ID   CTU2_DROGR              Reviewed;         397 AA.
AC   B4JCV8;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   ORFNames=GH10624;
OS   Drosophila grimshawi (Hawaiian fruit fly) (Idiomyia grimshawi).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Hawaiian Drosophila.
OX   NCBI_TaxID=7222;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 15287-2541.00;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
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DR   EMBL; CH916368; EDW03197.1; -; Genomic_DNA.
DR   RefSeq; XP_001988330.1; XM_001988294.1.
DR   AlphaFoldDB; B4JCV8; -.
DR   SMR; B4JCV8; -.
DR   STRING; 7222.FBpp0144530; -.
DR   EnsemblMetazoa; FBtr0146038; FBpp0144530; FBgn0118105.
DR   GeneID; 6562494; -.
DR   KEGG; dgr:6562494; -.
DR   eggNOG; KOG2594; Eukaryota.
DR   HOGENOM; CLU_024534_2_1_1; -.
DR   InParanoid; B4JCV8; -.
DR   OMA; YVRHKFR; -.
DR   OrthoDB; 1442062at2759; -.
DR   PhylomeDB; B4JCV8; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000001070; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..397
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369272"
SQ   SEQUENCE   397 AA;  43383 MW;  618672E7698F8C8F CRC64;
     MCSIGEDDFG DEGATHAMLP GSSATGTVLS AGSCSKCNLA SDELYKLNFR AAECQLCFLS
     YARHKFRAAL GAAKALPRSA EVLLLVDGSG ASLVLLDMLH FAQTQNTFKR LHCNARVLYI
     DACGNDSLSS LHKLHERYAP FEFYVIQLNA EPKSLQSLKE YSASIVNATQ QELRSLTARQ
     DYGLQQRKRL IGAVAAHLQC SHVFESSISS TLATQLLTSV ALGRGGSVAL DVALLDDRLQ
     DDIKLLRPLK DLNEQEVQFY VHAQQLQPFT SENELDQSSA ASLQNLTSAF VANLQINYAS
     TVSTIFRTGD KIAAKQQTNV DPTTTCTLCH SVLDHQLSDT LLAIEYSRAV SEMGVALQQH
     DNMEALEQRA RQRLTAAQDL CHACRNIQAE LTSGNIP