ID   CTU2_DROPE              Reviewed;         405 AA.
AC   B4GWY0;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   ORFNames=GL21098;
OS   Drosophila persimilis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7234;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSH-3 / Tucson 14011-0111.49;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
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DR   EMBL; CH479195; EDW27307.1; -; Genomic_DNA.
DR   RefSeq; XP_002023179.1; XM_002023143.1.
DR   AlphaFoldDB; B4GWY0; -.
DR   SMR; B4GWY0; -.
DR   STRING; 7234.FBpp0185205; -.
DR   EnsemblMetazoa; FBtr0186713; FBpp0185205; FBgn0158693.
DR   GeneID; 6597944; -.
DR   KEGG; dpe:6597944; -.
DR   eggNOG; KOG2594; Eukaryota.
DR   HOGENOM; CLU_024534_2_1_1; -.
DR   OMA; YVRHKFR; -.
DR   PhylomeDB; B4GWY0; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000008744; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..405
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369274"
SQ   SEQUENCE   405 AA;  43911 MW;  4C1C6C18A7E1B331 CRC64;
     MCSIGEDDVG DEGATHAMVA ESLPTGIVIS PGDCNKCGVV SSELYKLNFR VAECRDCFLN
     HARHKFRASL GAAKVLPRNA EVLLAVDGSA ESLVLLDMLH FAQTQNTFRR LHCNARVVYI
     DDQSVHGGES MNLQALQALG TRYEPLEFYV VELGASACSL QRLGQYSTSL KEPNGLNTKL
     EKLRSLTARQ DYHQQQRKNL LASVAQKLSC SHVFEPSVSG DLATQLLTSI ALGRGGSAAL
     DVALLDDRLA AGVKLLRPLR DLNEQEVRFY VHACQLKPLR ESGSSYGQER GQTASLQNLT
     AAFVGNLQQN YPATVSTVFR TGDKIAANAH MEQASCAQCQ SPLDAKLSDT LLAIEYSRAV
     SEAGVGLSKD GDASESLAKQ RLEFKDGLCH ACRCIQLELG YDTLS