ID   CTU2_DROPS              Reviewed;         405 AA.
AC   Q29K59;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 2.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   ORFNames=GA10142;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25 / Tucson 14011-0121.94;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA   Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA   Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA   Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA   Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA   Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA   Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT   gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
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DR   EMBL; CH379061; EAL33319.2; -; Genomic_DNA.
DR   RefSeq; XP_001356256.2; XM_001356220.3.
DR   AlphaFoldDB; Q29K59; -.
DR   SMR; Q29K59; -.
DR   STRING; 7237.FBpp0287995; -.
DR   EnsemblMetazoa; FBtr0289557; FBpp0287995; FBgn0070201.
DR   GeneID; 4816297; -.
DR   KEGG; dpo:Dpse_GA10142; -.
DR   eggNOG; KOG2594; Eukaryota.
DR   HOGENOM; CLU_024534_2_1_1; -.
DR   InParanoid; Q29K59; -.
DR   OMA; YVRHKFR; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000001819; Chromosome 4.
DR   Bgee; FBgn0070201; Expressed in male reproductive system and 2 other tissues.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..405
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369275"
SQ   SEQUENCE   405 AA;  43960 MW;  58DC0CB7F109BF23 CRC64;
     MCSIGEDDFG DEGATHAMVA ESLPTGIVIS PGDCNKCGVV SSELYKLNFR VAECRDCFLN
     HARHKFRASL GAAKVLPRNA EVLLAVDGSA ESLVLLDMLH FAQTQNTFRR LHCNARVVYI
     DDQSVHGGES MNLQALQALG TRYEPLEFYV VELGASACSL QRLGQYSTSL KEPNGLNTKL
     EKLRSLTARQ DYHQQQRKNL LASVAQKLSC SHVFEPSVSG DLAAQLLTSI TLGRGGSAAL
     DVALLDDRLA AGVKLLRPLR DLNEQEVRFY VHACQLKPLR ESGSSYGQER GQTASLQNLT
     AAFVGNLQQN YPATVSTVFR TGDKIAANAH MEQASCAQCQ SPLDAKLSDT LLANEYSRAV
     SEAGVGLSKD GDASESLAKQ RLEFKDGLCH ACRCIQLELG YDTLS