CTU1_DICDI
ID CTU1_DICDI Reviewed; 360 AA.
AC Q94480; Q54RL9;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 1 {ECO:0000255|HAMAP-Rule:MF_03053};
DE EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_03053};
DE AltName: Full=ATP-binding domain-containing protein 3 {ECO:0000255|HAMAP-Rule:MF_03053};
DE AltName: Full=Cytoplasmic tRNA adenylyltransferase 1 {ECO:0000255|HAMAP-Rule:MF_03053};
GN Name=ctu1; Synonyms=atpbd3, rcdM, veg136; ORFNames=DDB_G0282921;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-360.
RC STRAIN=AX4;
RX PubMed=8643615; DOI=10.1073/pnas.93.11.5562;
RA Kuspa A., Loomis W.F.;
RT "Ordered yeast artificial chromosome clones representing the Dictyostelium
RT discoideum genome.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:5562-5566(1996).
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds
CC tRNAs and probably acts by catalyzing adenylation of tRNAs, an
CC intermediate required for 2-thiolation. It is unclear whether it acts
CC as a sulfurtransferase that transfers sulfur from thiocarboxylated urm1
CC onto the uridine of tRNAs at wobble position. {ECO:0000255|HAMAP-
CC Rule:MF_03053}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03053}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03053}.
CC -!- SIMILARITY: Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_03053}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAFI02000049; EAL65904.1; -; Genomic_DNA.
DR EMBL; U66527; AAB06790.1; -; mRNA.
DR RefSeq; XP_639332.1; XM_634240.1.
DR AlphaFoldDB; Q94480; -.
DR SMR; Q94480; -.
DR STRING; 44689.DDB0191478; -.
DR PaxDb; Q94480; -.
DR EnsemblProtists; EAL65904; EAL65904; DDB_G0282921.
DR GeneID; 8623903; -.
DR KEGG; ddi:DDB_G0282921; -.
DR dictyBase; DDB_G0282921; ctu1.
DR eggNOG; KOG2840; Eukaryota.
DR HOGENOM; CLU_026481_1_0_1; -.
DR InParanoid; Q94480; -.
DR OMA; MNLDQKQ; -.
DR PhylomeDB; Q94480; -.
DR UniPathway; UPA00988; -.
DR PRO; PR:Q94480; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0002144; C:cytosolic tRNA wobble base thiouridylase complex; IBA:GO_Central.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IBA:GO_Central.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IMP:dictyBase.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_03053; CTU1; 1.
DR InterPro; IPR032442; CTU1_C.
DR InterPro; IPR000541; Ncs6/Tuc1/Ctu1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR011063; TilS/TtcA_N.
DR InterPro; IPR035107; tRNA_thiolation_TtcA_Ctu1.
DR InterPro; IPR020554; UPF0021_CS.
DR PANTHER; PTHR11807:SF12; PTHR11807:SF12; 1.
DR Pfam; PF01171; ATP_bind_3; 1.
DR Pfam; PF16503; zn-ribbon_14; 1.
DR PIRSF; PIRSF004976; ATPase_YdaO; 1.
DR TIGRFAMs; TIGR00269; TIGR00269; 1.
DR PROSITE; PS01263; UPF0021; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; RNA-binding; Transferase; tRNA processing;
KW tRNA-binding.
FT CHAIN 1..360
FT /note="Cytoplasmic tRNA 2-thiolation protein 1"
FT /id="PRO_0000219890"
FT REGION 327..360
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 119
FT /note="Q -> P (in Ref. 2; AAB06790)"
FT /evidence="ECO:0000305"
FT CONFLICT 122..123
FT /note="LK -> SQ (in Ref. 2; AAB06790)"
FT /evidence="ECO:0000305"
FT CONFLICT 150
FT /note="F -> S (in Ref. 2; AAB06790)"
FT /evidence="ECO:0000305"
FT CONFLICT 185..187
FT /note="LMN -> FMD (in Ref. 2; AAB06790)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="K -> R (in Ref. 2; AAB06790)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 360 AA; 40801 MW; F03699A316664910 CRC64;
MENTATKKKS KLCEVCNVSR PVLKRPKTGE MICKECFYTV FEDEIHHTII SNNLFKRGDR
VAIGASGGKD STVLAEIMTL LNKKYDYGLD LFLLSIDEGI TGYRDDSLET VKRNQEQYQI
PLKILSYKEL YNWTMDDIVK EIGLKGNCTF CGVFRRQALD RGAVMLKANK IVTGHNADDI
AETVLMNLLR GDIPRLQRCV NIITGSEGAL PRSKPFKYTY QKEIVMYAYF KKLDYFSTEC
IYAPNAYRGH ARDFLKDLEA VRPSIIIDII HSAENFHFRE ENKMPVQRNC IQCGYICSND
ICMACDLLKN LNSGLAKVKL SINLKRGKDN NNNSNNNDNN NTLKLESTSA ATTTTTTVSN
