ID   CTU2_DROSI              Reviewed;         405 AA.
AC   B4Q9Z1;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000255|HAMAP-Rule:MF_03054};
GN   ORFNames=GD21754;
OS   Drosophila simulans (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7240;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03054}.
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DR   EMBL; CM000361; EDX05521.1; -; Genomic_DNA.
DR   RefSeq; XP_002079936.1; XM_002079900.2.
DR   AlphaFoldDB; B4Q9Z1; -.
DR   SMR; B4Q9Z1; -.
DR   STRING; 7240.B4Q9Z1; -.
DR   EnsemblMetazoa; FBtr0221664; FBpp0220156; FBgn0193176.
DR   GeneID; 6732823; -.
DR   HOGENOM; CLU_024534_2_1_1; -.
DR   OMA; YVRHKFR; -.
DR   PhylomeDB; B4Q9Z1; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000000304; Chromosome 2l.
DR   Bgee; FBgn0193176; Expressed in embryo and 3 other tissues.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0034227; P:tRNA thio-modification; ISS:UniProtKB.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; ISS:UniProtKB.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; PTHR20882; 1.
DR   Pfam; PF10288; CTU2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..405
FT                   /note="Cytoplasmic tRNA 2-thiolation protein 2"
FT                   /id="PRO_0000369277"
SQ   SEQUENCE   405 AA;  44798 MW;  FB121275B78D9627 CRC64;
     MCSIGEDDFG DDGAAHAMAV ESLPLGIVLS PGNCSKCDVN SDELYKLNFR TAECRECFLA
     YARHKFRAAL GAAKILPRNA EVLLVLDGSA ESLVLLDMLH FAQTQNTFKR LHCNARVVYV
     EEQQVQGRDP VHLEALQNLS TQYAPFDFYV IELGALPSSM QRIKDYSPSL NANNELIHKL
     QKLRSLTARQ DYLQQQRKNL ICSVAQCLRC THVFESNISV DLATQLLTAI ALGRGGSAAL
     DVALLDDRLS GDVKLLRPLK DLNEQEIQFY IHAQRLKPLF QKGSRYGREH GQTASLQNLT
     SAFVANLQQN YASTVSTVFR TGDKIAANSN PEQSSCVHCR STLDTELSDT LLAIEYSRSV
     SEAGVSLYKS GQDLEDLAKK RLENKDGLCH ACRAIQAELD SGNLL