CTU2A_XENLA
ID CTU2A_XENLA Reviewed; 512 AA.
AC Q08B12; Q4V7Y1;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2-A {ECO:0000255|HAMAP-Rule:MF_03054};
GN Name=ctu2-a; Synonyms=ncs2-a;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo, and Ovary;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with ctu1/atpbd3 that ligates sulfur from
CC thiocarboxylated urm1 onto the uridine of tRNAs at wobble position.
CC {ECO:0000255|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC Rule:MF_03054}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH97664.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BC097664; AAH97664.1; ALT_INIT; mRNA.
DR EMBL; BC124921; AAI24922.1; -; mRNA.
DR RefSeq; NP_001167511.1; NM_001174040.1.
DR AlphaFoldDB; Q08B12; -.
DR BioGRID; 1079194; 1.
DR IntAct; Q08B12; 1.
DR DNASU; 100381163; -.
DR GeneID; 100381163; -.
DR KEGG; xla:100381163; -.
DR CTD; 348180; -.
DR Xenbase; XB-GENE-6466467; ctu2.L.
DR OrthoDB; 1442062at2759; -.
DR UniPathway; UPA00988; -.
DR Proteomes; UP000186698; Chromosome 4L.
DR Bgee; 100381163; Expressed in egg cell and 19 other tissues.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; PTHR20882; 1.
DR Pfam; PF10288; CTU2; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; tRNA processing.
FT CHAIN 1..512
FT /note="Cytoplasmic tRNA 2-thiolation protein 2-A"
FT /id="PRO_0000289180"
FT CONFLICT 456
FT /note="P -> H (in Ref. 1; AAH97664)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 512 AA; 57231 MW; 42B1184BD32CE7D3 CRC64;
MCEEGETYCP EVKDAKQGKS LGKICMKCKE SSAALLIRAG DAFCKSCFKE YFVHKFRATL
GKNRVIYPGE KVLLAYSGGP SSSAMVRQVQ EGLSRDAPKK LRFVPGILFI DEGTACGMSW
EERQQILSEI CSVLQQTKIP FHIVSLEQVF SLPGSVLQRG APEQRPNYKE EVDRFLVQER
EQGDAGCSEM LERLEVTDSD SPGSSDKMYQ STCSHPPDMH TQKLKQLFAS AKTLTAKQQL
LHTLRSHLIL HIARTCGYSK VMTGESCTRL SIRLLSNVSL GRGAFLPLDT GFCDSRYGDV
DIIRPMREYS SKEIAYYNRF FNVSPIFIPA LDTKASENSS IQHLTEVFVN RLQADFPSTV
STLYRTSEKL NVSKIDADQE TCAKDRCLLC LSPLDTQAGK ASAFSATQLS HHLSQKIPMK
SNDLANNSDK SCCQGGQGCK EAGYGDTCQS RALQTPSFVH MLCYSCRLTV KDMQSLDVLP
QYVLHEAEHR CHRTEMRKEI QEFLLDEDDG DS