ID   CTU2_DROAN              Reviewed;         403 AA.
AC   B3MN57;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   ORFNames=GF15629;
OS   Drosophila ananassae (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7217;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14024-0371.13;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
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DR   EMBL; CH902620; EDV32035.1; -; Genomic_DNA.
DR   RefSeq; XP_001962814.1; XM_001962778.2.
DR   AlphaFoldDB; B3MN57; -.
DR   SMR; B3MN57; -.
DR   STRING; 7217.FBpp0118821; -.
DR   EnsemblMetazoa; FBtr0120329; FBpp0118821; FBgn0092653.
DR   GeneID; 6498435; -.
DR   KEGG; dan:6498435; -.
DR   eggNOG; KOG2594; Eukaryota.
DR   HOGENOM; CLU_024534_2_1_1; -.
DR   InParanoid; B3MN57; -.
DR   OMA; YVRHKFR; -.
DR   OrthoDB; 1442062at2759; -.
DR   PhylomeDB; B3MN57; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000007801; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..403
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369270"
SQ   SEQUENCE   403 AA;  44278 MW;  DB5D8729EED21CF2 CRC64;
     MCSIGEDDFG DEGGTHAMVA GSVPSGIDLS PGECSKCDIS SDELFQLNFR TPECRDCFLA
     YVRHKFRAAL GAAKVLPRDA EVLLVVDGSA ESLVLLDMLY YAQTQNTFKR LHCSARMVYL
     EDQPVQGRDP ADLESLEALS KRYEAFEFYV ITLGLPVGSL KLLKDYSPVA QESNELIQKL
     SQLRSLTSRQ DYLQQQRKNL IASVAQKLHC SHVFNSSISV DLATQLLTSI ALGRGGSAAL
     DVALLDDRLA AGIKLLRPLK DLTEQEVQFY VHAQRLQPLL MASSRYGQEL GDAASLQNLT
     SAFVANLQKN FSSTVSTVFR TGGKISESTH PEQAKCNHCQ SALDVELSDT LLAIEYSRSV
     SEAGVRLNEA IEDPEIISKR RMKLNDELCH ACSSIEADFK NGS