ID   CTU1_XENLA              Reviewed;         341 AA.
AC   Q05AW7;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 1 {ECO:0000255|HAMAP-Rule:MF_03053};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_03053};
DE   AltName: Full=ATP-binding domain-containing protein 3 {ECO:0000255|HAMAP-Rule:MF_03053};
DE   AltName: Full=Cytoplasmic tRNA adenylyltransferase 1 {ECO:0000255|HAMAP-Rule:MF_03053};
GN   Name=ctu1; Synonyms=atpbd3, ncs6;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds
CC       tRNAs and probably acts by catalyzing adenylation of tRNAs, an
CC       intermediate required for 2-thiolation. It is unclear whether it acts
CC       as a sulfurtransferase that transfers sulfur from thiocarboxylated urm1
CC       onto the uridine of tRNAs at wobble position. {ECO:0000255|HAMAP-
CC       Rule:MF_03053}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03053}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03053}.
CC   -!- SIMILARITY: Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_03053}.
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DR   EMBL; BC123240; AAI23241.1; -; mRNA.
DR   RefSeq; NP_001090363.1; NM_001096894.1.
DR   AlphaFoldDB; Q05AW7; -.
DR   SMR; Q05AW7; -.
DR   BioGRID; 607981; 1.
DR   IntAct; Q05AW7; 1.
DR   MaxQB; Q05AW7; -.
DR   DNASU; 779274; -.
DR   GeneID; 779274; -.
DR   KEGG; xla:779274; -.
DR   CTD; 779274; -.
DR   Xenbase; XB-GENE-5872246; ctu1.L.
DR   OMA; MNLDQKQ; -.
DR   OrthoDB; 860739at2759; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000186698; Chromosome 1L.
DR   Bgee; 779274; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; ISS:UniProtKB.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03053; CTU1; 1.
DR   InterPro; IPR032442; CTU1_C.
DR   InterPro; IPR000541; Ncs6/Tuc1/Ctu1.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR011063; TilS/TtcA_N.
DR   InterPro; IPR035107; tRNA_thiolation_TtcA_Ctu1.
DR   InterPro; IPR020554; UPF0021_CS.
DR   PANTHER; PTHR11807:SF12; PTHR11807:SF12; 1.
DR   Pfam; PF01171; ATP_bind_3; 1.
DR   Pfam; PF16503; zn-ribbon_14; 1.
DR   PIRSF; PIRSF004976; ATPase_YdaO; 1.
DR   TIGRFAMs; TIGR00269; TIGR00269; 1.
DR   PROSITE; PS01263; UPF0021; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; RNA-binding; Transferase; tRNA processing;
KW   tRNA-binding.
FT   CHAIN           1..341
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 1"
FT                   /id="PRO_0000282394"
SQ   SEQUENCE   341 AA;  38497 MW;  D9F4B2F7A2282243 CRC64;
     MPVKCSSCEE WRAVLRRPKT GHSLCKDCFF YAFEEEIHNT IVSAKLFHPG EKVGIGASGG
     KDSTVLAHVM KVLNERYAYG LDLILLSVDE GISGYRDDSL QTVKRNQQQY ELPLKIVSYL
     ELYGWTMDQI VKQVGLKNNC TFCGVFRRQA LDRGAMMLGI NKICTGHNAD DIAETVLMNF
     LRGDIARLRR CTAITTGSEG AIPRCKPLKY TYEKEIVLYA YFKKLDYFST ECIYSPNAYR
     GHARVFLKDL EAIRPSSIID IIHSGENLSV NEDVRMPVQG TCTRCGYISS QSLCKACVLL
     EGLNRGLPKL GIGKHHKLHH KLLSQEPLSE QEERKLRAVV H