ID   CTU2_DROMO              Reviewed;         404 AA.
AC   B4KIW3;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   ORFNames=GI19780;
OS   Drosophila mojavensis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila.
OX   NCBI_TaxID=7230;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 15081-1352.22;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
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DR   EMBL; CH933807; EDW13476.1; -; Genomic_DNA.
DR   RefSeq; XP_002004034.1; XM_002003998.2.
DR   AlphaFoldDB; B4KIW3; -.
DR   SMR; B4KIW3; -.
DR   STRING; 7230.FBpp0168997; -.
DR   EnsemblMetazoa; FBtr0170505; FBpp0168997; FBgn0142517.
DR   GeneID; 6578112; -.
DR   KEGG; dmo:Dmoj_GI19780; -.
DR   eggNOG; KOG2594; Eukaryota.
DR   HOGENOM; CLU_024534_2_1_1; -.
DR   InParanoid; B4KIW3; -.
DR   OMA; YVRHKFR; -.
DR   OrthoDB; 1442062at2759; -.
DR   PhylomeDB; B4KIW3; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000009192; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..404
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369273"
SQ   SEQUENCE   404 AA;  45052 MW;  0C6F5E9F33C8B7D2 CRC64;
     MCSIGEDDFG DEGGTHAMLP SCSETGIVLR AGDCSKCGQS SQQLYKLNFR SAECSECFLK
     YVRHKFRAAL GAAKVLPRNA EILLVIDGSS ESLVLLDMVH FAQTQNTFKR LHCNARVLYV
     DAHCSPIKEE ESPLLALNEL QRRYEPFEFY VIQLNGDPQS LQLLKDYTVR QLDSILDQFN
     SSTSRQDYMQ QKRKFLVGGV AMKLKCSHVF EPSISPSLAT QLLTSVALGR GASVALDVAL
     LDDRLMGDVK LLRPLKDLND QEVQFYVHAK ELKAFRVDDP SHYPLSGGSL QSLTRDFVAN
     LQLNYASTVS TVFRTGDKIA PKRNSKEIMA SYCALCQSLL DNNISDTLLA IEYSRAVSEV
     GVELKMKSSC DVLEQRVLER LNEKEELCHA CRNIQAELSS GTLI