ID   CTU1_XENTR              Reviewed;         341 AA.
AC   Q5FW05;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 1 {ECO:0000255|HAMAP-Rule:MF_03053};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_03053};
DE   AltName: Full=ATP-binding domain-containing protein 3 {ECO:0000255|HAMAP-Rule:MF_03053};
DE   AltName: Full=Cytoplasmic tRNA adenylyltransferase 1 {ECO:0000255|HAMAP-Rule:MF_03053};
GN   Name=ctu1; Synonyms=atpbd3, ncs6;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds
CC       tRNAs and probably acts by catalyzing adenylation of tRNAs, an
CC       intermediate required for 2-thiolation. It is unclear whether it acts
CC       as a sulfurtransferase that transfers sulfur from thiocarboxylated urm1
CC       onto the uridine of tRNAs at wobble position. {ECO:0000255|HAMAP-
CC       Rule:MF_03053}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03053}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03053}.
CC   -!- SIMILARITY: Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_03053}.
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DR   EMBL; BC089678; AAH89678.1; -; mRNA.
DR   RefSeq; NP_001015743.1; NM_001015743.1.
DR   AlphaFoldDB; Q5FW05; -.
DR   SMR; Q5FW05; -.
DR   STRING; 8364.ENSXETP00000008736; -.
DR   PaxDb; Q5FW05; -.
DR   DNASU; 548460; -.
DR   Ensembl; ENSXETT00000008736; ENSXETP00000008736; ENSXETG00000004036.
DR   GeneID; 548460; -.
DR   KEGG; xtr:548460; -.
DR   CTD; 90353; -.
DR   Xenbase; XB-GENE-5872210; ctu1.
DR   eggNOG; KOG2840; Eukaryota.
DR   HOGENOM; CLU_026481_1_2_1; -.
DR   InParanoid; Q5FW05; -.
DR   OMA; CLHINLG; -.
DR   OrthoDB; 860739at2759; -.
DR   PhylomeDB; Q5FW05; -.
DR   TreeFam; TF352405; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000008143; Chromosome 1.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000004036; Expressed in egg cell and 15 other tissues.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0002144; C:cytosolic tRNA wobble base thiouridylase complex; IBA:GO_Central.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; ISS:UniProtKB.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03053; CTU1; 1.
DR   InterPro; IPR032442; CTU1_C.
DR   InterPro; IPR000541; Ncs6/Tuc1/Ctu1.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR011063; TilS/TtcA_N.
DR   InterPro; IPR035107; tRNA_thiolation_TtcA_Ctu1.
DR   InterPro; IPR020554; UPF0021_CS.
DR   PANTHER; PTHR11807:SF12; PTHR11807:SF12; 1.
DR   Pfam; PF01171; ATP_bind_3; 1.
DR   Pfam; PF16503; zn-ribbon_14; 1.
DR   PIRSF; PIRSF004976; ATPase_YdaO; 1.
DR   TIGRFAMs; TIGR00269; TIGR00269; 1.
DR   PROSITE; PS01263; UPF0021; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; RNA-binding; Transferase; tRNA processing;
KW   tRNA-binding.
FT   CHAIN           1..341
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 1"
FT                   /id="PRO_0000282395"
SQ   SEQUENCE   341 AA;  38384 MW;  1D85C7232AF35765 CRC64;
     MPVNCSSCEE RRAVLRRPKT GHSLCKDCFF HAFEEEIHHT IVSAKLFNPG EKVGIGASGG
     KDSTVLAHVL KVLNERYAYG LDLILVSVDE GISGYRDDSL ETVKRNQQQY ELPLKIVSYQ
     ELYGWTMDQI VKQVGLKNNC TFCGVFRRQA LDRGAMMLGV NKICTGHNAD DIAETVLMNF
     LRGDIARLRR CTSITTGSEG AIPRCKPLKY AYEKEIVLYA YFKKLDYFST ECIYSPNAYR
     GHARAFLKDL EAIRPSSIMD IIHSGENLSV KEDVRMPVQG TCTRCGYISS QSLCKACVLL
     EGLNRGLPKL GIGKHHKLHH KLLSQEPLSE QEERKLKAVD F