25F6EC8873330DCB CRC64;
MEEKPSKVSL KSSDRQGSDE ESVHSDTRDL WTTTTLSQAQ LNMPLSEVCE GFDEEGRNIS
KTRGWHSPGR GSLDEGYKAS HKPEELDEHA LVELELHRGS SMEINLGEKD TASQIEAEKS
SSMSSLNIAK HMPHRAYWAE QQSRLPLPLM ELMENEALEI LTKALRSYQL GIGRDHFLTK
ELQRYIEGLK KRRSKRLYVN
