ID   CTU1_RAT                Reviewed;         382 AA.
AC   B1WBV0;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 1 {ECO:0000255|HAMAP-Rule:MF_03053};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_03053};
DE   AltName: Full=ATP-binding domain-containing protein 3 {ECO:0000255|HAMAP-Rule:MF_03053};
DE   AltName: Full=Cytoplasmic tRNA adenylyltransferase 1 {ECO:0000255|HAMAP-Rule:MF_03053};
GN   Name=Ctu1; Synonyms=Atpbd3, Ncs6;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds
CC       tRNAs and probably acts by catalyzing adenylation of tRNAs, an
CC       intermediate required for 2-thiolation. It is unclear whether it acts
CC       as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1
CC       onto the uridine of tRNAs at wobble position. {ECO:0000255|HAMAP-
CC       Rule:MF_03053}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03053}.
CC   -!- SUBUNIT: Component of a complex at least composed of URM1, CTU2/NCS2
CC       and CTU1/ATPBD3. May form a heterodimer with CTU2/NCS2.
CC       {ECO:0000255|HAMAP-Rule:MF_03053}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03053}.
CC   -!- SIMILARITY: Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_03053}.
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DR   EMBL; CH473979; EDM07542.1; -; Genomic_DNA.
DR   EMBL; BC161898; AAI61898.1; -; mRNA.
DR   RefSeq; NP_001099721.1; NM_001106251.1.
DR   RefSeq; XP_006229046.1; XM_006228984.3.
DR   AlphaFoldDB; B1WBV0; -.
DR   SMR; B1WBV0; -.
DR   STRING; 10116.ENSRNOP00000024690; -.
DR   jPOST; B1WBV0; -.
DR   PaxDb; B1WBV0; -.
DR   PeptideAtlas; B1WBV0; -.
DR   Ensembl; ENSRNOT00000024690; ENSRNOP00000024690; ENSRNOG00000018334.
DR   GeneID; 292847; -.
DR   KEGG; rno:292847; -.
DR   UCSC; RGD:1308740; rat.
DR   CTD; 90353; -.
DR   RGD; 1308740; Ctu1.
DR   eggNOG; KOG2840; Eukaryota.
DR   GeneTree; ENSGT00390000001041; -.
DR   HOGENOM; CLU_026481_1_0_1; -.
DR   InParanoid; B1WBV0; -.
DR   OMA; MNLDQKQ; -.
DR   OrthoDB; 860739at2759; -.
DR   PhylomeDB; B1WBV0; -.
DR   TreeFam; TF352405; -.
DR   UniPathway; UPA00988; -.
DR   PRO; PR:B1WBV0; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Proteomes; UP000234681; Chromosome 1.
DR   Bgee; ENSRNOG00000018334; Expressed in pancreas and 20 other tissues.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0002144; C:cytosolic tRNA wobble base thiouridylase complex; IBA:GO_Central.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; ISS:UniProtKB.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03053; CTU1; 1.
DR   InterPro; IPR032442; CTU1_C.
DR   InterPro; IPR000541; Ncs6/Tuc1/Ctu1.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR011063; TilS/TtcA_N.
DR   InterPro; IPR035107; tRNA_thiolation_TtcA_Ctu1.
DR   PANTHER; PTHR11807:SF12; PTHR11807:SF12; 1.
DR   Pfam; PF01171; ATP_bind_3; 1.
DR   Pfam; PF16503; zn-ribbon_14; 1.
DR   PIRSF; PIRSF004976; ATPase_YdaO; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Phosphoprotein; Reference proteome; RNA-binding; Transferase;
KW   tRNA processing; tRNA-binding.
FT   CHAIN           1..382
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 1"
FT                   /id="PRO_0000368237"
FT   REGION          350..382
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         200
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q7Z7A3"
SQ   SEQUENCE   382 AA;  40242 MW;  E49417AF4CD281E0 CRC64;
     MPAPTCFSCH KARAALRRPR SGQALCGPCF CAAFEAEVLR TVLAGHLLPQ GAVVAVGASG
     GKDSTVLAHV LRELAPRLGI TLHLVAVDEG IGGYRDAALE AVRSQAARWE LPLTIVAYED
     LFGGWTMDAV ARSTAGSGRS RSCCTFCGVL RRRALEEGAR LVGATHIVTG HNADDMAETV
     LMNFLRGDAG RLARGGVLGS TGEGCALPRC RPLQFASQKE VVLYAHFRHL RYFSEECVYA
     PEAFRGHARD LLKLLEAARP SAVLDLVHSA ERLALAPAAK PPPPGTCSRC GALASNKLCQ
     ACALLDGLNR GLPRLAIGKG RRVLQVEPPP LGNPSRVTSD PVALPEPCTC KQSKDESNPC
     GNGGDRAGAT CVSKSDLSPV AE