ID   CTU1_DANRE              Reviewed;         343 AA.
AC   Q803X1; A8WGP6; A9JRT6;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 1 {ECO:0000255|HAMAP-Rule:MF_03053};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_03053};
DE   AltName: Full=ATP-binding domain-containing protein 3 {ECO:0000255|HAMAP-Rule:MF_03053};
DE   AltName: Full=Cytoplasmic tRNA adenylyltransferase 1 {ECO:0000255|HAMAP-Rule:MF_03053};
GN   Name=ctu1; Synonyms=atpbd3, ncs6; ORFNames=zgc:55395;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB; TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds
CC       tRNAs and probably acts by catalyzing adenylation of tRNAs, an
CC       intermediate required for 2-thiolation. It is unclear whether it acts
CC       as a sulfurtransferase that transfers sulfur from thiocarboxylated urm1
CC       onto the uridine of tRNAs at wobble position. {ECO:0000255|HAMAP-
CC       Rule:MF_03053}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03053}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03053}.
CC   -!- SIMILARITY: Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_03053}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC044158; AAH44158.1; -; mRNA.
DR   EMBL; BC155784; AAI55785.1; -; mRNA.
DR   EMBL; BC154793; AAI54794.1; -; mRNA.
DR   RefSeq; NP_956424.1; NM_200130.1.
DR   AlphaFoldDB; Q803X1; -.
DR   SMR; Q803X1; -.
DR   STRING; 7955.ENSDARP00000009303; -.
DR   PaxDb; Q803X1; -.
DR   Ensembl; ENSDART00000017850; ENSDARP00000009303; ENSDARG00000020986.
DR   Ensembl; ENSDART00000164426; ENSDARP00000134794; ENSDARG00000020986.
DR   GeneID; 393098; -.
DR   KEGG; dre:393098; -.
DR   CTD; 90353; -.
DR   ZFIN; ZDB-GENE-040426-704; ctu1.
DR   eggNOG; KOG2840; Eukaryota.
DR   GeneTree; ENSGT00390000001041; -.
DR   HOGENOM; CLU_026481_1_2_1; -.
DR   InParanoid; Q803X1; -.
DR   OrthoDB; 551308at2759; -.
DR   PhylomeDB; Q803X1; -.
DR   TreeFam; TF352405; -.
DR   UniPathway; UPA00988; -.
DR   PRO; PR:Q803X1; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 8.
DR   Bgee; ENSDARG00000020986; Expressed in mature ovarian follicle and 25 other tissues.
DR   ExpressionAtlas; Q803X1; baseline and differential.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0002144; C:cytosolic tRNA wobble base thiouridylase complex; IBA:GO_Central.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; ISS:UniProtKB.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03053; CTU1; 1.
DR   InterPro; IPR032442; CTU1_C.
DR   InterPro; IPR000541; Ncs6/Tuc1/Ctu1.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR011063; TilS/TtcA_N.
DR   InterPro; IPR035107; tRNA_thiolation_TtcA_Ctu1.
DR   InterPro; IPR020554; UPF0021_CS.
DR   PANTHER; PTHR11807:SF12; PTHR11807:SF12; 1.
DR   Pfam; PF01171; ATP_bind_3; 1.
DR   Pfam; PF16503; zn-ribbon_14; 1.
DR   PIRSF; PIRSF004976; ATPase_YdaO; 1.
DR   TIGRFAMs; TIGR00269; TIGR00269; 1.
DR   PROSITE; PS01263; UPF0021; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; RNA-binding; Transferase; tRNA processing;
KW   tRNA-binding.
FT   CHAIN           1..343
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 1"
FT                   /id="PRO_0000282393"
FT   CONFLICT        70
FT                   /note="M -> I (in Ref. 1; AAI54794)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   343 AA;  38401 MW;  E059805C95D673B5 CRC64;
     MPVQCSNCEQ KRAVLKRPKT GHSLCKDCFF WAFEEEIHQT ITSAGLFNRG ETVAIGASGG
     KDSTVLAHVM KLLNERYDYG LKLLLLSVDE GITGYRDDSL ETVKRNQQQY ELPLKIVSYE
     ELYGWTMDAI VKQVGLKNNC TFCGVFRRQA LDRGAMMLNV DKICTGHNAD DVAETVLMNV
     LRGDIARLRR CTAISTSSEG DGAIPRCKPL KYAYEKEIVL YAYFKKLDYF STECIYSPNA
     YRGHARAFLK DLESVRPSAI IDVIHSGETL SVKEGVKMPV QGTCSRCGYI SSQALCKSCV
     LLEGLNRGLP KLGIGKHHRL HGKILAQEPL TEQEERKLKA VDF