ID   CTU2_DROSE              Reviewed;         405 AA.
AC   B4I5W3;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   ORFNames=GM17005;
OS   Drosophila sechellia (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Rob3c / Tucson 14021-0248.25;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
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DR   EMBL; CH480822; EDW55769.1; -; Genomic_DNA.
DR   RefSeq; XP_002039123.1; XM_002039087.1.
DR   AlphaFoldDB; B4I5W3; -.
DR   SMR; B4I5W3; -.
DR   STRING; 7238.B4I5W3; -.
DR   EnsemblMetazoa; FBtr0199990; FBpp0198482; FBgn0171918.
DR   GeneID; 6614696; -.
DR   KEGG; dse:6614696; -.
DR   HOGENOM; CLU_024534_2_1_1; -.
DR   OMA; YVRHKFR; -.
DR   PhylomeDB; B4I5W3; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000001292; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..405
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369276"
SQ   SEQUENCE   405 AA;  44644 MW;  49D72608436F499B CRC64;
     MCSIGEDDFG DDGATHAMVV ESLPLGIVLS PGNCSKCDVN SGELYKLNFR TAECRECFLA
     YARHKFRAAL GAAKILPRNA EVLLVLDGSA ESLVLLDMLH FAQTQNTFKR LHCNARVVYV
     EEQKVQGRDP VHLEALQNLS TQYAPFDFYV IELGALPSSM QRIKDYSPSL NANNELIHKL
     QKLRSLTARQ DYLQQQRKNL ICSVAQCLQC THVFESNISV DLATQLLTAI ALGRGGSAAL
     DVALLDDRLS GDVKLLRPLK DLNEQEIQFY IHAQRLKPLF EKGSCFGREH GQTASLQNLT
     SAFVANLQQN YASTVSTVFR TGDKIAANSN PEQSSCVHCR STLDSEISDT LLAIEYSRTV
     SEAGVSLYKS GQDLEGLAKK RLENKDGLCH ACRAIQAELD SGNLL