ID   CTU2_DROER              Reviewed;         404 AA.
AC   B3NM45;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   ORFNames=GG21627;
OS   Drosophila erecta (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7220;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14021-0224.01;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
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DR   EMBL; CH954179; EDV54645.1; -; Genomic_DNA.
DR   RefSeq; XP_001974245.1; XM_001974209.2.
DR   AlphaFoldDB; B3NM45; -.
DR   SMR; B3NM45; -.
DR   STRING; 7220.FBpp0140173; -.
DR   EnsemblMetazoa; FBtr0141681; FBpp0140173; FBgn0113805.
DR   GeneID; 6549283; -.
DR   KEGG; der:6549283; -.
DR   eggNOG; KOG2594; Eukaryota.
DR   HOGENOM; CLU_024534_2_1_1; -.
DR   OMA; YVRHKFR; -.
DR   OrthoDB; 1442062at2759; -.
DR   PhylomeDB; B3NM45; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000008711; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..404
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369271"
SQ   SEQUENCE   404 AA;  44522 MW;  C829614FA86FF48F CRC64;
     MCSIGEDDFG DEGAAHAMVV EALPLGIVLS PGNCSKCDVN SGELYKLNFR AAECRECFLT
     YARHKFRAAL GAAKILPRNA EVLLVLDGSA KSLVLLDMLH FAQTQNQFKR LHCNARVVYV
     EEQQVQDRDP VDLEALLSLS VQYAPFDFYV IELGQGCSLQ RIKDYSPSLK ANNELIHKLQ
     KLQSLTARQD YLQQQRKNLI CSVAQSLQCT HVFEPNISVD LATQLLTAIA LGRGASAALD
     VALLDDRLSG DVKLLRPLKD LSEQEIQFYI HAQRLKPLFQ SGSHYGMERG PTASLQNLTS
     AFVANLQHNY ASTVSTVFRT GDKIAVNSDP EQATCVHCQS ALDSELSDTL LAIEYSRSVS
     EAGVSLYKNG QDLESLAKKR LEMQDGLCHA CRAIQAELES GNLL