ID   CTU2_ARATH              Reviewed;         458 AA.
AC   O65628; Q058N5; Q680D0; Q8LES1;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 3.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   Name=CTU2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   Synonyms=NCS2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   OrderedLocusNames=At4g35910; ORFNames=T19K4.40;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA18485.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAA21477.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAB81500.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AL022373; CAA18485.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL031986; CAA21477.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL161588; CAB81500.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002687; AEE86588.1; -; Genomic_DNA.
DR   EMBL; AK175937; BAD43700.1; -; mRNA.
DR   EMBL; AK220738; BAD93893.1; -; mRNA.
DR   EMBL; BT029183; ABJ17118.1; -; mRNA.
DR   EMBL; AY085266; AAM62498.1; -; mRNA.
DR   PIR; T04701; T04701.
DR   RefSeq; NP_567993.1; NM_119758.3.
DR   AlphaFoldDB; O65628; -.
DR   SMR; O65628; -.
DR   BioGRID; 15028; 2.
DR   STRING; 3702.AT4G35910.1; -.
DR   iPTMnet; O65628; -.
DR   PaxDb; O65628; -.
DR   PRIDE; O65628; -.
DR   ProteomicsDB; 220457; -.
DR   EnsemblPlants; AT4G35910.1; AT4G35910.1; AT4G35910.
DR   GeneID; 829746; -.
DR   Gramene; AT4G35910.1; AT4G35910.1; AT4G35910.
DR   KEGG; ath:AT4G35910; -.
DR   Araport; AT4G35910; -.
DR   TAIR; locus:2125359; AT4G35910.
DR   eggNOG; KOG2594; Eukaryota.
DR   HOGENOM; CLU_048050_0_0_1; -.
DR   InParanoid; O65628; -.
DR   OMA; YVRHKFR; -.
DR   OrthoDB; 1442062at2759; -.
DR   UniPathway; UPA00988; -.
DR   PRO; PR:O65628; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; O65628; baseline and differential.
DR   Genevisible; O65628; AT.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016783; F:sulfurtransferase activity; IMP:TAIR.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0010311; P:lateral root formation; IMP:TAIR.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; IMP:TAIR.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IBA:GO_Central.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..458
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369282"
FT   CONFLICT        172
FT                   /note="P -> Q (in Ref. 5; AAM62498)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        187
FT                   /note="R -> G (in Ref. 3; BAD43700/BAD93893)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   458 AA;  50399 MW;  DD687DAD5C3AE839 CRC64;
     MACNSSGCES GCYDREKDNG SKIVDDAVSG GGNHESVCVK CKCNAPMTFG DGGFDDGRFC
     ADCFRNNVFG KFRLAVTSHA MITPSDNVLV AFSGGSSSRV SLQFVHELQI KALKNYEASR
     DRSLPVFGVG VAFVDETAAF PALSTEMIDA IEWVRYTVSC LSPPAKDLHV VPVESIFGSD
     SLDARDRLLK LLDSVPDDTG KEDLLLHLKM LSLQKVAAEN GYNRLVLGSC TSRIASHVLT
     ATVKGRGYSL SADIQHVDAR WKVPIVLPLR DCVRLEITRL CLLDGLKTVE LACRSQCGIN
     DLVSSFVALL QEENPSRECT IVRTAAKLTP FYFNKIPETD DSNVPMATQR RLKRFNLKYD
     GSMTTEAFCP ICNGPLNRSD SSELDTFEEG QESDVLYAAC CSSCRFQILP QDGSSLEQFS
     SFLPDHMISQ VKHQKVDSQA YLREKIKDCL LLDDEEVV